Restriction map for
pF1KB7658
(Linear) (Six-base) MAPSORT of: KIBB7658.seq Check: 4663 from: 1 to: 1182
KIBB7658.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AccI GT'mk_AC
Cuts at: 0 701 1182
Size: 701 481
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 503 524 1182
Size: 223 280 21 658
Fragments arranged by size:
658 280 223 21
ApaI G_GGCC'C
Cuts at: 0 116 1182
Size: 116 1066
ApoI r'AATT_y
Cuts at: 0 896 954 1182
Size: 896 58 228
Fragments arranged by size:
896 228 58
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 741 774 1182
Size: 741 33 408
Fragments arranged by size:
741 408 33
BamHI G'GATC_C
Cuts at: 0 399 1182
Size: 399 783
BanII G_rGCy'C
Cuts at: 0 116 232 1182
Size: 116 116 950
Fragments arranged by size:
950 116 116
BbsI GAAGACnn'nnnn_
Cuts at: 0 530 1182
Size: 530 652
Bme1580I G_kGCm'C
Cuts at: 0 116 1182
Size: 116 1066
BmgBI CAC'GTC
Cuts at: 0 419 1182
Size: 419 763
BmrI ACTGGGnnnn_n'
Cuts at: 0 179 1182
Size: 179 1003
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 705 1182
Size: 705 477
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 842 1182
Size: 160 682 340
Fragments arranged by size:
682 340 160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 1182
Size: 216 966
BsaI GGTCTCn'nnnn_
Cuts at: 0 196 576 1074 1182
Size: 196 380 498 108
Fragments arranged by size:
498 380 196 108
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 352 665 1057 1182
Size: 352 313 392 125
Fragments arranged by size:
392 352 313 125
BseYI C'CCAG_C
Cuts at: 0 78 1182
Size: 78 1104
BsiHKAI G_wGCw'C
Cuts at: 0 146 232 1182
Size: 146 86 950
Fragments arranged by size:
950 146 86
Bsp1286I G_dGCh'C
Cuts at: 0 116 146 232 1182
Size: 116 30 86 950
Fragments arranged by size:
950 116 86 30
BspHI T'CATG_A
Cuts at: 0 972 1068 1182
Size: 972 96 114
Fragments arranged by size:
972 114 96
BstYI r'GATC_y
Cuts at: 0 399 492 1182
Size: 399 93 690
Fragments arranged by size:
690 399 93
Bsu36I CC'TnA_GG
Cuts at: 0 335 1182
Size: 335 847
BtsI GCAGTG_nn'
Cuts at: 0 372 447 1182
Size: 372 75 735
Fragments arranged by size:
735 372 75
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1083 1118 1182
Size: 1083 35 64
Fragments arranged by size:
1083 64 35
DraIII CAC_nnn'GTG
Cuts at: 0 766 1182
Size: 766 416
DrdI GACnn_nn'nnGTC
Cuts at: 0 442 1182
Size: 442 740
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 53 1182
Size: 53 1129
EcoICRI GAG'CTC
Cuts at: 0 230 1182
Size: 230 952
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 223 503 524 842 1182
Size: 160 63 280 21 318 340
Fragments arranged by size:
340 318 280 160 63 21
EcoO109I rG'GnC_Cy
Cuts at: 0 812 1182
Size: 812 370
EcoRI G'AATT_C
Cuts at: 0 896 954 1182
Size: 896 58 228
Fragments arranged by size:
896 228 58
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 63 109 222 1182
Size: 63 46 113 960
Fragments arranged by size:
960 113 63 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 214 246 295 297 327 329 384 416
Size: 214 32 49 2 30 2 55 32
Cuts at: 416 1182
Size: 766
Fragments arranged by size:
766 214 55 49 32 32 30 2
2
HindIII A'AGCT_T
Cuts at: 0 271 858 1182
Size: 271 587 324
Fragments arranged by size:
587 324 271
NdeI CA'TA_TG
Cuts at: 0 1086 1182
Size: 1086 96
PflMI CCAn_nnn'nTGG
Cuts at: 0 766 1018 1182
Size: 766 252 164
Fragments arranged by size:
766 252 164
PpuMI rG'GwC_Cy
Cuts at: 0 812 1182
Size: 812 370
PspOMI G'GGCC_C
Cuts at: 0 112 1182
Size: 112 1070
PstI C_TGCA'G
Cuts at: 0 1064 1182
Size: 1064 118
SacI G_AGCT'C
Cuts at: 0 232 1182
Size: 232 950
SfcI C'TryA_G
Cuts at: 0 509 1060 1182
Size: 509 551 122
Fragments arranged by size:
551 509 122
SmlI C'TyrA_G
Cuts at: 0 231 1182
Size: 231 951
StyI C'CwwG_G
Cuts at: 0 206 263 566 1182
Size: 206 57 303 616
Fragments arranged by size:
616 303 206 57
Tth111I GACn'n_nGTC
Cuts at: 0 309 1182
Size: 309 873
XbaI T'CTAG_A
Cuts at: 0 678 1182
Size: 678 504
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 171 1182
Size: 171 1011
XmnI GAAnn'nnTTC
Cuts at: 0 859 1027 1182
Size: 859 168 155
Fragments arranged by size:
859 168 155
Enzymes that do cut:
AccI AcuI ApaI ApoI BaeI BamHI BanII BbsI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BseRI
BseYI BsiHKAI Bsp1286I BspHI BstYI Bsu36I BtsI CspCI
DraIII DrdI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
Hin4I HindIII NdeI PflMI PpuMI PspOMI PstI SacI
SfcI SmlI StyI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvaI AvrII BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmtI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI
ClaI DraI EaeI EagI EarI EcoNI EcoRV FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI TstI XhoI XmaI ZraI