Restriction map for
pF1KB7615
(Linear) (Six-base) MAPSORT of: KIBB7615.seq Check: 609 from: 1 to: 912
KIBB7615.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 474 912
Size: 474 438
AhdI GACnn_n'nnGTC
Cuts at: 0 105 912
Size: 105 807
AleI CACnn'nnGTG
Cuts at: 0 881 912
Size: 881 31
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 770 912
Size: 770 142
AlwNI CAG_nnn'CTG
Cuts at: 0 261 323 459 912
Size: 261 62 136 453
Fragments arranged by size:
453 261 136 62
ApaI G_GGCC'C
Cuts at: 0 350 912
Size: 350 562
ApoI r'AATT_y
Cuts at: 0 613 912
Size: 613 299
AvaI C'yCGr_G
Cuts at: 0 115 761 912
Size: 115 646 151
Fragments arranged by size:
646 151 115
BanI G'GyrC_C
Cuts at: 0 200 275 912
Size: 200 75 637
Fragments arranged by size:
637 200 75
BanII G_rGCy'C
Cuts at: 0 122 350 406 912
Size: 122 228 56 506
Fragments arranged by size:
506 228 122 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 284 875 912
Size: 284 591 37
Fragments arranged by size:
591 284 37
BciVI GTATCCnnnnn_n'
Cuts at: 0 148 912
Size: 148 764
BglII A'GATC_T
Cuts at: 0 94 912
Size: 94 818
Bme1580I G_kGCm'C
Cuts at: 0 203 350 912
Size: 203 147 562
Fragments arranged by size:
562 203 147
BmrI ACTGGGnnnn_n'
Cuts at: 0 428 912
Size: 428 484
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 326 912
Size: 326 586
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 362 390 823 912
Size: 313 49 28 433 89
Fragments arranged by size:
433 313 89 49 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 49 79 912
Size: 49 30 833
Fragments arranged by size:
833 49 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 848 912
Size: 848 64
BsiEI CG_ry'CG
Cuts at: 0 450 912
Size: 450 462
BsiHKAI G_wGCw'C
Cuts at: 0 122 385 912
Size: 122 263 527
Fragments arranged by size:
527 263 122
Bsp1286I G_dGCh'C
Cuts at: 0 122 203 350 385 406 912
Size: 122 81 147 35 21 506
Fragments arranged by size:
506 147 122 81 35 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 474 912
Size: 474 438
BsrBI CCG'CTC
Cuts at: 0 12 912
Size: 12 900
BssHII G'CGCG_C
Cuts at: 0 431 912
Size: 431 481
BstAPI GCAn_nnn'nTGC
Cuts at: 0 54 616 912
Size: 54 562 296
Fragments arranged by size:
562 296 54
BstYI r'GATC_y
Cuts at: 0 94 672 912
Size: 94 578 240
Fragments arranged by size:
578 240 94
BtgI C'CryG_G
Cuts at: 0 41 87 912
Size: 41 46 825
Fragments arranged by size:
825 46 41
BtsI GCAGTG_nn'
Cuts at: 0 668 912
Size: 668 244
DraIII CAC_nnn'GTG
Cuts at: 0 304 912
Size: 304 608
EaeI y'GGCC_r
Cuts at: 0 447 912
Size: 447 465
EagI C'GGCC_G
Cuts at: 0 447 912
Size: 447 465
EarI CTCTTCn'nnn_
Cuts at: 0 161 396 721 912
Size: 161 235 325 191
Fragments arranged by size:
325 235 191 161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 490 729 912
Size: 490 239 183
Fragments arranged by size:
490 239 183
EcoICRI GAG'CTC
Cuts at: 0 120 912
Size: 120 792
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 362 390 823 912
Size: 313 49 28 433 89
Fragments arranged by size:
433 313 89 49 28
EcoO109I rG'GnC_Cy
Cuts at: 0 346 347 912
Size: 346 1 565
Fragments arranged by size:
565 346 1
FspI TGC'GCA
Cuts at: 0 28 905 912
Size: 28 877 7
Fragments arranged by size:
877 28 7
FspAI rTGC'GCAy
Cuts at: 0 905 912
Size: 905 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 91 123 877 909 912
Size: 91 32 754 32 3
Fragments arranged by size:
754 91 32 32 3
HincII GTy'rAC
Cuts at: 0 549 912
Size: 549 363
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 912
Size: 410 502
MslI CAynn'nnrTG
Cuts at: 0 881 912
Size: 881 31
NcoI C'CATG_G
Cuts at: 0 87 912
Size: 87 825
NdeI CA'TA_TG
Cuts at: 0 104 912
Size: 104 808
NspI r_CATG'y
Cuts at: 0 177 904 912
Size: 177 727 8
Fragments arranged by size:
727 177 8
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 28 912
Size: 28 884
PspOMI G'GGCC_C
Cuts at: 0 346 912
Size: 346 566
SacI G_AGCT'C
Cuts at: 0 122 912
Size: 122 790
SfcI C'TryA_G
Cuts at: 0 857 912
Size: 857 55
SphI G_CATG'C
Cuts at: 0 904 912
Size: 904 8
StyI C'CwwG_G
Cuts at: 0 87 912
Size: 87 825
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 49 81 912
Size: 49 32 831
Fragments arranged by size:
831 49 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 337 912
Size: 337 575
Enzymes that do cut:
AarI AhdI AleI AlfI AlwNI ApaI ApoI AvaI
BanI BanII BbsI BciVI BglII Bme1580I BmrI BplI
BpmI BsaXI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BssHII BstAPI BstYI BtgI BtsI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I FspI FspAI Hin4I
HincII MmeI MslI NcoI NdeI NspI PpiI PspOMI
SacI SfcI SphI StyI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BclI BfrBI BglI BlpI
BmgBI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrFI BsrGI BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EcoNI EcoRI
EcoRV FalI FseI HaeII HindIII HpaI KasI KpnI
MfeI MluI MscI MspA1I NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI StuI SwaI TaqII TatI
Tth111I XbaI XhoI XmaI XmnI ZraI