Restriction map for
pF1KB7691
(Linear) (Six-base) MAPSORT of: KIBB7691.seq Check: 3582 from: 1 to: 1047
KIBB7691.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 1047
Size: 240 807
AleI CACnn'nnGTG
Cuts at: 0 547 821 1047
Size: 547 274 226
Fragments arranged by size:
547 274 226
AlwNI CAG_nnn'CTG
Cuts at: 0 930 1047
Size: 930 117
ApoI r'AATT_y
Cuts at: 0 355 1047
Size: 355 692
BanII G_rGCy'C
Cuts at: 0 485 961 1047
Size: 485 476 86
Fragments arranged by size:
485 476 86
BbsI GAAGACnn'nnnn_
Cuts at: 0 544 1047
Size: 544 503
Bme1580I G_kGCm'C
Cuts at: 0 285 1047
Size: 285 762
BmgBI CAC'GTC
Cuts at: 0 519 1047
Size: 519 528
BmrI ACTGGGnnnn_n'
Cuts at: 0 835 1047
Size: 835 212
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 113 504 1047
Size: 113 391 543
Fragments arranged by size:
543 391 113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 1047
Size: 721 326
BsaI GGTCTCn'nnnn_
Cuts at: 0 382 1047
Size: 382 665
BsaWI w'CCGG_w
Cuts at: 0 99 1047
Size: 99 948
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 771 801 1047
Size: 771 30 246
Fragments arranged by size:
771 246 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 264 562 1047
Size: 264 298 485
Fragments arranged by size:
485 298 264
Bsp1286I G_dGCh'C
Cuts at: 0 285 485 961 1047
Size: 285 200 476 86
Fragments arranged by size:
476 285 200 86
BspEI T'CCGG_A
Cuts at: 0 99 1047
Size: 99 948
BspHI T'CATG_A
Cuts at: 0 626 722 1047
Size: 626 96 325
Fragments arranged by size:
626 325 96
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 601 1047
Size: 601 446
BsrBI CCG'CTC
Cuts at: 0 287 438 1047
Size: 287 151 609
Fragments arranged by size:
609 287 151
BsrGI T'GTAC_A
Cuts at: 0 166 1047
Size: 166 881
BstAPI GCAn_nnn'nTGC
Cuts at: 0 585 1047
Size: 585 462
BstYI r'GATC_y
Cuts at: 0 15 1047
Size: 15 1032
BtgI C'CryG_G
Cuts at: 0 610 791 1047
Size: 610 181 256
Fragments arranged by size:
610 256 181
BtsI GCAGTG_nn'
Cuts at: 0 463 981 1047
Size: 463 518 66
Fragments arranged by size:
518 463 66
DraIII CAC_nnn'GTG
Cuts at: 0 402 1047
Size: 402 645
EaeI y'GGCC_r
Cuts at: 0 460 613 1047
Size: 460 153 434
Fragments arranged by size:
460 434 153
EarI CTCTTCn'nnn_
Cuts at: 0 385 1047
Size: 385 662
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 480 1047
Size: 480 567
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 721 1047
Size: 240 481 326
Fragments arranged by size:
481 326 240
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 285 1047
Size: 285 762
HaeII r_GCGC'y
Cuts at: 0 428 1047
Size: 428 619
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 60 92 197 229 402 434 771 803
Size: 60 32 105 32 173 32 337 32
Cuts at: 803 1047
Size: 244
Fragments arranged by size:
337 244 173 105 60 32 32 32
32
MslI CAynn'nnrTG
Cuts at: 0 547 724 821 1047
Size: 547 177 97 226
Fragments arranged by size:
547 226 177 97
MspA1I CmG'CkG
Cuts at: 0 317 478 576 612 1047
Size: 317 161 98 36 435
Fragments arranged by size:
435 317 161 98 36
NcoI C'CATG_G
Cuts at: 0 791 1047
Size: 791 256
NspI r_CATG'y
Cuts at: 0 118 125 173 1047
Size: 118 7 48 874
Fragments arranged by size:
874 118 48 7
PasI CC'CwG_GG
Cuts at: 0 346 1047
Size: 346 701
PflMI CCAn_nnn'nTGG
Cuts at: 0 402 1047
Size: 402 645
PstI C_TGCA'G
Cuts at: 0 331 1047
Size: 331 716
PvuII CAG'CTG
Cuts at: 0 576 1047
Size: 576 471
SacII CC_GC'GG
Cuts at: 0 613 1047
Size: 613 434
ScaI AGT'ACT
Cuts at: 0 878 1047
Size: 878 169
SfcI C'TryA_G
Cuts at: 0 327 1047
Size: 327 720
SphI G_CATG'C
Cuts at: 0 118 1047
Size: 118 929
StyI C'CwwG_G
Cuts at: 0 290 791 868 1047
Size: 290 501 77 179
Fragments arranged by size:
501 290 179 77
TatI w'GTAC_w
Cuts at: 0 166 849 876 1047
Size: 166 683 27 171
Fragments arranged by size:
683 171 166 27
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 343 1047
Size: 343 704
XmnI GAAnn'nnTTC
Cuts at: 0 268 1047
Size: 268 779
Enzymes that do cut:
AcuI AleI AlwNI ApoI BanII BbsI Bme1580I BmgBI
BmrI BplI BpmI BsaI BsaWI BsaXI BsgI Bsp1286I
BspEI BspHI BspMI BsrBI BsrGI BstAPI BstYI BtgI
BtsI DraIII EaeI EarI EciI Eco57MI FalI HaeII
Hin4I MslI MspA1I NcoI NspI PasI PflMI PstI
PvuII SacII ScaI SfcI SphI StyI TatI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmtI
Bpu10I BpuEI BsaAI BsaBI BsaHI BseRI BseYI BsiEI
BsiHKAI BsiWI BsmI BsmBI BsrDI BsrFI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XhoI XmaI ZraI