(Linear) (Six-base) MAPSORT of: KIBB7575.seq  Check: 8721  from: 1  to: 648



KIBB7575.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AccI GT'mk_AC

Cuts at:      0     444     648 
   Size:        444     204
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     287     648 
   Size:        157     130     361

  Fragments arranged by size: 

                 361     157     130
 
ApoI r'AATT_y

Cuts at:      0     162     370     432     625     648 
   Size:        162     208      62     193      23

  Fragments arranged by size: 

                 208     193     162      62      23
 
BbvCI CC'TCA_GC

Cuts at:      0      94     648 
   Size:         94     554
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      71     105     648 
   Size:         71      34     543

  Fragments arranged by size: 

                 543      71      34
 
BmgBI CAC'GTC

Cuts at:      0     607     648 
   Size:        607      41
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     320     648 
   Size:        320     328
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47     648 
   Size:         47     601
 
Bpu10I CC'TnA_GC

Cuts at:      0      94     648 
   Size:         94     554
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     198     228     648 
   Size:        198      30     420

  Fragments arranged by size: 

                 420     198      30
 
BspHI T'CATG_A

Cuts at:      0     374     648 
   Size:        374     274
 
BsrDI GCAATG_nn'

Cuts at:      0      40     648 
   Size:         40     608
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      63     648 
   Size:         63     585
 
BstZ17I GTA'TAC

Cuts at:      0     445     648 
   Size:        445     203
 
BtsI GCAGTG_nn'

Cuts at:      0      52     648 
   Size:         52     596
 
DraI TTT'AAA

Cuts at:      0     644     648 
   Size:        644       4
 
EarI CTCTTCn'nnn_

Cuts at:      0     263     648 
   Size:        263     385
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47     157     287     648 
   Size:         47     110     130     361

  Fragments arranged by size: 

                 361     130     110      47
 
EcoRI G'AATT_C

Cuts at:      0     370     625     648 
   Size:        370     255      23

  Fragments arranged by size: 

                 370     255      23
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     388     420     648 
   Size:        388      32     228

  Fragments arranged by size: 

                 388     228      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     241     648 
   Size:        122     119     407

  Fragments arranged by size: 

                 407     122     119
 
MslI CAynn'nnrTG

Cuts at:      0     284     648 
   Size:        284     364
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     223     648 
   Size:        223     425
 
PsiI TTA'TAA

Cuts at:      0     205     648 
   Size:        205     443
 
SapI GCTCTTCn'nnn_

Cuts at:      0     263     648 
   Size:        263     385
 
StyI C'CwwG_G

Cuts at:      0      99     648 
   Size:         99     549
 
TatI w'GTAC_w

Cuts at:      0     580     648 
   Size:        580      68
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     183     215     648 
   Size:        183      32     433

  Fragments arranged by size: 

                 433     183      32
 

 Enzymes that do cut:

     AccI     AcuI     ApoI    BbvCI     BcgI    BmgBI     BmrI     BpmI
   Bpu10I    BsaXI    BspHI    BsrDI   BstAPI  BstZ17I     BtsI     DraI
     EarI  Eco57MI    EcoRI    Hin4I     MmeI     MslI    PflMI     PsiI
     SapI     StyI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI