(Linear) (Six-base) MAPSORT of: KIBB7605.seq  Check: 2799  from: 1  to: 897



KIBB7605.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     897 
   Size:        185     712
 
AflII C'TTAA_G

Cuts at:      0     286     621     897 
   Size:        286     335     276

  Fragments arranged by size: 

                 335     286     276
 
ApoI r'AATT_y

Cuts at:      0     346     458     790     897 
   Size:        346     112     332     107

  Fragments arranged by size: 

                 346     332     112     107
 
AseI AT'TA_AT

Cuts at:      0      44     897 
   Size:         44     853
 
BanII G_rGCy'C

Cuts at:      0     218     897 
   Size:        218     679
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     511     676     897 
   Size:        511     165     221

  Fragments arranged by size: 

                 511     221     165
 
BclI T'GATC_A

Cuts at:      0     582     897 
   Size:        582     315
 
BglII A'GATC_T

Cuts at:      0     858     897 
   Size:        858      39
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     727     897 
   Size:        727     170
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      99     100     897 
   Size:         99       1     797

  Fragments arranged by size: 

                 797      99       1
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     724     897 
   Size:        724     173
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     745     897 
   Size:        745     152
 
BsiHKAI G_wGCw'C

Cuts at:      0      26     218     897 
   Size:         26     192     679

  Fragments arranged by size: 

                 679     192      26
 
Bsp1286I G_dGCh'C

Cuts at:      0      26     218     897 
   Size:         26     192     679

  Fragments arranged by size: 

                 679     192      26
 
BstYI r'GATC_y

Cuts at:      0     858     897 
   Size:        858      39
 
BtsI GCAGTG_nn'

Cuts at:      0     224     562     897 
   Size:        224     338     335

  Fragments arranged by size: 

                 338     335     224
 
EarI CTCTTCn'nnn_

Cuts at:      0     470     897 
   Size:        470     427
 
EcoICRI GAG'CTC

Cuts at:      0     216     897 
   Size:        216     681
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     727     897 
   Size:        185     542     170

  Fragments arranged by size: 

                 542     185     170
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     451     897 
   Size:        451     446
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      93     405     847     897 
   Size:         93     312     442      50

  Fragments arranged by size: 

                 442     312      93      50
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     145     177     193     225     352     384     529     561 
   Size:        145      32      16      32     127      32     145      32

Cuts at:    561     897 
   Size:        336

  Fragments arranged by size: 

                 336     145     145     127      32      32      32      32
                  16
 
MslI CAynn'nnrTG

Cuts at:      0      32     897 
   Size:         32     865
 
MspA1I CmG'CkG

Cuts at:      0     258     759     897 
   Size:        258     501     138

  Fragments arranged by size: 

                 501     258     138
 
PsiI TTA'TAA

Cuts at:      0     607     897 
   Size:        607     290
 
PvuII CAG'CTG

Cuts at:      0     258     759     897 
   Size:        258     501     138

  Fragments arranged by size: 

                 501     258     138
 
SacI G_AGCT'C

Cuts at:      0     218     897 
   Size:        218     679
 
SfcI C'TryA_G

Cuts at:      0      92     292     897 
   Size:         92     200     605

  Fragments arranged by size: 

                 605     200      92
 
SmlI C'TyrA_G

Cuts at:      0      78     115     286     621     897 
   Size:         78      37     171     335     276

  Fragments arranged by size: 

                 335     276     171      78      37
 

 Enzymes that do cut:

     AcuI    AflII     ApoI     AseI    BanII     BbsI     BclI    BglII
     BpmI    BpuEI    BseRI     BsgI  BsiHKAI Bsp1286I    BstYI     BtsI
     EarI  EcoICRI  Eco57MI    EcoNI     FalI    Hin4I     MslI   MspA1I
     PsiI    PvuII     SacI     SfcI     SmlI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI