(Linear) (Six-base) MAPSORT of: KIBB7666.seq Check: 5012 from: 1 to: 1113 KIBB7666.orf With 182 enzymes: SgfI * January 9, 2015 15:32 .. AflIII A'CryG_T Cuts at: 0 374 1113 Size: 374 739 AhdI GACnn_n'nnGTC Cuts at: 0 159 1113 Size: 159 954 AlwNI CAG_nnn'CTG Cuts at: 0 571 1113 Size: 571 542 AvaI C'yCGr_G Cuts at: 0 324 405 1113 Size: 324 81 708 Fragments arranged by size: 708 324 81 BanII G_rGCy'C Cuts at: 0 245 1113 Size: 245 868 BbsI GAAGACnn'nnnn_ Cuts at: 0 155 1113 Size: 155 958 BbvCI CC'TCA_GC Cuts at: 0 767 1113 Size: 767 346 BglII A'GATC_T Cuts at: 0 107 1113 Size: 107 1006 Bme1580I G_kGCm'C Cuts at: 0 428 1113 Size: 428 685 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 482 548 1113 Size: 482 66 565 Fragments arranged by size: 565 482 66 Bpu10I CC'TnA_GC Cuts at: 0 767 1113 Size: 767 346 BsaAI yAC'GTr Cuts at: 0 759 1113 Size: 759 354 BsaWI w'CCGG_w Cuts at: 0 13 1113 Size: 13 1100 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 32 1113 Size: 32 1081 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 454 914 1113 Size: 454 460 199 Fragments arranged by size: 460 454 199 BsiHKAI G_wGCw'C Cuts at: 0 491 1113 Size: 491 622 BsmI GAATG_Cn' Cuts at: 0 831 1113 Size: 831 282 Bsp1286I G_dGCh'C Cuts at: 0 245 428 491 1113 Size: 245 183 63 622 Fragments arranged by size: 622 245 183 63 BspEI T'CCGG_A Cuts at: 0 13 1113 Size: 13 1100 BssSI C'ACGA_G Cuts at: 0 208 1113 Size: 208 905 BstXI CCAn_nnnn'nTGG Cuts at: 0 682 913 1113 Size: 682 231 200 Fragments arranged by size: 682 231 200 BstYI r'GATC_y Cuts at: 0 107 385 1113 Size: 107 278 728 Fragments arranged by size: 728 278 107 Bsu36I CC'TnA_GG Cuts at: 0 713 1113 Size: 713 400 BtgI C'CryG_G Cuts at: 0 56 428 1113 Size: 56 372 685 Fragments arranged by size: 685 372 56 DraIII CAC_nnn'GTG Cuts at: 0 213 1113 Size: 213 900 EaeI y'GGCC_r Cuts at: 0 194 215 1113 Size: 194 21 898 Fragments arranged by size: 898 194 21 EarI CTCTTCn'nnn_ Cuts at: 0 128 938 1039 1045 1113 Size: 128 810 101 6 68 Fragments arranged by size: 810 128 101 68 6 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 482 548 1113 Size: 482 66 565 Fragments arranged by size: 565 482 66 EcoO109I rG'GnC_Cy Cuts at: 0 39 313 1113 Size: 39 274 800 Fragments arranged by size: 800 274 39 EcoRV GAT'ATC Cuts at: 0 576 1113 Size: 576 537 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 183 1113 Size: 183 930 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 111 143 145 177 520 552 645 677 Size: 111 32 2 32 343 32 93 32 Cuts at: 677 848 880 1113 Size: 171 32 233 Fragments arranged by size: 343 233 171 111 93 32 32 32 32 32 2 HincII GTy'rAC Cuts at: 0 534 1113 Size: 534 579 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 591 1113 Size: 591 522 MscI TGG'CCA Cuts at: 0 217 1113 Size: 217 896 MslI CAynn'nnrTG Cuts at: 0 272 290 346 1113 Size: 272 18 56 767 Fragments arranged by size: 767 272 56 18 MspA1I CmG'CkG Cuts at: 0 1102 1113 Size: 1102 11 NdeI CA'TA_TG Cuts at: 0 650 992 1113 Size: 650 342 121 Fragments arranged by size: 650 342 121 NspI r_CATG'y Cuts at: 0 378 552 931 1113 Size: 378 174 379 182 Fragments arranged by size: 379 378 182 174 PciI A'CATG_T Cuts at: 0 374 1113 Size: 374 739 PflMI CCAn_nnn'nTGG Cuts at: 0 631 1113 Size: 631 482 PfoI T'CCnGG_A Cuts at: 0 498 1113 Size: 498 615 PmlI CAC'GTG Cuts at: 0 759 1113 Size: 759 354 PpuMI rG'GwC_Cy Cuts at: 0 39 313 1113 Size: 39 274 800 Fragments arranged by size: 800 274 39 PspXI vC'TCGA_Gb Cuts at: 0 324 1113 Size: 324 789 PvuII CAG'CTG Cuts at: 0 1102 1113 Size: 1102 11 SanDI GG'GwC_CC Cuts at: 0 39 313 1113 Size: 39 274 800 Fragments arranged by size: 800 274 39 SapI GCTCTTCn'nnn_ Cuts at: 0 938 1113 Size: 938 175 SfcI C'TryA_G Cuts at: 0 1063 1113 Size: 1063 50 SmlI C'TyrA_G Cuts at: 0 324 1113 Size: 324 789 SphI G_CATG'C Cuts at: 0 931 1113 Size: 931 182 SspI AAT'ATT Cuts at: 0 958 1113 Size: 958 155 StuI AGG'CCT Cuts at: 0 299 1113 Size: 299 814 StyI C'CwwG_G Cuts at: 0 167 1113 Size: 167 946 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 414 1113 Size: 414 699 TatI w'GTAC_w Cuts at: 0 877 1113 Size: 877 236 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 197 229 1113 Size: 197 32 884 Fragments arranged by size: 884 197 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 632 1113 Size: 632 481 XhoI C'TCGA_G Cuts at: 0 324 1113 Size: 324 789 Enzymes that do cut: AflIII AhdI AlwNI AvaI BanII BbsI BbvCI BglII Bme1580I BpmI Bpu10I BsaAI BsaWI BseRI BsgI BsiHKAI BsmI Bsp1286I BspEI BssSI BstXI BstYI Bsu36I BtgI DraIII EaeI EarI Eco57MI EcoO109I EcoRV FalI Hin4I HincII MmeI MscI MslI MspA1I NdeI NspI PciI PflMI PfoI PmlI PpuMI PspXI PvuII SanDI SapI SfcI SmlI SphI SspI StuI StyI TaqII TatI TstI XcmI XhoI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI BglI BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI BsaHI BsaXI BseYI BsiEI BsiWI BsmBI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I XbaI XmaI XmnI ZraI