Restriction map for
pF1KB7628
(Linear) (Six-base) MAPSORT of: KIBB7628.seq Check: 2039 from: 1 to: 1119
KIBB7628.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 342 1119
Size: 342 777
Acc65I G'GTAC_C
Cuts at: 0 63 1119
Size: 63 1056
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 1119
Size: 518 601
AfeI AGC'GCT
Cuts at: 0 277 1119
Size: 277 842
AhdI GACnn_n'nnGTC
Cuts at: 0 547 1119
Size: 547 572
AvaI C'yCGr_G
Cuts at: 0 58 132 742 1011 1119
Size: 58 74 610 269 108
Fragments arranged by size:
610 269 108 74 58
BamHI G'GATC_C
Cuts at: 0 748 1119
Size: 748 371
BanI G'GyrC_C
Cuts at: 0 63 190 490 1119
Size: 63 127 300 629
Fragments arranged by size:
629 300 127 63
BanII G_rGCy'C
Cuts at: 0 856 1079 1119
Size: 856 223 40
Fragments arranged by size:
856 223 40
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 303 337 1119
Size: 303 34 782
Fragments arranged by size:
782 303 34
BfrBI ATG'CAT
Cuts at: 0 870 1119
Size: 870 249
BglI GCCn_nnn'nGGC
Cuts at: 0 773 1119
Size: 773 346
Bme1580I G_kGCm'C
Cuts at: 0 914 1119
Size: 914 205
BmrI ACTGGGnnnn_n'
Cuts at: 0 413 852 909 1119
Size: 413 439 57 210
Fragments arranged by size:
439 413 210 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1037 1119
Size: 1037 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 1119
Size: 247 872
BsaHI Gr'CG_yC
Cuts at: 0 178 1119
Size: 178 941
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1080 1110 1119
Size: 1080 30 9
Fragments arranged by size:
1080 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 170 397 1119
Size: 170 227 722
Fragments arranged by size:
722 227 170
BseYI C'CCAG_C
Cuts at: 0 979 1078 1119
Size: 979 99 41
Fragments arranged by size:
979 99 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 1119
Size: 504 615
BsiEI CG_ry'CG
Cuts at: 0 428 1119
Size: 428 691
BsiHKAI G_wGCw'C
Cuts at: 0 856 1042 1119
Size: 856 186 77
Fragments arranged by size:
856 186 77
BsmI GAATG_Cn'
Cuts at: 0 16 870 1119
Size: 16 854 249
Fragments arranged by size:
854 249 16
BsmBI CGTCTCn'nnnn_
Cuts at: 0 653 1119
Size: 653 466
Bsp1286I G_dGCh'C
Cuts at: 0 856 914 1042 1079 1119
Size: 856 58 128 37 40
Fragments arranged by size:
856 128 58 40 37
BspHI T'CATG_A
Cuts at: 0 473 1119
Size: 473 646
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 342 1119
Size: 342 777
BsrBI CCG'CTC
Cuts at: 0 261 1119
Size: 261 858
BsrDI GCAATG_nn'
Cuts at: 0 854 1119
Size: 854 265
BsrFI r'CCGG_y
Cuts at: 0 507 1119
Size: 507 612
BstAPI GCAn_nnn'nTGC
Cuts at: 0 311 1119
Size: 311 808
BstXI CCAn_nnnn'nTGG
Cuts at: 0 702 1119
Size: 702 417
BstYI r'GATC_y
Cuts at: 0 748 1119
Size: 748 371
BtgI C'CryG_G
Cuts at: 0 211 243 695 1119
Size: 211 32 452 424
Fragments arranged by size:
452 424 211 32
BtsI GCAGTG_nn'
Cuts at: 0 412 492 524 1055 1119
Size: 412 80 32 531 64
Fragments arranged by size:
531 412 80 64 32
EaeI y'GGCC_r
Cuts at: 0 122 344 425 509 1119
Size: 122 222 81 84 610
Fragments arranged by size:
610 222 122 84 81
EagI C'GGCC_G
Cuts at: 0 425 1119
Size: 425 694
EcoICRI GAG'CTC
Cuts at: 0 854 1119
Size: 854 265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 1119
Size: 518 601
EcoO109I rG'GnC_Cy
Cuts at: 0 774 1119
Size: 774 345
FspI TGC'GCA
Cuts at: 0 228 1119
Size: 228 891
FspAI rTGC'GCAy
Cuts at: 0 228 1119
Size: 228 891
HaeII r_GCGC'y
Cuts at: 0 112 140 279 1119
Size: 112 28 139 840
Fragments arranged by size:
840 139 112 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 533 565 925 957 1054 1086 1119
Size: 533 32 360 32 97 32 33
Fragments arranged by size:
533 360 97 33 32 32 32
KpnI G_GTAC'C
Cuts at: 0 67 1119
Size: 67 1052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 161 1052 1119
Size: 20 141 891 67
Fragments arranged by size:
891 141 67 20
MspA1I CmG'CkG
Cuts at: 0 128 213 1119
Size: 128 85 906
Fragments arranged by size:
906 128 85
NaeI GCC'GGC
Cuts at: 0 509 1119
Size: 509 610
NcoI C'CATG_G
Cuts at: 0 695 1119
Size: 695 424
NgoMIV G'CCGG_C
Cuts at: 0 507 1119
Size: 507 612
NsiI A_TGCA'T
Cuts at: 0 872 1119
Size: 872 247
NspI r_CATG'y
Cuts at: 0 288 589 1119
Size: 288 301 530
Fragments arranged by size:
530 301 288
PfoI T'CCnGG_A
Cuts at: 0 554 1119
Size: 554 565
PspXI vC'TCGA_Gb
Cuts at: 0 132 1119
Size: 132 987
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 232 264 1119
Size: 232 32 855
Fragments arranged by size:
855 232 32
PstI C_TGCA'G
Cuts at: 0 238 531 1119
Size: 238 293 588
Fragments arranged by size:
588 293 238
SacI G_AGCT'C
Cuts at: 0 856 1119
Size: 856 263
SacII CC_GC'GG
Cuts at: 0 214 1119
Size: 214 905
ScaI AGT'ACT
Cuts at: 0 691 1119
Size: 691 428
SexAI A'CCwGG_T
Cuts at: 0 605 1119
Size: 605 514
SfcI C'TryA_G
Cuts at: 0 234 527 627 1119
Size: 234 293 100 492
Fragments arranged by size:
492 293 234 100
SmaI CCC'GGG
Cuts at: 0 60 1119
Size: 60 1059
SmlI C'TyrA_G
Cuts at: 0 132 262 1011 1119
Size: 132 130 749 108
Fragments arranged by size:
749 132 130 108
StyI C'CwwG_G
Cuts at: 0 695 701 1119
Size: 695 6 418
Fragments arranged by size:
695 418 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 178 1119
Size: 178 941
TatI w'GTAC_w
Cuts at: 0 689 860 1119
Size: 689 171 259
Fragments arranged by size:
689 259 171
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 740 772 776 808 1080 1112 1119
Size: 740 32 4 32 272 32 7
Fragments arranged by size:
740 272 32 32 32 7 4
XhoI C'TCGA_G
Cuts at: 0 132 1011 1119
Size: 132 879 108
Fragments arranged by size:
879 132 108
XmaI C'CCGG_G
Cuts at: 0 58 1119
Size: 58 1061
Enzymes that do cut:
AarI Acc65I AcuI AfeI AhdI AvaI BamHI BanI
BanII BcgI BfrBI BglI Bme1580I BmrI BplI BpuEI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI
BstXI BstYI BtgI BtsI EaeI EagI EcoICRI Eco57MI
EcoO109I FspI FspAI HaeII Hin4I KpnI MmeI MspA1I
NaeI NcoI NgoMIV NsiI NspI PfoI PspXI PsrI
PstI SacI SacII ScaI SexAI SfcI SmaI SmlI
StyI TaqII TatI TstI XhoI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BbsI BbvCI BciVI BclI BglII
BlpI BmgBI BmtI BpmI Bpu10I BsaI BsaAI BsaBI
BsaWI BsiWI BspEI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EarI EciI EcoNI EcoRI EcoRV FalI FseI HincII
HindIII HpaI KasI MfeI MluI MscI MslI NarI
NdeI NheI NotI NruI PacI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PvuI
PvuII RsrII SalI SanDI SapI SbfI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XcmI XmnI ZraI