(Linear) (Six-base) MAPSORT of: KIBB7583.seq  Check: 1730  from: 1  to: 663



KIBB7583.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AccI GT'mk_AC

Cuts at:      0     270     663 
   Size:        270     393
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     406     663 
   Size:        349      57     257

  Fragments arranged by size: 

                 349     257      57
 
AfeI AGC'GCT

Cuts at:      0     460     663 
   Size:        460     203
 
AlwNI CAG_nnn'CTG

Cuts at:      0      33     663 
   Size:         33     630
 
BamHI G'GATC_C

Cuts at:      0     419     663 
   Size:        419     244
 
BanI G'GyrC_C

Cuts at:      0     246     663 
   Size:        246     417
 
BanII G_rGCy'C

Cuts at:      0     210     663 
   Size:        210     453
 
BmgBI CAC'GTC

Cuts at:      0     495     663 
   Size:        495     168
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     262     663 
   Size:        262     401
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58      76     247     418     552     663 
   Size:         58      18     171     171     134     111

  Fragments arranged by size: 

                 171     171     134     111      58      18
 
BsaBI GATnn'nnATC

Cuts at:      0      22     663 
   Size:         22     641
 
BsaWI w'CCGG_w

Cuts at:      0     422     663 
   Size:        422     241
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     441     663 
   Size:        441     222
 
BseYI C'CCAG_C

Cuts at:      0     405     663 
   Size:        405     258
 
BsiEI CG_ry'CG

Cuts at:      0      47     663 
   Size:         47     616
 
BsiHKAI G_wGCw'C

Cuts at:      0     505     663 
   Size:        505     158
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     453     663 
   Size:        453     210
 
Bsp1286I G_dGCh'C

Cuts at:      0     210     505     663 
   Size:        210     295     158

  Fragments arranged by size: 

                 295     210     158
 
BspEI T'CCGG_A

Cuts at:      0     422     663 
   Size:        422     241
 
BsrBI CCG'CTC

Cuts at:      0      60     564     663 
   Size:         60     504      99

  Fragments arranged by size: 

                 504      99      60
 
BstYI r'GATC_y

Cuts at:      0     419     663 
   Size:        419     244
 
BtsI GCAGTG_nn'

Cuts at:      0     549     594     663 
   Size:        549      45      69

  Fragments arranged by size: 

                 549      69      45
 
EaeI y'GGCC_r

Cuts at:      0      44     663 
   Size:         44     619
 
EagI C'GGCC_G

Cuts at:      0      44     663 
   Size:         44     619
 
EarI CTCTTCn'nnn_

Cuts at:      0     382     663 
   Size:        382     281
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58      76     247     349     406     418     552     663 
   Size:         58      18     171     102      57      12     134     111

  Fragments arranged by size: 

                 171     134     111     102      58      57      18      12
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     256     663 
   Size:        256     407
 
HaeII r_GCGC'y

Cuts at:      0     462     663 
   Size:        462     201
 
HincII GTy'rAC

Cuts at:      0     271     492     663 
   Size:        271     221     171

  Fragments arranged by size: 

                 271     221     171
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     488     494     663 
   Size:        488       6     169

  Fragments arranged by size: 

                 488     169       6
 
MslI CAynn'nnrTG

Cuts at:      0      28      86     663 
   Size:         28      58     577

  Fragments arranged by size: 

                 577      58      28
 
MspA1I CmG'CkG

Cuts at:      0     396     663 
   Size:        396     267
 
NspI r_CATG'y

Cuts at:      0     576     663 
   Size:        576      87
 
PpuMI rG'GwC_Cy

Cuts at:      0     256     663 
   Size:        256     407
 
PstI C_TGCA'G

Cuts at:      0     635     663 
   Size:        635      28
 
PvuII CAG'CTG

Cuts at:      0     396     663 
   Size:        396     267
 
SalI G'TCGA_C

Cuts at:      0     269     663 
   Size:        269     394
 
SanDI GG'GwC_CC

Cuts at:      0     256     663 
   Size:        256     407
 
SfcI C'TryA_G

Cuts at:      0     631     663 
   Size:        631      32
 
SphI G_CATG'C

Cuts at:      0     576     663 
   Size:        576      87
 

 Enzymes that do cut:

     AccI     AcuI     AfeI    AlwNI    BamHI     BanI    BanII    BmgBI
     BmrI     BpmI    BsaBI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BsrBI    BstYI     BtsI     EaeI     EagI
     EarI  Eco57MI EcoO109I    HaeII   HincII     MmeI     MslI   MspA1I
     NspI    PpuMI     PstI    PvuII     SalI    SanDI     SfcI     SphI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaXI     BsgI
    BsiWI     BsmI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI