Restriction map for
pF1KB7702
(Linear) (Six-base) MAPSORT of: KIBB7702.seq Check: 5870 from: 1 to: 1383
KIBB7702.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 477 833 1383
Size: 477 356 550
Fragments arranged by size:
550 477 356
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1113 1383
Size: 1113 270
AfeI AGC'GCT
Cuts at: 0 190 1383
Size: 190 1193
AflIII A'CryG_T
Cuts at: 0 85 140 1383
Size: 85 55 1243
Fragments arranged by size:
1243 85 55
AhdI GACnn_n'nnGTC
Cuts at: 0 57 1383
Size: 57 1326
AleI CACnn'nnGTG
Cuts at: 0 843 1383
Size: 843 540
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 861 985 1091 1351 1366 1383
Size: 861 124 106 260 15 17
Fragments arranged by size:
861 260 124 106 17 15
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1129 1161 1383
Size: 1129 32 222
Fragments arranged by size:
1129 222 32
AlwNI CAG_nnn'CTG
Cuts at: 0 690 1059 1254 1257 1383
Size: 690 369 195 3 126
Fragments arranged by size:
690 369 195 126 3
ApaI G_GGCC'C
Cuts at: 0 312 371 498 662 726 1284 1383
Size: 312 59 127 164 64 558 99
Fragments arranged by size:
558 312 164 127 99 64 59
ApaLI G'TGCA_C
Cuts at: 0 144 1383
Size: 144 1239
ApoI r'AATT_y
Cuts at: 0 1243 1383
Size: 1243 140
BanI G'GyrC_C
Cuts at: 0 381 523 1383
Size: 381 142 860
Fragments arranged by size:
860 381 142
BanII G_rGCy'C
Cuts at: 0 312 371 446 498 647 662 677 726
Size: 312 59 75 52 149 15 15 49
Cuts at: 726 752 1024 1284 1383
Size: 26 272 260 99
Fragments arranged by size:
312 272 260 149 99 75 59 52
49 26 15 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 846 1383
Size: 846 537
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 363 397 1383
Size: 363 34 986
Fragments arranged by size:
986 363 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1015 1383
Size: 1015 368
Bme1580I G_kGCm'C
Cuts at: 0 40 148 312 371 386 498 521 528
Size: 40 108 164 59 15 112 23 7
Cuts at: 528 662 726 840 1284 1383
Size: 134 64 114 444 99
Fragments arranged by size:
444 164 134 114 112 108 99 64
59 40 23 15 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 490 843 1383
Size: 490 353 540
Fragments arranged by size:
540 490 353
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 666 1383
Size: 666 717
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 674 718 886 924 1383
Size: 379 295 44 168 38 459
Fragments arranged by size:
459 379 295 168 44 38
Bpu10I CC'TnA_GC
Cuts at: 0 440 671 1178 1285 1383
Size: 440 231 507 107 98
Fragments arranged by size:
507 440 231 107 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1096 1383
Size: 1096 287
BsaAI yAC'GTr
Cuts at: 0 86 143 414 1383
Size: 86 57 271 969
Fragments arranged by size:
969 271 86 57
BsaWI w'CCGG_w
Cuts at: 0 405 1383
Size: 405 978
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1294 1324 1383
Size: 1294 30 59
Fragments arranged by size:
1294 59 30
BseYI C'CCAG_C
Cuts at: 0 424 472 498 1383
Size: 424 48 26 885
Fragments arranged by size:
885 424 48 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 816 1015 1383
Size: 142 674 199 368
Fragments arranged by size:
674 368 199 142
BsiHKAI G_wGCw'C
Cuts at: 0 148 677 1383
Size: 148 529 706
Fragments arranged by size:
706 529 148
BsiWI C'GTAC_G
Cuts at: 0 11 629 1383
Size: 11 618 754
Fragments arranged by size:
754 618 11
Bsp1286I G_dGCh'C
Cuts at: 0 40 148 312 371 386 446 498 521
Size: 40 108 164 59 15 60 52 23
Cuts at: 521 528 647 662 677 726 752 840 1024
Size: 7 119 15 15 49 26 88 184
Cuts at: 1024 1284 1383
Size: 260 99
Fragments arranged by size:
260 184 164 119 108 99 88 60
59 52 49 40 26 23 15 15
15 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 477 833 1358 1383
Size: 477 356 525 25
Fragments arranged by size:
525 477 356 25
BsrFI r'CCGG_y
Cuts at: 0 460 774 1091 1383
Size: 460 314 317 292
Fragments arranged by size:
460 317 314 292
BsrGI T'GTAC_A
Cuts at: 0 293 1383
Size: 293 1090
BssHII G'CGCG_C
Cuts at: 0 249 1383
Size: 249 1134
BstAPI GCAn_nnn'nTGC
Cuts at: 0 690 1257 1383
Size: 690 567 126
