Restriction map for
pF1KB7664
(Linear) (Six-base) MAPSORT of: KIBB7664.seq Check: 3741 from: 1 to: 1188
KIBB7664.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 0 852 1188
Size: 852 336
AleI CACnn'nnGTG
Cuts at: 0 146 1188
Size: 146 1042
AlwNI CAG_nnn'CTG
Cuts at: 0 513 1188
Size: 513 675
ApoI r'AATT_y
Cuts at: 0 1075 1188
Size: 1075 113
AvaI C'yCGr_G
Cuts at: 0 186 209 1188
Size: 186 23 979
Fragments arranged by size:
979 186 23
BanI G'GyrC_C
Cuts at: 0 651 1188
Size: 651 537
BanII G_rGCy'C
Cuts at: 0 210 746 1188
Size: 210 536 442
Fragments arranged by size:
536 442 210
BbsI GAAGACnn'nnnn_
Cuts at: 0 48 1188
Size: 48 1140
BciVI GTATCCnnnnn_n'
Cuts at: 0 769 1188
Size: 769 419
BclI T'GATC_A
Cuts at: 0 882 1188
Size: 882 306
Bme1580I G_kGCm'C
Cuts at: 0 656 693 1188
Size: 656 37 495
Fragments arranged by size:
656 495 37
BmgBI CAC'GTC
Cuts at: 0 60 1188
Size: 60 1128
BmrI ACTGGGnnnn_n'
Cuts at: 0 856 1188
Size: 856 332
Bpu10I CC'TnA_GC
Cuts at: 0 521 996 1188
Size: 521 475 192
Fragments arranged by size:
521 475 192
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1188
Size: 718 470
BsaAI yAC'GTr
Cuts at: 0 757 1188
Size: 757 431
BsaHI Gr'CG_yC
Cuts at: 0 777 1188
Size: 777 411
BsaWI w'CCGG_w
Cuts at: 0 135 145 1188
Size: 135 10 1043
Fragments arranged by size:
1043 135 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 227 263 1174 1177 1180 1188
Size: 227 36 911 3 3 8
Fragments arranged by size:
911 227 36 8 3 3
BsmBI CGTCTCn'nnnn_
Cuts at: 0 103 164 784 1188
Size: 103 61 620 404
Fragments arranged by size:
620 404 103 61
Bsp1286I G_dGCh'C
Cuts at: 0 210 656 693 746 1188
Size: 210 446 37 53 442
Fragments arranged by size:
446 442 210 53 37
BspEI T'CCGG_A
Cuts at: 0 135 1188
Size: 135 1053
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 521 1188
Size: 521 667
BsrBI CCG'CTC
Cuts at: 0 185 534 1188
Size: 185 349 654
Fragments arranged by size:
654 349 185
BsrDI GCAATG_nn'
Cuts at: 0 863 1008 1188
Size: 863 145 180
Fragments arranged by size:
863 180 145
BsrFI r'CCGG_y
Cuts at: 0 35 69 177 221 1188
Size: 35 34 108 44 967
Fragments arranged by size:
967 108 44 35 34
BsrGI T'GTAC_A
Cuts at: 0 818 1188
Size: 818 370
BstXI CCAn_nnnn'nTGG
Cuts at: 0 148 1188
Size: 148 1040
Bsu36I CC'TnA_GG
Cuts at: 0 252 1188
Size: 252 936
BtgI C'CryG_G
Cuts at: 0 195 1188
Size: 195 993
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 550 1188
Size: 550 638
BtsI GCAGTG_nn'
Cuts at: 0 603 1188
Size: 603 585
DraI TTT'AAA
Cuts at: 0 792 1188
Size: 792 396
EaeI y'GGCC_r
Cuts at: 0 960 1188
Size: 960 228
EarI CTCTTCn'nnn_
Cuts at: 0 7 524 960 1188
Size: 7 517 436 228
Fragments arranged by size:
517 436 228 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 152 1188
Size: 152 1036
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 874 906 979 1011 1085 1117 1188
Size: 874 32 73 32 74 32 71
Fragments arranged by size:
874 74 73 71 32 32 32
HincII GTy'rAC
Cuts at: 0 853 1188
Size: 853 335
HindIII A'AGCT_T
Cuts at: 0 672 1188
Size: 672 516
MfeI C'AATT_G
Cuts at: 0 878 1188
Size: 878 310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1100 1188
Size: 1100 88
MslI CAynn'nnrTG
Cuts at: 0 146 1188
Size: 146 1042
MspA1I CmG'CkG
Cuts at: 0 197 1188
Size: 197 991
NaeI GCC'GGC
Cuts at: 0 37 71 179 1188
Size: 37 34 108 1009
Fragments arranged by size:
1009 108 37 34
NgoMIV G'CCGG_C
Cuts at: 0 35 69 177 1188
Size: 35 34 108 1011
Fragments arranged by size:
1011 108 35 34
NspI r_CATG'y
Cuts at: 0 910 1188
Size: 910 278
PsiI TTA'TAA
Cuts at: 0 545 1188
Size: 545 643
PstI C_TGCA'G
Cuts at: 0 518 1188
Size: 518 670
SacII CC_GC'GG
Cuts at: 0 198 1188
Size: 198 990
SalI G'TCGA_C
Cuts at: 0 851 1188
Size: 851 337
SapI GCTCTTCn'nnn_
Cuts at: 0 7 524 1188
Size: 7 517 664
Fragments arranged by size:
664 517 7
SbfI CC_TGCA'GG
Cuts at: 0 518 1188
Size: 518 670
SfcI C'TryA_G
Cuts at: 0 514 1188
Size: 514 674
SmlI C'TyrA_G
Cuts at: 0 697 1188
Size: 697 491
SphI G_CATG'C
Cuts at: 0 910 1188
Size: 910 278
StuI AGG'CCT
Cuts at: 0 250 468 1188
Size: 250 218 720
Fragments arranged by size:
720 250 218
StyI C'CwwG_G
Cuts at: 0 990 1188
Size: 990 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 246 1188
Size: 246 942
TatI w'GTAC_w
Cuts at: 0 818 1068 1188
Size: 818 250 120
Fragments arranged by size:
818 250 120
Enzymes that do cut:
AccI AleI AlwNI ApoI AvaI BanI BanII BbsI
BciVI BclI Bme1580I BmgBI BmrI Bpu10I BpuEI BsaAI
BsaHI BsaWI BseRI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BstXI Bsu36I BtgI BtgZI BtsI
DraI EaeI EarI EciI Hin4I HincII HindIII MfeI
MmeI MslI MspA1I NaeI NgoMIV NspI PsiI PstI
SacII SalI SapI SbfI SfcI SmlI SphI StuI
StyI TaqII TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BfrBI
BglI BglII BlpI BmtI BplI BpmI BsaI BsaBI
BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BspHI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I ClaI
CspCI DraIII DrdI EagI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII HpaI
KasI KpnI MluI MscI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SanDI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI