Restriction map for pF1KB7666
 (Linear) (Six-base) MAPSORT of: KIBB7666.seq  Check: 5012  from: 1  to: 1113



KIBB7666.orf

 With 182 enzymes: SgfI * 

                        January 9, 2015 15:32  ..

AflIII A'CryG_T

Cuts at:      0     374    1113 
   Size:        374     739
 
AhdI GACnn_n'nnGTC

Cuts at:      0     159    1113 
   Size:        159     954
 
AlwNI CAG_nnn'CTG

Cuts at:      0     571    1113 
   Size:        571     542
 
AvaI C'yCGr_G

Cuts at:      0     324     405    1113 
   Size:        324      81     708

  Fragments arranged by size: 

                 708     324      81
 
BanII G_rGCy'C

Cuts at:      0     245    1113 
   Size:        245     868
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     155    1113 
   Size:        155     958
 
BbvCI CC'TCA_GC

Cuts at:      0     767    1113 
   Size:        767     346
 
BglII A'GATC_T

Cuts at:      0     107    1113 
   Size:        107    1006
 
Bme1580I G_kGCm'C

Cuts at:      0     428    1113 
   Size:        428     685
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     482     548    1113 
   Size:        482      66     565

  Fragments arranged by size: 

                 565     482      66
 
Bpu10I CC'TnA_GC

Cuts at:      0     767    1113 
   Size:        767     346
 
BsaAI yAC'GTr

Cuts at:      0     759    1113 
   Size:        759     354
 
BsaWI w'CCGG_w

Cuts at:      0      13    1113 
   Size:         13    1100
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      32    1113 
   Size:         32    1081
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     454     914    1113 
   Size:        454     460     199

  Fragments arranged by size: 

                 460     454     199
 
BsiHKAI G_wGCw'C

Cuts at:      0     491    1113 
   Size:        491     622
 
BsmI GAATG_Cn'

Cuts at:      0     831    1113 
   Size:        831     282
 
Bsp1286I G_dGCh'C

Cuts at:      0     245     428     491    1113 
   Size:        245     183      63     622

  Fragments arranged by size: 

                 622     245     183      63
 
BspEI T'CCGG_A

Cuts at:      0      13    1113 
   Size:         13    1100
 
BssSI C'ACGA_G

Cuts at:      0     208    1113 
   Size:        208     905
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     682     913    1113 
   Size:        682     231     200

  Fragments arranged by size: 

                 682     231     200
 
BstYI r'GATC_y

Cuts at:      0     107     385    1113 
   Size:        107     278     728

  Fragments arranged by size: 

                 728     278     107
 
Bsu36I CC'TnA_GG

Cuts at:      0     713    1113 
   Size:        713     400
 
BtgI C'CryG_G

Cuts at:      0      56     428    1113 
   Size:         56     372     685

  Fragments arranged by size: 

                 685     372      56
 
DraIII CAC_nnn'GTG

Cuts at:      0     213    1113 
   Size:        213     900
 
EaeI y'GGCC_r

Cuts at:      0     194     215    1113 
   Size:        194      21     898

  Fragments arranged by size: 

                 898     194      21
 
EarI CTCTTCn'nnn_

Cuts at:      0     128     938    1039    1045    1113 
   Size:        128     810     101       6      68

  Fragments arranged by size: 

                 810     128     101      68       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     482     548    1113 
   Size:        482      66     565

  Fragments arranged by size: 

                 565     482      66
 
EcoO109I rG'GnC_Cy

Cuts at:      0      39     313    1113 
   Size:         39     274     800

  Fragments arranged by size: 

                 800     274      39
 
EcoRV GAT'ATC

Cuts at:      0     576    1113 
   Size:        576     537
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     183    1113 
   Size:        183     930
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     111     143     145     177     520     552     645     677 
   Size:        111      32       2      32     343      32      93      32

Cuts at:    677     848     880    1113 
   Size:        171      32     233

  Fragments arranged by size: 

