Restriction map for
pF1KB7662
(Linear) (Six-base) MAPSORT of: KIBB7662.seq Check: 3019 from: 1 to: 1188
KIBB7662.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 501 1188
Size: 501 687
AccI GT'mk_AC
Cuts at: 0 279 1188
Size: 279 909
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 1188
Size: 104 1084
AlwNI CAG_nnn'CTG
Cuts at: 0 316 1188
Size: 316 872
ApoI r'AATT_y
Cuts at: 0 522 655 697 915 1188
Size: 522 133 42 218 273
Fragments arranged by size:
522 273 218 133 42
AvaI C'yCGr_G
Cuts at: 0 506 1188
Size: 506 682
BamHI G'GATC_C
Cuts at: 0 5 1188
Size: 5 1183
BanI G'GyrC_C
Cuts at: 0 349 1188
Size: 349 839
BanII G_rGCy'C
Cuts at: 0 131 515 1188
Size: 131 384 673
Fragments arranged by size:
673 384 131
BbsI GAAGACnn'nnnn_
Cuts at: 0 494 1188
Size: 494 694
BbvCI CC'TCA_GC
Cuts at: 0 34 1188
Size: 34 1154
Bme1580I G_kGCm'C
Cuts at: 0 609 1188
Size: 609 579
BmrI ACTGGGnnnn_n'
Cuts at: 0 4 1188
Size: 4 1184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 1188
Size: 319 869
Bpu10I CC'TnA_GC
Cuts at: 0 34 1188
Size: 34 1154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1085 1188
Size: 1085 103
BsaI GGTCTCn'nnnn_
Cuts at: 0 238 1188
Size: 238 950
BsaAI yAC'GTr
Cuts at: 0 222 1188
Size: 222 966
BsaHI Gr'CG_yC
Cuts at: 0 190 1188
Size: 190 998
BsaWI w'CCGG_w
Cuts at: 0 162 1188
Size: 162 1026
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 787 817 1188
Size: 787 30 371
Fragments arranged by size:
787 371 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 129 1188
Size: 129 1059
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 1188
Size: 772 416
BsiEI CG_ry'CG
Cuts at: 0 231 1188
Size: 231 957
Bsp1286I G_dGCh'C
Cuts at: 0 131 515 609 1188
Size: 131 384 94 579
Fragments arranged by size:
579 384 131 94
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 501 1188
Size: 501 687
BsrFI r'CCGG_y
Cuts at: 0 108 1188
Size: 108 1080
BstYI r'GATC_y
Cuts at: 0 5 462 717 1188
Size: 5 457 255 471
Fragments arranged by size:
471 457 255 5
Bsu36I CC'TnA_GG
Cuts at: 0 405 1188
Size: 405 783
DraI TTT'AAA
Cuts at: 0 456 566 1188
Size: 456 110 622
Fragments arranged by size:
622 456 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 33 167 1188
Size: 33 134 1021
Fragments arranged by size:
1021 134 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 319 1188
Size: 104 215 869
Fragments arranged by size:
869 215 104
EcoNI CCTnn'n_nnAGG
Cuts at: 0 328 1188
Size: 328 860
EcoO109I rG'GnC_Cy
Cuts at: 0 313 1188
Size: 313 875
EcoRI G'AATT_C
Cuts at: 0 697 1188
Size: 697 491
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 219 1188
Size: 219 969
HaeII r_GCGC'y
Cuts at: 0 59 998 1188
Size: 59 939 190
Fragments arranged by size:
939 190 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 787 819 894 926 1188
Size: 787 32 75 32 262
Fragments arranged by size:
787 262 75 32 32
HincII GTy'rAC
Cuts at: 0 1089 1188
Size: 1089 99
HpaI GTT'AAC
Cuts at: 0 1089 1188
Size: 1089 99
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 555 1188
Size: 555 633
MspA1I CmG'CkG
Cuts at: 0 150 837 1188
Size: 150 687 351
Fragments arranged by size:
687 351 150
NaeI GCC'GGC
Cuts at: 0 110 1188
Size: 110 1078
NdeI CA'TA_TG
Cuts at: 0 624 1188
Size: 624 564
NgoMIV G'CCGG_C
Cuts at: 0 108 1188
Size: 108 1080
NspI r_CATG'y
Cuts at: 0 1095 1188
Size: 1095 93
PflMI CCAn_nnn'nTGG
Cuts at: 0 316 1188
Size: 316 872
PfoI T'CCnGG_A
Cuts at: 0 315 720 1188
Size: 315 405 468
Fragments arranged by size:
468 405 315
PpuMI rG'GwC_Cy
Cuts at: 0 313 1188
Size: 313 875
PsiI TTA'TAA
Cuts at: 0 1154 1188
Size: 1154 34
PvuI CG_AT'CG
Cuts at: 0 231 1188
Size: 231 957
PvuII CAG'CTG
Cuts at: 0 150 1188
Size: 150 1038
SfcI C'TryA_G
Cuts at: 0 1127 1188
Size: 1127 61
SmlI C'TyrA_G
Cuts at: 0 506 1100 1188
Size: 506 594 88
Fragments arranged by size:
594 506 88
SnaBI TAC'GTA
Cuts at: 0 222 1188
Size: 222 966
StyI C'CwwG_G
Cuts at: 0 47 181 544 799 1188
Size: 47 134 363 255 389
Fragments arranged by size:
389 363 255 134 47
TatI w'GTAC_w
Cuts at: 0 640 1188
Size: 640 548
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 108 140 1188
Size: 108 32 1048
Fragments arranged by size:
1048 108 32
XhoI C'TCGA_G
Cuts at: 0 506 1188
Size: 506 682
XmnI GAAnn'nnTTC
Cuts at: 0 539 1188
Size: 539 649
Enzymes that do cut:
AarI AccI AcuI AlwNI ApoI AvaI BamHI BanI
BanII BbsI BbvCI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BsgI BsiEI
Bsp1286I BspMI BsrFI BstYI Bsu36I DraI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HpaI
MmeI MspA1I NaeI NdeI NgoMIV NspI PflMI PfoI
PpuMI PsiI PvuI PvuII SfcI SmlI SnaBI StyI
TatI TstI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BplI BsaBI BseYI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI
EarI EcoICRI EcoRV FseI FspI FspAI HindIII KasI
KpnI MfeI MluI MscI MslI NarI NcoI NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PshAI PspOMI PspXI PsrI PstI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SpeI SphI SrfI SspI
StuI SwaI TaqII Tth111I XbaI XcmI XmaI ZraI