Restriction map for
pF1KB7588
(Linear) (Six-base) MAPSORT of: KIBB7588.seq Check: 9979 from: 1 to: 867
KIBB7588.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 818 867
Size: 818 49
AatII G_ACGT'C
Cuts at: 0 70 867
Size: 70 797
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 867
Size: 104 763
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 550 582 754 786 867
Size: 550 32 172 32 81
Fragments arranged by size:
550 172 81 32 32
AvaI C'yCGr_G
Cuts at: 0 112 373 867
Size: 112 261 494
Fragments arranged by size:
494 261 112
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 768 801 867
Size: 768 33 66
Fragments arranged by size:
768 66 33
BanI G'GyrC_C
Cuts at: 0 316 867
Size: 316 551
BanII G_rGCy'C
Cuts at: 0 162 374 677 685 826 867
Size: 162 212 303 8 141 41
Fragments arranged by size:
303 212 162 141 41 8
BbeI G_GCGC'C
Cuts at: 0 320 867
Size: 320 547
BbsI GAAGACnn'nnnn_
Cuts at: 0 110 478 867
Size: 110 368 389
Fragments arranged by size:
389 368 110
BglI GCCn_nnn'nGGC
Cuts at: 0 508 867
Size: 508 359
BlpI GC'TnA_GC
Cuts at: 0 697 867
Size: 697 170
BmtI G_CTAG'C
Cuts at: 0 296 867
Size: 296 571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 609 644 778 867
Size: 609 35 134 89
Fragments arranged by size:
609 134 89 35
BsaHI Gr'CG_yC
Cuts at: 0 67 317 867
Size: 67 250 550
Fragments arranged by size:
550 250 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 280 310 867
Size: 280 30 557
Fragments arranged by size:
557 280 30
BseYI C'CCAG_C
Cuts at: 0 28 261 519 619 741 867
Size: 28 233 258 100 122 126
Fragments arranged by size:
258 233 126 122 100 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 867
Size: 806 61
BsiHKAI G_wGCw'C
Cuts at: 0 614 867
Size: 614 253
BsmBI CGTCTCn'nnnn_
Cuts at: 0 95 867
Size: 95 772
Bsp1286I G_dGCh'C
Cuts at: 0 162 374 614 677 685 826 867
Size: 162 212 240 63 8 141 41
Fragments arranged by size:
240 212 162 141 63 41 8
BspHI T'CATG_A
Cuts at: 0 584 867
Size: 584 283
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 818 867
Size: 818 49
BsrBI CCG'CTC
Cuts at: 0 692 867
Size: 692 175
BstXI CCAn_nnnn'nTGG
Cuts at: 0 668 756 867
Size: 668 88 111
Fragments arranged by size:
668 111 88
BstYI r'GATC_y
Cuts at: 0 565 867
Size: 565 302
DraIII CAC_nnn'GTG
Cuts at: 0 237 867
Size: 237 630
DrdI GACnn_nn'nnGTC
Cuts at: 0 22 867
Size: 22 845
EaeI y'GGCC_r
Cuts at: 0 446 509 867
Size: 446 63 358
Fragments arranged by size:
446 358 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 237 867
Size: 237 630
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 609 644 778 867
Size: 104 505 35 134 89
Fragments arranged by size:
505 134 104 89 35
EcoO109I rG'GnC_Cy
Cuts at: 0 24 867
Size: 24 843
HaeII r_GCGC'y
Cuts at: 0 320 341 847 867
Size: 320 21 506 20
Fragments arranged by size:
506 320 21 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 557 589 867
Size: 557 32 278
Fragments arranged by size:
557 278 32
HindIII A'AGCT_T
Cuts at: 0 269 867
Size: 269 598
KasI G'GCGC_C
Cuts at: 0 316 867
Size: 316 551
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 755 867
Size: 748 7 112
Fragments arranged by size:
748 112 7
MslI CAynn'nnrTG
Cuts at: 0 710 867
Size: 710 157
MspA1I CmG'CkG
Cuts at: 0 444 664 840 867
Size: 444 220 176 27
Fragments arranged by size:
444 220 176 27
NarI GG'CG_CC
Cuts at: 0 317 867
Size: 317 550
NheI G'CTAG_C
Cuts at: 0 292 867
Size: 292 575
PasI CC'CwG_GG
Cuts at: 0 678 867
Size: 678 189
PfoI T'CCnGG_A
Cuts at: 0 853 867
Size: 853 14
PpuMI rG'GwC_Cy
Cuts at: 0 24 867
Size: 24 843
PvuII CAG'CTG
Cuts at: 0 444 867
Size: 444 423
SanDI GG'GwC_CC
Cuts at: 0 24 867
Size: 24 843
SfcI C'TryA_G
Cuts at: 0 141 282 867
Size: 141 141 585
Fragments arranged by size:
585 141 141
SfoI GGC'GCC
Cuts at: 0 318 867
Size: 318 549
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 382 867
Size: 382 485
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 441 633 867
Size: 441 192 234
Fragments arranged by size:
441 234 192
ZraI GAC'GTC
Cuts at: 0 68 867
Size: 68 799
Enzymes that do cut:
AarI AatII AcuI AloI AvaI BaeI BanI BanII
BbeI BbsI BglI BlpI BmtI BpmI BsaHI BsaXI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BstXI BstYI DraIII DrdI EaeI EciI Eco57MI EcoO109I
HaeII Hin4I HindIII KasI MmeI MslI MspA1I NarI
NheI PasI PfoI PpuMI PvuII SanDI SfcI SfoI
TaqII XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BamHI BbvCI BcgI BciVI BclI BfrBI
BglII Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BseRI BsiEI BsiWI BsmI BspEI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
EagI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HpaI KpnI MfeI MluI MscI
NaeI NcoI NdeI NgoMIV NotI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TatI TstI Tth111I XbaI XhoI XmaI
XmnI