Restriction map for
pF1KB7623
(Linear) (Six-base) MAPSORT of: KIBB7623.seq Check: 3766 from: 1 to: 942
KIBB7623.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AccI GT'mk_AC
Cuts at: 0 818 942
Size: 818 124
AflIII A'CryG_T
Cuts at: 0 518 827 923 942
Size: 518 309 96 19
Fragments arranged by size:
518 309 96 19
AlwNI CAG_nnn'CTG
Cuts at: 0 456 942
Size: 456 486
ApaI G_GGCC'C
Cuts at: 0 86 200 691 907 942
Size: 86 114 491 216 35
Fragments arranged by size:
491 216 114 86 35
AvaI C'yCGr_G
Cuts at: 0 127 199 717 837 912 942
Size: 127 72 518 120 75 30
Fragments arranged by size:
518 127 120 75 72 30
BanI G'GyrC_C
Cuts at: 0 567 694 942
Size: 567 127 248
Fragments arranged by size:
567 248 127
BanII G_rGCy'C
Cuts at: 0 50 86 200 619 691 907 942
Size: 50 36 114 419 72 216 35
Fragments arranged by size:
419 216 114 72 50 36 35
BbeI G_GCGC'C
Cuts at: 0 571 698 942
Size: 571 127 244
Fragments arranged by size:
571 244 127
BglI GCCn_nnn'nGGC
Cuts at: 0 45 791 902 942
Size: 45 746 111 40
Fragments arranged by size:
746 111 45 40
Bme1580I G_kGCm'C
Cuts at: 0 86 200 691 907 942
Size: 86 114 491 216 35
Fragments arranged by size:
491 216 114 86 35
BmgBI CAC'GTC
Cuts at: 0 519 942
Size: 519 423
BmrI ACTGGGnnnn_n'
Cuts at: 0 558 942
Size: 558 384
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 942
Size: 49 893
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 942
Size: 844 98
BsaAI yAC'GTr
Cuts at: 0 480 830 942
Size: 480 350 112
Fragments arranged by size:
480 350 112
BsaHI Gr'CG_yC
Cuts at: 0 5 568 695 942
Size: 5 563 127 247
Fragments arranged by size:
563 247 127 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 373 651 741 942
Size: 373 278 90 201
Fragments arranged by size:
373 278 201 90
BseYI C'CCAG_C
Cuts at: 0 246 649 942
Size: 246 403 293
Fragments arranged by size:
403 293 246
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 942
Size: 523 419
BsiEI CG_ry'CG
Cuts at: 0 55 942
Size: 55 887
BsiWI C'GTAC_G
Cuts at: 0 277 788 942
Size: 277 511 154
Fragments arranged by size:
511 277 154
Bsp1286I G_dGCh'C
Cuts at: 0 50 86 200 619 691 907 942
Size: 50 36 114 419 72 216 35
Fragments arranged by size:
419 216 114 72 50 36 35
BsrBI CCG'CTC
Cuts at: 0 33 126 579 942
Size: 33 93 453 363
Fragments arranged by size:
453 363 93 33
BsrFI r'CCGG_y
Cuts at: 0 529 745 942
Size: 529 216 197
Fragments arranged by size:
529 216 197
BstAPI GCAn_nnn'nTGC
Cuts at: 0 227 942
Size: 227 715
BstXI CCAn_nnnn'nTGG
Cuts at: 0 564 942
Size: 564 378
DrdI GACnn_nn'nnGTC
Cuts at: 0 675 942
Size: 675 267
EaeI y'GGCC_r
Cuts at: 0 52 764 942
Size: 52 712 178
Fragments arranged by size:
712 178 52
EagI C'GGCC_G
Cuts at: 0 52 942
Size: 52 890
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 126 942
Size: 126 816
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 942
Size: 49 893
EcoNI CCTnn'n_nnAGG
Cuts at: 0 466 942
Size: 466 476
