Restriction map for
pF1KB7675
(Linear) (Six-base) MAPSORT of: KIBB7675.seq Check: 6286 from: 1 to: 1023
KIBB7675.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AfeI AGC'GCT
Cuts at: 0 458 1023
Size: 458 565
AleI CACnn'nnGTG
Cuts at: 0 247 941 1023
Size: 247 694 82
Fragments arranged by size:
694 247 82
AvaI C'yCGr_G
Cuts at: 0 227 529 1023
Size: 227 302 494
Fragments arranged by size:
494 302 227
BanI G'GyrC_C
Cuts at: 0 516 534 771 919 1023
Size: 516 18 237 148 104
Fragments arranged by size:
516 237 148 104 18
BanII G_rGCy'C
Cuts at: 0 193 364 744 1023
Size: 193 171 380 279
Fragments arranged by size:
380 279 193 171
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 372 406 1023
Size: 372 34 617
Fragments arranged by size:
617 372 34
Bme1580I G_kGCm'C
Cuts at: 0 353 521 922 1023
Size: 353 168 401 101
Fragments arranged by size:
401 353 168 101
BmrI ACTGGGnnnn_n'
Cuts at: 0 112 1023
Size: 112 911
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 207 1023
Size: 207 816
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 1023
Size: 766 257
BsaI GGTCTCn'nnnn_
Cuts at: 0 147 272 787 1023
Size: 147 125 515 236
Fragments arranged by size:
515 236 147 125
BsaHI Gr'CG_yC
Cuts at: 0 445 1023
Size: 445 578
BsaWI w'CCGG_w
Cuts at: 0 260 1023
Size: 260 763
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 29 59 1023
Size: 29 30 964
Fragments arranged by size:
964 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 50 570 682 1023
Size: 50 520 112 341
Fragments arranged by size:
520 341 112 50
BseYI C'CCAG_C
Cuts at: 0 30 404 487 786 837 913 1023
Size: 30 374 83 299 51 76 110
Fragments arranged by size:
374 299 110 83 76 51 30
BsiEI CG_ry'CG
Cuts at: 0 115 509 1023
Size: 115 394 514
Fragments arranged by size:
514 394 115
BsiHKAI G_wGCw'C
Cuts at: 0 364 1023
Size: 364 659
BsmI GAATG_Cn'
Cuts at: 0 374 980 1023
Size: 374 606 43
Fragments arranged by size:
606 374 43
BsmBI CGTCTCn'nnnn_
Cuts at: 0 178 1023
Size: 178 845
Bsp1286I G_dGCh'C
Cuts at: 0 193 353 364 521 744 922 1023
Size: 193 160 11 157 223 178 101
Fragments arranged by size:
223 193 178 160 157 101 11
BspEI T'CCGG_A
Cuts at: 0 260 1023
Size: 260 763
BsrBI CCG'CTC
Cuts at: 0 614 1023
Size: 614 409
BstYI r'GATC_y
Cuts at: 0 164 302 1023
Size: 164 138 721
Fragments arranged by size:
721 164 138
BtgI C'CryG_G
Cuts at: 0 245 1023
Size: 245 778
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 941 1023
Size: 941 82
EaeI y'GGCC_r
Cuts at: 0 506 557 1023
Size: 506 51 466
Fragments arranged by size:
506 466 51
EagI C'GGCC_G
Cuts at: 0 506 1023
Size: 506 517
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 337 1023
Size: 337 686
EcoICRI GAG'CTC
Cuts at: 0 362 1023
Size: 362 661
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 1023
Size: 766 257
EcoNI CCTnn'n_nnAGG
Cuts at: 0 748 1023
Size: 748 275
FspI TGC'GCA
Cuts at: 0 382 1023
Size: 382 641
HaeII r_GCGC'y
Cuts at: 0 460 1023
Size: 460 563
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 710 1023
Size: 135 575 313
Fragments arranged by size:
575 313 135
MscI TGG'CCA
Cuts at: 0 559 1023
Size: 559 464
MslI CAynn'nnrTG
Cuts at: 0 247 941 1023
Size: 247 694 82
Fragments arranged by size:
694 247 82
MspA1I CmG'CkG
Cuts at: 0 30 60 790 1023
Size: 30 30 730 233
Fragments arranged by size:
730 233 30 30
NotI GC'GGCC_GC
Cuts at: 0 506 1023
Size: 506 517
NspI r_CATG'y
Cuts at: 0 718 913 1023
Size: 718 195 110
Fragments arranged by size:
718 195 110
PfoI T'CCnGG_A
Cuts at: 0 305 1023
Size: 305 718
PvuII CAG'CTG
Cuts at: 0 30 790 1023
Size: 30 760 233
Fragments arranged by size:
760 233 30
SacI G_AGCT'C
Cuts at: 0 364 1023
Size: 364 659
SfcI C'TryA_G
Cuts at: 0 864 1023
Size: 864 159
SmaI CCC'GGG
Cuts at: 0 229 531 1023
Size: 229 302 492
Fragments arranged by size:
492 302 229
SphI G_CATG'C
Cuts at: 0 718 1023
Size: 718 305
StyI C'CwwG_G
Cuts at: 0 512 659 1023
Size: 512 147 364
Fragments arranged by size:
512 364 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 890 1023
Size: 890 133
TatI w'GTAC_w
Cuts at: 0 341 1023
Size: 341 682
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 29 61 316 348 1023
Size: 29 32 255 32 675
Fragments arranged by size:
675 255 32 32 29
XmaI C'CCGG_G
Cuts at: 0 227 529 1023
Size: 227 302 494
Fragments arranged by size:
494 302 227
Enzymes that do cut:
AfeI AleI AvaI BanI BanII BcgI Bme1580I BmrI
BplI BpmI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BsrBI BstYI
BtgI BtgZI EaeI EagI EciI EcoICRI Eco57MI EcoNI
FspI HaeII MmeI MscI MslI MspA1I NotI NspI
PfoI PvuII SacI SfcI SmaI SphI StyI TaqII
TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI
BbvCI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmtI Bpu10I BpuEI BsaAI BsaBI BsgI BsiWI BspHI
BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI
DraIII DrdI EarI EcoO109I EcoRI EcoRV FalI FseI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI
SspI StuI SwaI Tth111I XbaI XcmI XhoI XmnI
ZraI