                 343     233     171     111      93      32      32      32
                  32      32       2
 
HincII GTy'rAC

Cuts at:      0     534    1113 
   Size:        534     579
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     591    1113 
   Size:        591     522
 
MscI TGG'CCA

Cuts at:      0     217    1113 
   Size:        217     896
 
MslI CAynn'nnrTG

Cuts at:      0     272     290     346    1113 
   Size:        272      18      56     767

  Fragments arranged by size: 

                 767     272      56      18
 
MspA1I CmG'CkG

Cuts at:      0    1102    1113 
   Size:       1102      11
 
NdeI CA'TA_TG

Cuts at:      0     650     992    1113 
   Size:        650     342     121

  Fragments arranged by size: 

                 650     342     121
 
NspI r_CATG'y

Cuts at:      0     378     552     931    1113 
   Size:        378     174     379     182

  Fragments arranged by size: 

                 379     378     182     174
 
PciI A'CATG_T

Cuts at:      0     374    1113 
   Size:        374     739
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     631    1113 
   Size:        631     482
 
PfoI T'CCnGG_A

Cuts at:      0     498    1113 
   Size:        498     615
 
PmlI CAC'GTG

Cuts at:      0     759    1113 
   Size:        759     354
 
PpuMI rG'GwC_Cy

Cuts at:      0      39     313    1113 
   Size:         39     274     800

  Fragments arranged by size: 

                 800     274      39
 
PspXI vC'TCGA_Gb

Cuts at:      0     324    1113 
   Size:        324     789
 
PvuII CAG'CTG

Cuts at:      0    1102    1113 
   Size:       1102      11
 
SanDI GG'GwC_CC

Cuts at:      0      39     313    1113 
   Size:         39     274     800

  Fragments arranged by size: 

                 800     274      39
 
SapI GCTCTTCn'nnn_

Cuts at:      0     938    1113 
   Size:        938     175
 
SfcI C'TryA_G

Cuts at:      0    1063    1113 
   Size:       1063      50
 
SmlI C'TyrA_G

Cuts at:      0     324    1113 
   Size:        324     789
 
SphI G_CATG'C

Cuts at:      0     931    1113 
   Size:        931     182
 
SspI AAT'ATT

Cuts at:      0     958    1113 
   Size:        958     155
 
StuI AGG'CCT

Cuts at:      0     299    1113 
   Size:        299     814
 
StyI C'CwwG_G

Cuts at:      0     167    1113 
   Size:        167     946
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     414    1113 
   Size:        414     699
 
TatI w'GTAC_w

Cuts at:      0     877    1113 
   Size:        877     236
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     197     229    1113 
   Size:        197      32     884

  Fragments arranged by size: 

                 884     197      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     632    1113 
   Size:        632     481
 
XhoI C'TCGA_G

Cuts at:      0     324    1113 
   Size:        324     789
 

 Enzymes that do cut:

   AflIII     AhdI    AlwNI     AvaI    BanII     BbsI    BbvCI    BglII
 Bme1580I     BpmI   Bpu10I    BsaAI    BsaWI    BseRI     BsgI  BsiHKAI
     BsmI Bsp1286I    BspEI    BssSI    BstXI    BstYI   Bsu36I     BtgI
   DraIII     EaeI     EarI  Eco57MI EcoO109I    EcoRV     FalI    Hin4I
   HincII     MmeI     MscI     MslI   MspA1I     NdeI     NspI     PciI
    PflMI     PfoI     PmlI    PpuMI    PspXI    PvuII    SanDI     SapI
     SfcI     SmlI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI
     BplI    BpuEI     BsaI    BsaBI    BsaHI    BsaXI    BseYI    BsiEI
    BsiWI    BsmBI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PmeI     PpiI    PshAI     PsiI
   PspOMI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SwaI  Tth111I     XbaI     XmaI     XmnI
     ZraI
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