Restriction map for
pF1KB7705
(Linear) (Six-base) MAPSORT of: KIBB7705.seq Check: 7177 from: 1 to: 1257
KIBB7705.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AccI GT'mk_AC
Cuts at: 0 212 1257
Size: 212 1045
Acc65I G'GTAC_C
Cuts at: 0 489 1257
Size: 489 768
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 1257
Size: 109 1148
AflII C'TTAA_G
Cuts at: 0 244 516 594 1257
Size: 244 272 78 663
Fragments arranged by size:
663 272 244 78
AleI CACnn'nnGTG
Cuts at: 0 314 1257
Size: 314 943
AlwNI CAG_nnn'CTG
Cuts at: 0 1086 1257
Size: 1086 171
BanI G'GyrC_C
Cuts at: 0 489 1257
Size: 489 768
BanII G_rGCy'C
Cuts at: 0 922 1257
Size: 922 335
BbsI GAAGACnn'nnnn_
Cuts at: 0 503 1257
Size: 503 754
BbvCI CC'TCA_GC
Cuts at: 0 1079 1257
Size: 1079 178
BclI T'GATC_A
Cuts at: 0 147 162 1257
Size: 147 15 1095
Fragments arranged by size:
1095 147 15
Bme1580I G_kGCm'C
Cuts at: 0 1008 1257
Size: 1008 249
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 941 1257
Size: 941 316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 1257
Size: 419 838
Bpu10I CC'TnA_GC
Cuts at: 0 1079 1257
Size: 1079 178
BsaAI yAC'GTr
Cuts at: 0 75 1257
Size: 75 1182
BsaBI GATnn'nnATC
Cuts at: 0 146 1257
Size: 146 1111
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1002 1032 1257
Size: 1002 30 225
Fragments arranged by size:
1002 225 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 912 1257
Size: 912 345
BseYI C'CCAG_C
Cuts at: 0 1021 1050 1056 1257
Size: 1021 29 6 201
Fragments arranged by size:
1021 201 29 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1072 1257
Size: 1072 185
BsiHKAI G_wGCw'C
Cuts at: 0 585 922 1018 1257
Size: 585 337 96 239
Fragments arranged by size:
585 337 239 96
Bsp1286I G_dGCh'C
Cuts at: 0 585 922 1008 1018 1257
Size: 585 337 86 10 239
Fragments arranged by size:
585 337 239 86 10
BspHI T'CATG_A
Cuts at: 0 857 1257
Size: 857 400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 973 1257
Size: 973 284
BsrFI r'CCGG_y
Cuts at: 0 1065 1257
Size: 1065 192
BssSI C'ACGA_G
Cuts at: 0 1011 1257
Size: 1011 246
BtgI C'CryG_G
Cuts at: 0 608 1257
Size: 608 649
BtsI GCAGTG_nn'
Cuts at: 0 447 999 1092 1257
Size: 447 552 93 165
Fragments arranged by size:
552 447 165 93
ClaI AT'CG_AT
Cuts at: 0 330 1257
Size: 330 927
EaeI y'GGCC_r
Cuts at: 0 946 1257
Size: 946 311
EarI CTCTTCn'nnn_
Cuts at: 0 128 573 1257
Size: 128 445 684
Fragments arranged by size:
684 445 128
EcoICRI GAG'CTC
Cuts at: 0 920 1257
Size: 920 337
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 419 1257
Size: 109 310 838
Fragments arranged by size:
838 310 109
EcoO109I rG'GnC_Cy
Cuts at: 0 227 723 1074 1257
Size: 227 496 351 183
Fragments arranged by size:
496 351 227 183
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 620 1257
Size: 620 637
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 154 181 186 213 572 604 1257
Size: 154 27 5 27 359 32 653
Fragments arranged by size:
653 359 154 32 27 27 5
HindIII A'AGCT_T
Cuts at: 0 241 1257
Size: 241 1016
KpnI G_GTAC'C
Cuts at: 0 493 1257
Size: 493 764
MslI CAynn'nnrTG
Cuts at: 0 314 414 1160 1257
Size: 314 100 746 97
Fragments arranged by size:
746 314 100 97
MspA1I CmG'CkG
Cuts at: 0 437 1257
Size: 437 820
NcoI C'CATG_G
Cuts at: 0 608 1257
Size: 608 649
NdeI CA'TA_TG
Cuts at: 0 383 1145 1257
Size: 383 762 112
Fragments arranged by size:
762 383 112
PflMI CCAn_nnn'nTGG
Cuts at: 0 709 1257
Size: 709 548
PfoI T'CCnGG_A
Cuts at: 0 1070 1257
Size: 1070 187
PmlI CAC'GTG
Cuts at: 0 75 1257
Size: 75 1182
PpuMI rG'GwC_Cy
Cuts at: 0 227 723 1074 1257
Size: 227 496 351 183
Fragments arranged by size:
496 351 227 183
PstI C_TGCA'G
Cuts at: 0 442 1257
Size: 442 815
SacI G_AGCT'C
Cuts at: 0 922 1257
Size: 922 335
SanDI GG'GwC_CC
Cuts at: 0 723 1257
Size: 723 534
SapI GCTCTTCn'nnn_
Cuts at: 0 128 573 1257
Size: 128 445 684
Fragments arranged by size:
684 445 128
SfcI C'TryA_G
Cuts at: 0 438 570 969 1257
Size: 438 132 399 288
Fragments arranged by size:
438 399 288 132
SmlI C'TyrA_G
Cuts at: 0 244 516 594 1257
Size: 244 272 78 663
Fragments arranged by size:
663 272 244 78
StyI C'CwwG_G
Cuts at: 0 185 608 718 1257
Size: 185 423 110 539
Fragments arranged by size:
539 423 185 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 182 1257
Size: 182 1075
TatI w'GTAC_w
Cuts at: 0 1112 1257
Size: 1112 145
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1002 1034 1257
Size: 1002 32 223
Fragments arranged by size:
1002 223 32
Enzymes that do cut:
AccI Acc65I AcuI AflII AleI AlwNI BanI BanII
BbsI BbvCI BclI Bme1580I BplI BpmI Bpu10I BsaAI
BsaBI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BspMI BsrFI BssSI BtgI BtsI ClaI EaeI EarI
EcoICRI Eco57MI EcoO109I FalI Hin4I HindIII KpnI MslI
MspA1I NcoI NdeI PflMI PfoI PmlI PpuMI PstI
SacI SanDI SapI SfcI SmlI StyI TaqII TatI
TstI
Enzymes that do not cut:
AarI AatII AclI AfeI AflIII AgeI AhdI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BcgI BciVI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BpuEI BsaI BsaHI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrDI
BsrGI BssHII BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI CspCI DraI DraIII DrdI EagI EciI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HpaI KasI MfeI MluI MmeI MscI NaeI NarI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI