Restriction map for
pF1KB7695
(Linear) (Six-base) MAPSORT of: KIBB7695.seq Check: 254 from: 1 to: 1161
KIBB7695.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 415 1147 1161
Size: 334 81 732 14
Fragments arranged by size:
732 334 81 14
AflIII A'CryG_T
Cuts at: 0 212 1052 1161
Size: 212 840 109
Fragments arranged by size:
840 212 109
AhdI GACnn_n'nnGTC
Cuts at: 0 854 1161
Size: 854 307
AlwNI CAG_nnn'CTG
Cuts at: 0 281 438 1020 1161
Size: 281 157 582 141
Fragments arranged by size:
582 281 157 141
AvaI C'yCGr_G
Cuts at: 0 198 349 457 859 1161
Size: 198 151 108 402 302
Fragments arranged by size:
402 302 198 151 108
AvrII C'CTAG_G
Cuts at: 0 30 1161
Size: 30 1131
BanI G'GyrC_C
Cuts at: 0 786 1121 1161
Size: 786 335 40
Fragments arranged by size:
786 335 40
BanII G_rGCy'C
Cuts at: 0 629 841 1085 1161
Size: 629 212 244 76
Fragments arranged by size:
629 244 212 76
BbeI G_GCGC'C
Cuts at: 0 790 1161
Size: 790 371
BbsI GAAGACnn'nnnn_
Cuts at: 0 178 473 853 1161
Size: 178 295 380 308
Fragments arranged by size:
380 308 295 178
BglI GCCn_nnn'nGGC
Cuts at: 0 784 836 1161
Size: 784 52 325
Fragments arranged by size:
784 325 52
BlpI GC'TnA_GC
Cuts at: 0 628 1161
Size: 628 533
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 460 583 1119 1161
Size: 280 180 123 536 42
Fragments arranged by size:
536 280 180 123 42
Bpu10I CC'TnA_GC
Cuts at: 0 25 1161
Size: 25 1136
BsaI GGTCTCn'nnnn_
Cuts at: 0 400 538 1161
Size: 400 138 623
Fragments arranged by size:
623 400 138
BsaAI yAC'GTr
Cuts at: 0 215 1161
Size: 215 946
BsaHI Gr'CG_yC
Cuts at: 0 787 1161
Size: 787 374
BsaWI w'CCGG_w
Cuts at: 0 536 707 1161
Size: 536 171 454
Fragments arranged by size:
536 454 171
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 856 886 1161
Size: 856 30 275
Fragments arranged by size:
856 275 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 415 418 676 679 688 1161
Size: 415 3 258 3 9 473
Fragments arranged by size:
473 415 258 9 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 1161
Size: 386 775
BsiEI CG_ry'CG
Cuts at: 0 622 1041 1161
Size: 622 419 120
Fragments arranged by size:
622 419 120
BsiHKAI G_wGCw'C
Cuts at: 0 65 1161
Size: 65 1096
BsmBI CGTCTCn'nnnn_
Cuts at: 0 364 1161
Size: 364 797
Bsp1286I G_dGCh'C
Cuts at: 0 65 629 841 1085 1161
Size: 65 564 212 244 76
Fragments arranged by size:
564 244 212 76 65
BspEI T'CCGG_A
Cuts at: 0 536 1161
Size: 536 625
BsrFI r'CCGG_y
Cuts at: 0 133 342 617 1161
Size: 133 209 275 544
Fragments arranged by size:
544 275 209 133
BssHII G'CGCG_C
Cuts at: 0 82 1161
Size: 82 1079
BssSI C'ACGA_G
Cuts at: 0 906 1161
Size: 906 255
BstAPI GCAn_nnn'nTGC
Cuts at: 0 971 980 989 1161
Size: 971 9 9 172
Fragments arranged by size:
971 172 9 9
BstEII G'GTnAC_C
Cuts at: 0 1045 1161
Size: 1045 116
Bsu36I CC'TnA_GG
Cuts at: 0 755 1161
Size: 755 406
BtgI C'CryG_G
Cuts at: 0 180 1073 1161
Size: 180 893 88
Fragments arranged by size:
893 180 88
BtsI GCAGTG_nn'
Cuts at: 0 63 967 1161
Size: 63 904 194
Fragments arranged by size:
904 194 63
DrdI GACnn_nn'nnGTC
Cuts at: 0 799 1161
Size: 799 362
EaeI y'GGCC_r
Cuts at: 0 2 619 656 1161
Size: 2 617 37 505
Fragments arranged by size:
617 505 37 2
EagI C'GGCC_G
Cuts at: 0 619 1161
Size: 619 542
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1093 1161
Size: 1093 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 334 415 460 583 1119 1147 1161
