Restriction map for
pF1KB7603
(Linear) (Six-base) MAPSORT of: KIBB7603.seq Check: 1999 from: 1 to: 891
KIBB7603.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
Acc65I G'GTAC_C
Cuts at: 0 206 691 891
Size: 206 485 200
Fragments arranged by size:
485 206 200
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 891
Size: 554 337
AflII C'TTAA_G
Cuts at: 0 13 891
Size: 13 878
ApaI G_GGCC'C
Cuts at: 0 101 891
Size: 101 790
ApoI r'AATT_y
Cuts at: 0 877 891
Size: 877 14
AvaI C'yCGr_G
Cuts at: 0 93 161 256 377 891
Size: 93 68 95 121 514
Fragments arranged by size:
514 121 95 93 68
BanI G'GyrC_C
Cuts at: 0 206 691 891
Size: 206 485 200
Fragments arranged by size:
485 206 200
BanII G_rGCy'C
Cuts at: 0 101 484 891
Size: 101 383 407
Fragments arranged by size:
407 383 101
BbsI GAAGACnn'nnnn_
Cuts at: 0 370 891
Size: 370 521
BfrBI ATG'CAT
Cuts at: 0 648 891
Size: 648 243
Bme1580I G_kGCm'C
Cuts at: 0 101 891
Size: 101 790
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 498 891
Size: 356 142 393
Fragments arranged by size:
393 356 142
BsaI GGTCTCn'nnnn_
Cuts at: 0 342 891
Size: 342 549
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 244 513 891
Size: 244 269 378
Fragments arranged by size:
378 269 244
BseYI C'CCAG_C
Cuts at: 0 483 726 891
Size: 483 243 165
Fragments arranged by size:
483 243 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 289 891
Size: 58 231 602
Fragments arranged by size:
602 231 58
BsiHKAI G_wGCw'C
Cuts at: 0 465 891
Size: 465 426
Bsp1286I G_dGCh'C
Cuts at: 0 101 465 484 891
Size: 101 364 19 407
Fragments arranged by size:
407 364 101 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 261 360 891
Size: 261 99 531
Fragments arranged by size:
531 261 99
BsrDI GCAATG_nn'
Cuts at: 0 446 891
Size: 446 445
BssSI C'ACGA_G
Cuts at: 0 870 891
Size: 870 21
BstAPI GCAn_nnn'nTGC
Cuts at: 0 193 891
Size: 193 698
BstXI CCAn_nnnn'nTGG
Cuts at: 0 751 891
Size: 751 140
BstYI r'GATC_y
Cuts at: 0 507 891
Size: 507 384
BtgI C'CryG_G
Cuts at: 0 83 686 891
Size: 83 603 205
Fragments arranged by size:
603 205 83
BtsI GCAGTG_nn'
Cuts at: 0 465 891
Size: 465 426
EaeI y'GGCC_r
Cuts at: 0 408 891
Size: 408 483
EarI CTCTTCn'nnn_
Cuts at: 0 285 531 891
Size: 285 246 360
Fragments arranged by size:
360 285 246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 498 554 891
Size: 356 142 56 337
Fragments arranged by size:
356 337 142 56
EcoO109I rG'GnC_Cy
Cuts at: 0 97 98 542 891
Size: 97 1 444 349
Fragments arranged by size:
444 349 97 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 846 891
Size: 846 45
HaeII r_GCGC'y
Cuts at: 0 141 891
Size: 141 750
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 144 176 499 531 546 563 578
Size: 26 118 32 323 32 15 17 15
Cuts at: 578 613 645 850 882 891
Size: 35 32 205 32 9
Fragments arranged by size:
323 205 118 35 32 32 32 32
26 17 15 15 9
HindIII A'AGCT_T
Cuts at: 0 762 823 891
Size: 762 61 68
Fragments arranged by size:
762 68 61
KpnI G_GTAC'C
Cuts at: 0 210 695 891
Size: 210 485 196
Fragments arranged by size:
485 210 196
MscI TGG'CCA
Cuts at: 0 410 891
Size: 410 481
MslI CAynn'nnrTG
Cuts at: 0 220 870 891
Size: 220 650 21
Fragments arranged by size:
650 220 21
NcoI C'CATG_G
Cuts at: 0 686 891
Size: 686 205
NsiI A_TGCA'T
Cuts at: 0 650 891
Size: 650 241
NspI r_CATG'y
Cuts at: 0 537 891
Size: 537 354
PasI CC'CwG_GG
Cuts at: 0 101 744 891
Size: 101 643 147
Fragments arranged by size:
643 147 101
PfoI T'CCnGG_A
Cuts at: 0 660 817 891
Size: 660 157 74
Fragments arranged by size:
660 157 74
PpuMI rG'GwC_Cy
Cuts at: 0 542 891
Size: 542 349
PsiI TTA'TAA
Cuts at: 0 800 854 891
Size: 800 54 37
Fragments arranged by size:
800 54 37
PspOMI G'GGCC_C
Cuts at: 0 97 891
Size: 97 794
SapI GCTCTTCn'nnn_
Cuts at: 0 285 891
Size: 285 606
SmaI CCC'GGG
Cuts at: 0 95 891
Size: 95 796
SmlI C'TyrA_G
Cuts at: 0 13 891
Size: 13 878
SphI G_CATG'C
Cuts at: 0 537 891
Size: 537 354
StyI C'CwwG_G
Cuts at: 0 686 891
Size: 686 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 757 891
Size: 757 134
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 666 698 891
Size: 666 32 193
Fragments arranged by size:
666 193 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 60 789 891
Size: 60 729 102
Fragments arranged by size:
729 102 60
XmaI C'CCGG_G
Cuts at: 0 93 891
Size: 93 798
Enzymes that do cut:
Acc65I AcuI AflII ApaI ApoI AvaI BanI BanII
BbsI BfrBI Bme1580I BpmI BsaI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrDI BssSI BstAPI BstXI BstYI
BtgI BtsI EaeI EarI Eco57MI EcoO109I FalI HaeII
Hin4I HindIII KpnI MscI MslI NcoI NsiI NspI
PasI PfoI PpuMI PsiI PspOMI SapI SmaI SmlI
SphI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HincII HpaI KasI MfeI MluI
MmeI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PciI PflMI PmeI PmlI PpiI PshAI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
TatI Tth111I XbaI XhoI XmnI ZraI