EcoO109I rG'GnC_Cy
Cuts at: 0 82 196 903 942
Size: 82 114 707 39
Fragments arranged by size:
707 114 82 39
HaeII r_GCGC'y
Cuts at: 0 571 577 698 782 942
Size: 571 6 121 84 160
Fragments arranged by size:
571 160 121 84 6
KasI G'GCGC_C
Cuts at: 0 567 694 942
Size: 567 127 248
Fragments arranged by size:
567 248 127
MluI A'CGCG_T
Cuts at: 0 923 942
Size: 923 19
MslI CAynn'nnrTG
Cuts at: 0 349 670 942
Size: 349 321 272
Fragments arranged by size:
349 321 272
MspA1I CmG'CkG
Cuts at: 0 267 456 494 621 942
Size: 267 189 38 127 321
Fragments arranged by size:
321 267 189 127 38
NaeI GCC'GGC
Cuts at: 0 531 942
Size: 531 411
NarI GG'CG_CC
Cuts at: 0 568 695 942
Size: 568 127 247
Fragments arranged by size:
568 247 127
NgoMIV G'CCGG_C
Cuts at: 0 529 942
Size: 529 413
NspI r_CATG'y
Cuts at: 0 711 942
Size: 711 231
PflMI CCAn_nnn'nTGG
Cuts at: 0 78 456 942
Size: 78 378 486
Fragments arranged by size:
486 378 78
PmlI CAC'GTG
Cuts at: 0 830 942
Size: 830 112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 304 336 942
Size: 304 32 606
Fragments arranged by size:
606 304 32
PspOMI G'GGCC_C
Cuts at: 0 82 196 687 903 942
Size: 82 114 491 216 39
Fragments arranged by size:
491 216 114 82 39
PspXI vC'TCGA_Gb
Cuts at: 0 837 942
Size: 837 105
PstI C_TGCA'G
Cuts at: 0 902 942
Size: 902 40
PvuII CAG'CTG
Cuts at: 0 456 942
Size: 456 486
SbfI CC_TGCA'GG
Cuts at: 0 902 942
Size: 902 40
SfcI C'TryA_G
Cuts at: 0 810 898 942
Size: 810 88 44
Fragments arranged by size:
810 88 44
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 902 942
Size: 902 40
SfoI GGC'GCC
Cuts at: 0 569 696 942
Size: 569 127 246
Fragments arranged by size:
569 246 127
SmlI C'TyrA_G
Cuts at: 0 127 837 859 942
Size: 127 710 22 83
Fragments arranged by size:
710 127 83 22
SphI G_CATG'C
Cuts at: 0 711 942
Size: 711 231
StyI C'CwwG_G
Cuts at: 0 191 942
Size: 191 751
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 663 942
Size: 663 279
TatI w'GTAC_w
Cuts at: 0 932 942
Size: 932 10
XhoI C'TCGA_G
Cuts at: 0 127 837 942
Size: 127 710 105
Fragments arranged by size:
710 127 105
Enzymes that do cut:
AccI AflIII AlwNI ApaI AvaI BanI BanII BbeI
BglI Bme1580I BmgBI BmrI BpmI BpuEI BsaAI BsaHI
BseRI BseYI BsgI BsiEI BsiWI Bsp1286I BsrBI BsrFI
BstAPI BstXI DrdI EaeI EagI EciI Eco57MI EcoNI
EcoO109I HaeII KasI MluI MslI MspA1I NaeI NarI
NgoMIV NspI PflMI PmlI PpiI PspOMI PspXI PstI
PvuII SbfI SfcI SfiI SfoI SmlI SphI StyI
TaqII TatI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmtI BplI Bpu10I BsaI
BsaBI BsaWI BsaXI BsiHKAI BsmI BsmBI BspEI BspHI
BspMI BsrDI BsrGI BssHII BssSI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII EarI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MmeI
MscI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PpuMI PshAI PsiI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI ScaI
SexAI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI