Restriction map for
pF1KB7591
(Linear) (Six-base) MAPSORT of: KIBB7591.seq Check: 3109 from: 1 to: 678
KIBB7591.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
Acc65I G'GTAC_C
Cuts at: 0 581 678
Size: 581 97
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 379 678
Size: 210 169 299
Fragments arranged by size:
299 210 169
AfeI AGC'GCT
Cuts at: 0 385 678
Size: 385 293
AleI CACnn'nnGTG
Cuts at: 0 103 678
Size: 103 575
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 79 678
Size: 79 599
AlwNI CAG_nnn'CTG
Cuts at: 0 402 604 678
Size: 402 202 74
Fragments arranged by size:
402 202 74
ApaI G_GGCC'C
Cuts at: 0 294 678
Size: 294 384
AvaI C'yCGr_G
Cuts at: 0 111 293 308 678
Size: 111 182 15 370
Fragments arranged by size:
370 182 111 15
BanI G'GyrC_C
Cuts at: 0 17 206 547 581 678
Size: 17 189 341 34 97
Fragments arranged by size:
341 189 97 34 17
BanII G_rGCy'C
Cuts at: 0 294 678
Size: 294 384
BbeI G_GCGC'C
Cuts at: 0 21 210 551 678
Size: 21 189 341 127
Fragments arranged by size:
341 189 127 21
BbvCI CC'TCA_GC
Cuts at: 0 649 678
Size: 649 29
BlpI GC'TnA_GC
Cuts at: 0 365 678
Size: 365 313
Bme1580I G_kGCm'C
Cuts at: 0 294 678
Size: 294 384
BmrI ACTGGGnnnn_n'
Cuts at: 0 95 226 678
Size: 95 131 452
Fragments arranged by size:
452 131 95
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 678
Size: 271 407
Bpu10I CC'TnA_GC
Cuts at: 0 649 678
Size: 649 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 678
Size: 676 2
BsaHI Gr'CG_yC
Cuts at: 0 18 207 548 678
Size: 18 189 341 130
Fragments arranged by size:
341 189 130 18
BseYI C'CCAG_C
Cuts at: 0 530 597 678
Size: 530 67 81
Fragments arranged by size:
530 81 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 256 678
Size: 164 92 422
Fragments arranged by size:
422 164 92
BsiHKAI G_wGCw'C
Cuts at: 0 120 678
Size: 120 558
Bsp1286I G_dGCh'C
Cuts at: 0 120 294 678
Size: 120 174 384
Fragments arranged by size:
384 174 120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 502 678
Size: 502 176
BsrFI r'CCGG_y
Cuts at: 0 607 678
Size: 607 71
BstXI CCAn_nnnn'nTGG
Cuts at: 0 105 678
Size: 105 573
BtgI C'CryG_G
Cuts at: 0 101 678
Size: 101 577
BtsI GCAGTG_nn'
Cuts at: 0 388 449 678
Size: 388 61 229
Fragments arranged by size:
388 229 61
EaeI y'GGCC_r
Cuts at: 0 80 609 678
Size: 80 529 69
Fragments arranged by size:
529 80 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 271 379 678
Size: 210 61 108 299
Fragments arranged by size:
299 210 108 61
EcoNI CCTnn'n_nnAGG
Cuts at: 0 129 143 264 325 678
Size: 129 14 121 61 353
Fragments arranged by size:
353 129 121 61 14
EcoO109I rG'GnC_Cy
Cuts at: 0 89 678
Size: 89 589
HaeII r_GCGC'y
Cuts at: 0 21 210 387 551 678
Size: 21 189 177 164 127
Fragments arranged by size:
189 177 164 127 21
KasI G'GCGC_C
Cuts at: 0 17 206 547 678
Size: 17 189 341 131
Fragments arranged by size:
341 189 131 17
KpnI G_GTAC'C
Cuts at: 0 585 678
Size: 585 93
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 221 678
Size: 221 457
MslI CAynn'nnrTG
Cuts at: 0 103 678
Size: 103 575
MspA1I CmG'CkG
Cuts at: 0 249 678
Size: 249 429
NaeI GCC'GGC
Cuts at: 0 609 678
Size: 609 69
NarI GG'CG_CC
Cuts at: 0 18 207 548 678
Size: 18 189 341 130
Fragments arranged by size:
341 189 130 18
NgoMIV G'CCGG_C
Cuts at: 0 607 678
Size: 607 71
PflMI CCAn_nnn'nTGG
Cuts at: 0 671 678
Size: 671 7
PfoI T'CCnGG_A
Cuts at: 0 670 678
Size: 670 8
PpuMI rG'GwC_Cy
Cuts at: 0 89 678
Size: 89 589
PspOMI G'GGCC_C
Cuts at: 0 290 678
Size: 290 388
PspXI vC'TCGA_Gb
Cuts at: 0 111 678
Size: 111 567
PstI C_TGCA'G
Cuts at: 0 130 678
Size: 130 548
PvuII CAG'CTG
Cuts at: 0 249 678
Size: 249 429
SanDI GG'GwC_CC
Cuts at: 0 89 678
Size: 89 589
SfcI C'TryA_G
Cuts at: 0 126 510 678
Size: 126 384 168
Fragments arranged by size:
384 168 126
SfoI GGC'GCC
Cuts at: 0 19 208 549 678
Size: 19 189 341 129
Fragments arranged by size:
341 189 129 19
SmaI CCC'GGG
Cuts at: 0 310 678
Size: 310 368
SmlI C'TyrA_G
Cuts at: 0 111 655 678
Size: 111 544 23
Fragments arranged by size:
544 111 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 611 678
Size: 611 67
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 668 678
Size: 668 10
XhoI C'TCGA_G
Cuts at: 0 111 678
Size: 111 567
XmaI C'CCGG_G
Cuts at: 0 308 678
Size: 308 370
Enzymes that do cut:
Acc65I AcuI AfeI AleI AlfI AlwNI ApaI AvaI
BanI BanII BbeI BbvCI BlpI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaHI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrFI BstXI BtgI BtsI EaeI Eco57MI EcoNI EcoO109I
HaeII KasI KpnI MmeI MslI MspA1I NaeI NarI
NgoMIV PflMI PfoI PpuMI PspOMI PspXI PstI PvuII
SanDI SfcI SfoI SmaI SmlI TaqII XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BcgI BciVI BclI BfrBI BglI BglII
BmgBI BmtI BplI BsaI BsaAI BsaBI BsaWI BsaXI
BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI MfeI MluI
MscI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PsrI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TatI TstI Tth111I
XbaI XmnI ZraI