Fragments arranged by size:
690 567 126
BstEII G'GTnAC_C
Cuts at: 0 975 1383
Size: 975 408
BstXI CCAn_nnnn'nTGG
Cuts at: 0 453 1383
Size: 453 930
BtgI C'CryG_G
Cuts at: 0 509 528 1383
Size: 509 19 855
Fragments arranged by size:
855 509 19
BtsI GCAGTG_nn'
Cuts at: 0 502 1230 1383
Size: 502 728 153
Fragments arranged by size:
728 502 153
DraIII CAC_nnn'GTG
Cuts at: 0 1217 1383
Size: 1217 166
EaeI y'GGCC_r
Cuts at: 0 270 455 512 567 803 872 1007 1383
Size: 270 185 57 55 236 69 135 376
Fragments arranged by size:
376 270 236 185 135 69 57 55
EarI CTCTTCn'nnn_
Cuts at: 0 1132 1383
Size: 1132 251
EcoICRI GAG'CTC
Cuts at: 0 675 1383
Size: 675 708
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 674 718 886 924 1113 1383
Size: 379 295 44 168 38 189 270
Fragments arranged by size:
379 295 270 189 168 44 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1270 1383
Size: 1270 113
EcoO109I rG'GnC_Cy
Cuts at: 0 21 309 367 368 450 494 495 658
Size: 21 288 58 1 82 44 1 163
Cuts at: 658 1281 1383
Size: 623 102
Fragments arranged by size:
623 288 163 102 82 58 44 21
1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 123 1383
Size: 123 1260
HaeII r_GCGC'y
Cuts at: 0 192 1383
Size: 192 1191
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 75 1383
Size: 43 32 1308
Fragments arranged by size:
1308 43 32
MscI TGG'CCA
Cuts at: 0 1009 1383
Size: 1009 374
MslI CAynn'nnrTG
Cuts at: 0 514 843 1165 1360 1383
Size: 514 329 322 195 23
Fragments arranged by size:
514 329 322 195 23
MspA1I CmG'CkG
Cuts at: 0 687 696 1071 1086 1254 1383
Size: 687 9 375 15 168 129
Fragments arranged by size:
687 375 168 129 15 9
NaeI GCC'GGC
Cuts at: 0 776 1383
Size: 776 607
NcoI C'CATG_G
Cuts at: 0 509 1383
Size: 509 874
NgoMIV G'CCGG_C
Cuts at: 0 774 1383
Size: 774 609
NspI r_CATG'y
Cuts at: 0 1293 1383
Size: 1293 90
PflMI CCAn_nnn'nTGG
Cuts at: 0 472 541 619 1164 1383
Size: 472 69 78 545 219
Fragments arranged by size:
545 472 219 78 69
PmlI CAC'GTG
Cuts at: 0 86 143 1383
Size: 86 57 1240
Fragments arranged by size:
1240 86 57
PpuMI rG'GwC_Cy
Cuts at: 0 21 1383
Size: 21 1362
PspOMI G'GGCC_C
Cuts at: 0 308 367 494 658 722 1280 1383
Size: 308 59 127 164 64 558 103
Fragments arranged by size:
558 308 164 127 103 64 59
PstI C_TGCA'G
Cuts at: 0 686 695 746 896 1070 1085 1383
Size: 686 9 51 150 174 15 298
Fragments arranged by size:
686 298 174 150 51 15 9
PvuII CAG'CTG
Cuts at: 0 687 696 1071 1086 1254 1383
Size: 687 9 375 15 168 129
Fragments arranged by size:
687 375 168 129 15 9
SacI G_AGCT'C
Cuts at: 0 677 1383
Size: 677 706
SbfI CC_TGCA'GG
Cuts at: 0 746 1383
Size: 746 637
SexAI A'CCwGG_T
Cuts at: 0 713 1383
Size: 713 670
SfcI C'TryA_G
Cuts at: 0 682 691 742 892 1066 1081 1383
Size: 682 9 51 150 174 15 302
Fragments arranged by size:
682 302 174 150 51 15 9
SmlI C'TyrA_G
Cuts at: 0 1111 1383
Size: 1111 272
SphI G_CATG'C
Cuts at: 0 1293 1383
Size: 1293 90
StyI C'CwwG_G
Cuts at: 0 446 509 1298 1383
Size: 446 63 789 85
Fragments arranged by size:
789 446 85 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1027 1383
Size: 1027 356
TatI w'GTAC_w
Cuts at: 0 293 1383
Size: 293 1090
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 19 51 1383
Size: 19 32 1332
Fragments arranged by size:
1332 32 19
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AlfI AloI
AlwNI ApaI ApaLI ApoI BanI BanII BbsI BcgI
BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI
BsaWI BsaXI BseYI BsgI BsiHKAI BsiWI Bsp1286I BspMI
BsrFI BsrGI BssHII BstAPI BstEII BstXI BtgI BtsI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PflMI PmlI PpuMI PspOMI PstI PvuII SacI
SbfI SexAI SfcI SmlI SphI StyI TaqII TatI
TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI BsaI BsaBI
BsaHI BseRI BsiEI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BssSI BstBI BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EagI EciI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NarI NdeI NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI