Restriction map for
pF1KB7589
(Linear) (Six-base) MAPSORT of: KIBB7589.seq Check: 8425 from: 1 to: 672
KIBB7589.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AfeI AGC'GCT
Cuts at: 0 283 672
Size: 283 389
AflIII A'CryG_T
Cuts at: 0 323 672
Size: 323 349
AleI CACnn'nnGTG
Cuts at: 0 328 672
Size: 328 344
AlwNI CAG_nnn'CTG
Cuts at: 0 141 429 672
Size: 141 288 243
Fragments arranged by size:
288 243 141
ApaI G_GGCC'C
Cuts at: 0 602 672
Size: 602 70
ApaLI G'TGCA_C
Cuts at: 0 319 672
Size: 319 353
AvaI C'yCGr_G
Cuts at: 0 388 522 672
Size: 388 134 150
Fragments arranged by size:
388 150 134
BamHI G'GATC_C
Cuts at: 0 502 672
Size: 502 170
BanI G'GyrC_C
Cuts at: 0 3 15 160 609 672
Size: 3 12 145 449 63
Fragments arranged by size:
449 145 63 12 3
BanII G_rGCy'C
Cuts at: 0 374 465 602 672
Size: 374 91 137 70
Fragments arranged by size:
374 137 91 70
BbeI G_GCGC'C
Cuts at: 0 7 19 164 672
Size: 7 12 145 508
Fragments arranged by size:
508 145 12 7
BbvCI CC'TCA_GC
Cuts at: 0 593 672
Size: 593 79
BglI GCCn_nnn'nGGC
Cuts at: 0 568 672
Size: 568 104
Bme1580I G_kGCm'C
Cuts at: 0 323 602 614 672
Size: 323 279 12 58
Fragments arranged by size:
323 279 58 12
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 602 672
Size: 602 70
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 220 574 672
Size: 202 18 354 98
Fragments arranged by size:
354 202 98 18
Bpu10I CC'TnA_GC
Cuts at: 0 593 672
Size: 593 79
BsaAI yAC'GTr
Cuts at: 0 338 672
Size: 338 334
BsaHI Gr'CG_yC
Cuts at: 0 4 16 161 440 672
Size: 4 12 145 279 232
Fragments arranged by size:
279 232 145 12 4
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 529 559 672
Size: 529 30 113
Fragments arranged by size:
529 113 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 571 672
Size: 571 101
BseYI C'CCAG_C
Cuts at: 0 56 432 514 672
Size: 56 376 82 158
Fragments arranged by size:
376 158 82 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 672
Size: 190 482
BsiHKAI G_wGCw'C
Cuts at: 0 323 374 547 672
Size: 323 51 173 125
Fragments arranged by size:
323 173 125 51
BsmBI CGTCTCn'nnnn_
Cuts at: 0 55 672
Size: 55 617
Bsp1286I G_dGCh'C
Cuts at: 0 323 374 465 547 602 614 672
Size: 323 51 91 82 55 12 58
Fragments arranged by size:
323 91 82 58 55 51 12
BsrFI r'CCGG_y
Cuts at: 0 292 672
Size: 292 380
BssHII G'CGCG_C
Cuts at: 0 111 113 244 672
Size: 111 2 131 428
Fragments arranged by size:
428 131 111 2
BssSI C'ACGA_G
Cuts at: 0 313 672
Size: 313 359
BstAPI GCAn_nnn'nTGC
Cuts at: 0 141 318 672
Size: 141 177 354
Fragments arranged by size:
354 177 141
BstYI r'GATC_y
Cuts at: 0 426 502 672
Size: 426 76 170
Fragments arranged by size:
426 170 76
Bsu36I CC'TnA_GG
Cuts at: 0 566 672
Size: 566 106
BtgI C'CryG_G
Cuts at: 0 11 672
Size: 11 661
DraIII CAC_nnn'GTG
Cuts at: 0 318 672
Size: 318 354
DrdI GACnn_nn'nnGTC
Cuts at: 0 358 672
Size: 358 314
EaeI y'GGCC_r
Cuts at: 0 21 672
Size: 21 651
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 283 672
Size: 283 389
EcoICRI GAG'CTC
Cuts at: 0 372 672
Size: 372 300
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 220 574 672
Size: 202 18 354 98
Fragments arranged by size:
354 202 98 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 556 672
Size: 556 116
EcoO109I rG'GnC_Cy
Cuts at: 0 526 561 598 672
Size: 526 35 37 74
Fragments arranged by size:
526 74 37 35
HaeII r_GCGC'y
Cuts at: 0 7 19 164 285 497 672
Size: 7 12 145 121 212 175
Fragments arranged by size:
212 175 145 121 12 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 529 561 672
Size: 529 32 111
Fragments arranged by size:
529 111 32
KasI G'GCGC_C
Cuts at: 0 3 15 160 672
Size: 3 12 145 512
Fragments arranged by size:
512 145 12 3
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 672
Size: 572 100
MslI CAynn'nnrTG
Cuts at: 0 328 672
Size: 328 344
MspA1I CmG'CkG
Cuts at: 0 13 261 402 672
Size: 13 248 141 270
Fragments arranged by size:
270 248 141 13
NaeI GCC'GGC
Cuts at: 0 294 672
Size: 294 378
NarI GG'CG_CC
Cuts at: 0 4 16 161 672
Size: 4 12 145 511
Fragments arranged by size:
511 145 12 4
NgoMIV G'CCGG_C
Cuts at: 0 292 672
Size: 292 380
NspI r_CATG'y
Cuts at: 0 312 672
Size: 312 360
PasI CC'CwG_GG
Cuts at: 0 621 672
Size: 621 51
PfoI T'CCnGG_A
Cuts at: 0 422 498 672
Size: 422 76 174
Fragments arranged by size:
422 174 76
PmlI CAC'GTG
Cuts at: 0 338 672
Size: 338 334
PpuMI rG'GwC_Cy
Cuts at: 0 526 672
Size: 526 146
PspOMI G'GGCC_C
Cuts at: 0 598 672
Size: 598 74
PspXI vC'TCGA_Gb
Cuts at: 0 388 672
Size: 388 284
PstI C_TGCA'G
Cuts at: 0 137 266 672
Size: 137 129 406
Fragments arranged by size:
406 137 129
PvuII CAG'CTG
Cuts at: 0 261 672
Size: 261 411
SacI G_AGCT'C
Cuts at: 0 374 672
Size: 374 298
SacII CC_GC'GG
Cuts at: 0 14 672
Size: 14 658
SbfI CC_TGCA'GG
Cuts at: 0 137 672
Size: 137 535
SfcI C'TryA_G
Cuts at: 0 133 262 672
Size: 133 129 410
Fragments arranged by size:
410 133 129
SfoI GGC'GCC
Cuts at: 0 5 17 162 672
Size: 5 12 145 510
Fragments arranged by size:
510 145 12 5
SmaI CCC'GGG
Cuts at: 0 524 672
Size: 524 148
SmlI C'TyrA_G
Cuts at: 0 388 672
Size: 388 284
SphI G_CATG'C
Cuts at: 0 312 672
Size: 312 360
StuI AGG'CCT
Cuts at: 0 666 672
Size: 666 6
StyI C'CwwG_G
Cuts at: 0 667 672
Size: 667 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 526 558 672
Size: 526 32 114
Fragments arranged by size:
526 114 32
XhoI C'TCGA_G
Cuts at: 0 388 672
Size: 388 284
XmaI C'CCGG_G
Cuts at: 0 522 672
Size: 522 150
Enzymes that do cut:
AfeI AflIII AleI AlwNI ApaI ApaLI AvaI BamHI
BanI BanII BbeI BbvCI BglI Bme1580I BplI BpmI
Bpu10I BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BsrFI BssHII BssSI BstAPI BstYI Bsu36I
BtgI DraIII DrdI EaeI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI MmeI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PfoI PmlI PpuMI PspOMI
PspXI PstI PvuII SacI SacII SbfI SfcI SfoI
SmaI SmlI SphI StuI StyI TstI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BpuEI BsaI BsaBI BsaWI BsiEI
BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI
DraI EagI EarI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MscI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PflMI PmeI PpiI PshAI PsiI PsrI PvuI RsrII
SalI SanDI SapI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI TaqII TatI Tth111I
XbaI XcmI XmnI ZraI