Restriction map for
pF1KB7649
(Linear) (Six-base) MAPSORT of: KIBB7649.seq Check: 2614 from: 1 to: 987
KIBB7649.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 602 695 987
Size: 583 19 93 292
Fragments arranged by size:
583 292 93 19
AleI CACnn'nnGTG
Cuts at: 0 736 987
Size: 736 251
ApaI G_GGCC'C
Cuts at: 0 31 987
Size: 31 956
ApaLI G'TGCA_C
Cuts at: 0 855 987
Size: 855 132
ApoI r'AATT_y
Cuts at: 0 312 987
Size: 312 675
AvaI C'yCGr_G
Cuts at: 0 30 92 932 987
Size: 30 62 840 55
Fragments arranged by size:
840 62 55 30
BamHI G'GATC_C
Cuts at: 0 687 987
Size: 687 300
BanI G'GyrC_C
Cuts at: 0 868 987
Size: 868 119
BanII G_rGCy'C
Cuts at: 0 31 626 987
Size: 31 595 361
Fragments arranged by size:
595 361 31
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 51 85 987
Size: 51 34 902
Fragments arranged by size:
902 51 34
BglI GCCn_nnn'nGGC
Cuts at: 0 56 987
Size: 56 931
BglII A'GATC_T
Cuts at: 0 247 987
Size: 247 740
BlpI GC'TnA_GC
Cuts at: 0 545 987
Size: 545 442
Bme1580I G_kGCm'C
Cuts at: 0 31 859 987
Size: 31 828 128
Fragments arranged by size:
828 128 31
BmgBI CAC'GTC
Cuts at: 0 641 987
Size: 641 346
BmrI ACTGGGnnnn_n'
Cuts at: 0 803 987
Size: 803 184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 381 508 688 987
Size: 7 374 127 180 299
Fragments arranged by size:
374 299 180 127 7
BsaI GGTCTCn'nnnn_
Cuts at: 0 272 987
Size: 272 715
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 260 987
Size: 260 727
BseYI C'CCAG_C
Cuts at: 0 8 438 987
Size: 8 430 549
Fragments arranged by size:
549 430 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 325 987
Size: 201 124 662
Fragments arranged by size:
662 201 124
BsiHKAI G_wGCw'C
Cuts at: 0 859 987
Size: 859 128
Bsp1286I G_dGCh'C
Cuts at: 0 31 626 859 987
Size: 31 595 233 128
Fragments arranged by size:
595 233 128 31
BssSI C'ACGA_G
Cuts at: 0 52 987
Size: 52 935
BstXI CCAn_nnnn'nTGG
Cuts at: 0 513 987
Size: 513 474
BstYI r'GATC_y
Cuts at: 0 247 687 987
Size: 247 440 300
Fragments arranged by size:
440 300 247
Bsu36I CC'TnA_GG
Cuts at: 0 702 953 987
Size: 702 251 34
Fragments arranged by size:
702 251 34
BtgI C'CryG_G
Cuts at: 0 37 987
Size: 37 950
EarI CTCTTCn'nnn_
Cuts at: 0 459 987
Size: 459 528
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 37 987
Size: 37 950
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 381 508 583 602 688 695 987
Size: 7 374 127 75 19 86 7 292
Fragments arranged by size:
374 292 127 86 75 19 7 7
EcoO109I rG'GnC_Cy
Cuts at: 0 27 336 987
Size: 27 309 651
Fragments arranged by size:
651 309 27
HaeII r_GCGC'y
Cuts at: 0 356 987
Size: 356 631
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 131 163 562 594 987
Size: 131 32 399 32 393
Fragments arranged by size:
399 393 131 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 494 987
Size: 494 493
MslI CAynn'nnrTG
Cuts at: 0 736 987
Size: 736 251
MspA1I CmG'CkG
Cuts at: 0 442 509 987
Size: 442 67 478
Fragments arranged by size:
478 442 67
NspI r_CATG'y
Cuts at: 0 931 987
Size: 931 56
PfoI T'CCnGG_A
Cuts at: 0 102 665 987
Size: 102 563 322
Fragments arranged by size:
563 322 102
PpuMI rG'GwC_Cy
Cuts at: 0 336 987
Size: 336 651
PspOMI G'GGCC_C
Cuts at: 0 27 987
Size: 27 960
PvuII CAG'CTG
Cuts at: 0 442 987
Size: 442 545
SfcI C'TryA_G
Cuts at: 0 239 987
Size: 239 748
SmaI CCC'GGG
Cuts at: 0 32 94 934 987
Size: 32 62 840 53
Fragments arranged by size:
840 62 53 32
SphI G_CATG'C
Cuts at: 0 931 987
Size: 931 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 22 987
Size: 22 965
TatI w'GTAC_w
Cuts at: 0 428 987
Size: 428 559
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 789 821 987
Size: 789 32 166
Fragments arranged by size:
789 166 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 645 987
Size: 645 342
XmaI C'CCGG_G
Cuts at: 0 30 92 932 987
Size: 30 62 840 55
Fragments arranged by size:
840 62 55 30
Enzymes that do cut:
AcuI AleI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BcgI BglI BglII BlpI Bme1580I BmgBI BmrI
BpmI BsaI BseRI BseYI BsgI BsiHKAI Bsp1286I BssSI
BstXI BstYI Bsu36I BtgI EarI EciI Eco57MI EcoO109I
HaeII Hin4I MmeI MslI MspA1I NspI PfoI PpuMI
PspOMI PvuII SfcI SmaI SphI TaqII TatI TstI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI AscI AseI AsiSI
AvrII BaeI BbeI BbsI BbvCI BciVI BclI BfrBI
BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI StuI StyI SwaI Tth111I XbaI XhoI
XmnI ZraI