Restriction map for
pF1KB7629
(Linear) (Six-base) MAPSORT of: KIBB7629.seq Check: 3488 from: 1 to: 957
KIBB7629.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 298 394 957
Size: 154 144 96 563
Fragments arranged by size:
563 154 144 96
AfeI AGC'GCT
Cuts at: 0 665 957
Size: 665 292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 682 957
Size: 682 275
AlwNI CAG_nnn'CTG
Cuts at: 0 334 957
Size: 334 623
ApoI r'AATT_y
Cuts at: 0 64 246 412 538 957
Size: 64 182 166 126 419
Fragments arranged by size:
419 182 166 126 64
AvaI C'yCGr_G
Cuts at: 0 641 957
Size: 641 316
BanII G_rGCy'C
Cuts at: 0 209 531 957
Size: 209 322 426
Fragments arranged by size:
426 322 209
BbsI GAAGACnn'nnnn_
Cuts at: 0 142 260 862 957
Size: 142 118 602 95
Fragments arranged by size:
602 142 118 95
BglI GCCn_nnn'nGGC
Cuts at: 0 724 957
Size: 724 233
BlpI GC'TnA_GC
Cuts at: 0 655 957
Size: 655 302
BmrI ACTGGGnnnn_n'
Cuts at: 0 582 957
Size: 582 375
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 545 957
Size: 406 139 412
Fragments arranged by size:
412 406 139
BsaHI Gr'CG_yC
Cuts at: 0 859 957
Size: 859 98
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 160 190 957
Size: 160 30 767
Fragments arranged by size:
767 160 30
BseYI C'CCAG_C
Cuts at: 0 850 957
Size: 850 107
BsiHKAI G_wGCw'C
Cuts at: 0 209 957
Size: 209 748
Bsp1286I G_dGCh'C
Cuts at: 0 209 531 957
Size: 209 322 426
Fragments arranged by size:
426 322 209
BsrDI GCAATG_nn'
Cuts at: 0 54 957
Size: 54 903
BstYI r'GATC_y
Cuts at: 0 129 828 867 957
Size: 129 699 39 90
Fragments arranged by size:
699 129 90 39
Bsu36I CC'TnA_GG
Cuts at: 0 74 957
Size: 74 883
BtgI C'CryG_G
Cuts at: 0 582 957
Size: 582 375
DraI TTT'AAA
Cuts at: 0 605 957
Size: 605 352
DraIII CAC_nnn'GTG
Cuts at: 0 156 957
Size: 156 801
EaeI y'GGCC_r
Cuts at: 0 687 957
Size: 687 270
EarI CTCTTCn'nnn_
Cuts at: 0 473 957
Size: 473 484
EcoICRI GAG'CTC
Cuts at: 0 207 957
Size: 207 750
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 298 394 406 545 957
Size: 154 144 96 12 139 412
Fragments arranged by size:
412 154 144 139 96 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 73 957
Size: 73 884
EcoRI G'AATT_C
Cuts at: 0 64 538 957
Size: 64 474 419
Fragments arranged by size:
474 419 64
HaeII r_GCGC'y
Cuts at: 0 641 667 957
Size: 641 26 290
Fragments arranged by size:
641 290 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 518 550 957
Size: 518 32 407
Fragments arranged by size:
518 407 32
HindIII A'AGCT_T
Cuts at: 0 238 957
Size: 238 719
MfeI C'AATT_G
Cuts at: 0 500 957
Size: 500 457
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 828 957
Size: 828 129
MscI TGG'CCA
Cuts at: 0 689 957
Size: 689 268
NspI r_CATG'y
Cuts at: 0 482 673 957
Size: 482 191 284
Fragments arranged by size:
482 284 191
PasI CC'CwG_GG
Cuts at: 0 162 957
Size: 162 795
PflMI CCAn_nnn'nTGG
Cuts at: 0 572 957
Size: 572 385
SacI G_AGCT'C
Cuts at: 0 209 957
Size: 209 748
SapI GCTCTTCn'nnn_
Cuts at: 0 473 957
Size: 473 484
SphI G_CATG'C
Cuts at: 0 482 957
Size: 482 475
SspI AAT'ATT
Cuts at: 0 905 957
Size: 905 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 160 192 957
Size: 160 32 765
Fragments arranged by size:
765 160 32
XmnI GAAnn'nnTTC
Cuts at: 0 68 957
Size: 68 889
Enzymes that do cut:
AcuI AfeI AlfI AlwNI ApoI AvaI BanII BbsI
BglI BlpI BmrI BpmI BsaHI BsaXI BseYI BsiHKAI
Bsp1286I BsrDI BstYI Bsu36I BtgI DraI DraIII EaeI
EarI EcoICRI Eco57MI EcoNI EcoRI HaeII Hin4I HindIII
MfeI MmeI MscI NspI PasI PflMI SacI SapI
SphI SspI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII Bme1580I BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BseRI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgZI BtsI ClaI CspCI DrdI EagI EciI EcoO109I
EcoRV FalI FseI FspI FspAI HincII HpaI KasI
KpnI MluI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI StuI StyI SwaI
TaqII TatI Tth111I XbaI XcmI XhoI XmaI ZraI