Size: 280 54 81 45 123 536 28 14
Fragments arranged by size:
536 280 123 81 54 45 28 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 34 316 658 1161
Size: 34 282 342 503
Fragments arranged by size:
503 342 282 34
EcoO109I rG'GnC_Cy
Cuts at: 0 264 278 750 864 1161
Size: 264 14 472 114 297
Fragments arranged by size:
472 297 264 114 14
EcoRV GAT'ATC
Cuts at: 0 594 1161
Size: 594 567
HaeII r_GCGC'y
Cuts at: 0 790 1161
Size: 790 371
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 239 271 840 856 872 888 961 993
Size: 239 32 569 16 16 16 73 32
Cuts at: 993 1161
Size: 168
Fragments arranged by size:
569 239 168 73 32 32 16 16
16
KasI G'GCGC_C
Cuts at: 0 786 1161
Size: 786 375
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 1161
Size: 211 950
MscI TGG'CCA
Cuts at: 0 4 1161
Size: 4 1157
MslI CAynn'nnrTG
Cuts at: 0 163 1161
Size: 163 998
MspA1I CmG'CkG
Cuts at: 0 182 227 956 1017 1161
Size: 182 45 729 61 144
Fragments arranged by size:
729 182 144 61 45
NarI GG'CG_CC
Cuts at: 0 787 1161
Size: 787 374
NcoI C'CATG_G
Cuts at: 0 1073 1161
Size: 1073 88
NdeI CA'TA_TG
Cuts at: 0 519 1161
Size: 519 642
NspI r_CATG'y
Cuts at: 0 1056 1161
Size: 1056 105
PciI A'CATG_T
Cuts at: 0 1052 1161
Size: 1052 109
PfoI T'CCnGG_A
Cuts at: 0 257 332 1161
Size: 257 75 829
Fragments arranged by size:
829 257 75
PpuMI rG'GwC_Cy
Cuts at: 0 264 750 864 1161
Size: 264 486 114 297
Fragments arranged by size:
486 297 264 114
PspXI vC'TCGA_Gb
Cuts at: 0 349 457 1161
Size: 349 108 704
Fragments arranged by size:
704 349 108
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 139 171 1161
Size: 139 32 990
Fragments arranged by size:
990 139 32
PstI C_TGCA'G
Cuts at: 0 985 1161
Size: 985 176
PvuI CG_AT'CG
Cuts at: 0 1041 1161
Size: 1041 120
PvuII CAG'CTG
Cuts at: 0 1017 1161
Size: 1017 144
SacII CC_GC'GG
Cuts at: 0 183 1161
Size: 183 978
SanDI GG'GwC_CC
Cuts at: 0 750 1161
Size: 750 411
SfcI C'TryA_G
Cuts at: 0 981 1161
Size: 981 180
SfoI GGC'GCC
Cuts at: 0 788 1161
Size: 788 373
SmaI CCC'GGG
Cuts at: 0 200 1161
Size: 200 961
SmlI C'TyrA_G
Cuts at: 0 349 457 1161
Size: 349 108 704
Fragments arranged by size:
704 349 108
StyI C'CwwG_G
Cuts at: 0 30 1073 1161
Size: 30 1043 88
Fragments arranged by size:
1043 88 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 584 658 664 1161
Size: 584 74 6 497
Fragments arranged by size:
584 497 74 6
TatI w'GTAC_w
Cuts at: 0 287 1055 1161
Size: 287 768 106
Fragments arranged by size:
768 287 106
XhoI C'TCGA_G
Cuts at: 0 349 457 1161
Size: 349 108 704
Fragments arranged by size:
704 349 108
XmaI C'CCGG_G
Cuts at: 0 198 1161
Size: 198 963
Enzymes that do cut:
AcuI AflIII AhdI AlwNI AvaI AvrII BanI BanII
BbeI BbsI BglI BlpI BpmI Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BsrFI BssHII BssSI BstAPI BstEII Bsu36I
BtgI BtsI DrdI EaeI EagI EciI Eco57MI EcoNI
EcoO109I EcoRV HaeII Hin4I KasI MmeI MscI MslI
MspA1I NarI NcoI NdeI NspI PciI PfoI PpuMI
PspXI PsrI PstI PvuI PvuII SacII SanDI SfcI
SfoI SmaI SmlI StyI TaqII TatI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglII Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaBI
BseYI BsiWI BsmI BspHI BspMI BsrBI BsrDI BsrGI
BstBI BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI
DraIII EarI EcoICRI EcoRI FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NaeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XmnI ZraI