Restriction map for
pF1KB7647
(Linear) (Six-base) MAPSORT of: KIBB7647.seq Check: 3382 from: 1 to: 984
KIBB7647.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
Acc65I G'GTAC_C
Cuts at: 0 389 984
Size: 389 595
AhdI GACnn_n'nnGTC
Cuts at: 0 36 205 984
Size: 36 169 779
Fragments arranged by size:
779 169 36
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 703 984
Size: 703 281
ApaI G_GGCC'C
Cuts at: 0 413 744 984
Size: 413 331 240
Fragments arranged by size:
413 331 240
AvaI C'yCGr_G
Cuts at: 0 326 984
Size: 326 658
BanI G'GyrC_C
Cuts at: 0 389 984
Size: 389 595
BanII G_rGCy'C
Cuts at: 0 235 333 413 744 984
Size: 235 98 80 331 240
Fragments arranged by size:
331 240 235 98 80
BbsI GAAGACnn'nnnn_
Cuts at: 0 824 984
Size: 824 160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 558 592 753 787 795 829 984
Size: 558 34 161 34 8 34 155
Fragments arranged by size:
558 161 155 34 34 34 8
BglI GCCn_nnn'nGGC
Cuts at: 0 68 984
Size: 68 916
BlpI GC'TnA_GC
Cuts at: 0 76 984
Size: 76 908
Bme1580I G_kGCm'C
Cuts at: 0 413 599 744 984
Size: 413 186 145 240
Fragments arranged by size:
413 240 186 145
BmgBI CAC'GTC
Cuts at: 0 57 984
Size: 57 927
BmrI ACTGGGnnnn_n'
Cuts at: 0 707 984
Size: 707 277
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 955 984
Size: 955 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 984
Size: 265 719
Bpu10I CC'TnA_GC
Cuts at: 0 565 984
Size: 565 419
BsaI GGTCTCn'nnnn_
Cuts at: 0 161 960 984
Size: 161 799 24
Fragments arranged by size:
799 161 24
BsaAI yAC'GTr
Cuts at: 0 876 984
Size: 876 108
BsaHI Gr'CG_yC
Cuts at: 0 162 984
Size: 162 822
BsaWI w'CCGG_w
Cuts at: 0 347 434 984
Size: 347 87 550
Fragments arranged by size:
550 347 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 342 372 886 916 984
Size: 342 30 514 30 68
Fragments arranged by size:
514 342 68 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 612 984
Size: 612 372
BseYI C'CCAG_C
Cuts at: 0 106 412 598 642 784 916 984
Size: 106 306 186 44 142 132 68
Fragments arranged by size:
306 186 142 132 106 68 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 984
Size: 443 541
Bsp1286I G_dGCh'C
Cuts at: 0 235 333 413 599 744 984
Size: 235 98 80 186 145 240
Fragments arranged by size:
240 235 186 145 98 80
BspEI T'CCGG_A
Cuts at: 0 347 434 984
Size: 347 87 550
Fragments arranged by size:
550 347 87
BsrBI CCG'CTC
Cuts at: 0 23 658 984
Size: 23 635 326
Fragments arranged by size:
635 326 23
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 301 984
Size: 57 244 683
Fragments arranged by size:
683 244 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 798 984
Size: 798 186
BtsI GCAGTG_nn'
Cuts at: 0 212 984
Size: 212 772
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 201 236 984
Size: 201 35 748
Fragments arranged by size:
748 201 35
EaeI y'GGCC_r
Cuts at: 0 86 984
Size: 86 898
EarI CTCTTCn'nnn_
Cuts at: 0 117 240 585 984
Size: 117 123 345 399
Fragments arranged by size:
399 345 123 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 984
Size: 265 719
EcoNI CCTnn'n_nnAGG
Cuts at: 0 376 984
Size: 376 608
EcoO109I rG'GnC_Cy
Cuts at: 0 399 409 740 984
Size: 399 10 331 244
Fragments arranged by size:
399 331 244 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 158 190 191 223 261 293
Size: 22 32 104 32 1 32 38 32
Cuts at: 293 984
Size: 691
Fragments arranged by size:
691 104 38 32 32 32 32 22
1
HincII GTy'rAC
Cuts at: 0 342 984
Size: 342 642
KpnI G_GTAC'C
Cuts at: 0 393 984
Size: 393 591
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 65 984
Size: 53 12 919
Fragments arranged by size:
919 53 12
MscI TGG'CCA
Cuts at: 0 88 984
Size: 88 896
MslI CAynn'nnrTG
Cuts at: 0 55 984
Size: 55 929
MspA1I CmG'CkG
Cuts at: 0 75 96 916 928 984
Size: 75 21 820 12 56
Fragments arranged by size:
820 75 56 21 12
PasI CC'CwG_GG
Cuts at: 0 907 984
Size: 907 77
PpuMI rG'GwC_Cy
Cuts at: 0 399 984
Size: 399 585
PspOMI G'GGCC_C
Cuts at: 0 409 740 984
Size: 409 331 244
Fragments arranged by size:
409 331 244
PstI C_TGCA'G
Cuts at: 0 830 984
Size: 830 154
PvuII CAG'CTG
Cuts at: 0 75 916 984
Size: 75 841 68
Fragments arranged by size:
841 75 68
SapI GCTCTTCn'nnn_
Cuts at: 0 117 240 984
Size: 117 123 744
Fragments arranged by size:
744 123 117
SfcI C'TryA_G
Cuts at: 0 826 857 984
Size: 826 31 127
Fragments arranged by size:
826 127 31
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 68 984
Size: 68 916
StyI C'CwwG_G
Cuts at: 0 11 984
Size: 11 973
Tth111I GACn'n_nGTC
Cuts at: 0 430 984
Size: 430 554
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 301 504 984
Size: 301 203 480
Fragments arranged by size:
480 301 203
Enzymes that do cut:
Acc65I AhdI AlfI ApaI AvaI BanI BanII BbsI
BcgI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI Bsp1286I BspEI BsrBI BstXI BtgZI BtsI CspCI
EaeI EarI Eco57MI EcoNI EcoO109I Hin4I HincII KpnI
MmeI MscI MslI MspA1I PasI PpuMI PspOMI PstI
PvuII SapI SfcI SfiI StyI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AleI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI
BfrBI BglII BmtI BpuEI BsaBI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspHI BspMI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
XbaI XhoI XmaI XmnI ZraI