Restriction map for
pF1KB7574
(Linear) (Six-base) MAPSORT of: KIBB7574.seq Check: 379 from: 1 to: 843
KIBB7574.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 764 843
Size: 764 79
AlwNI CAG_nnn'CTG
Cuts at: 0 501 843
Size: 501 342
ApaLI G'TGCA_C
Cuts at: 0 664 843
Size: 664 179
ApoI r'AATT_y
Cuts at: 0 220 843
Size: 220 623
AseI AT'TA_AT
Cuts at: 0 125 843
Size: 125 718
AvaI C'yCGr_G
Cuts at: 0 247 843
Size: 247 596
BanI G'GyrC_C
Cuts at: 0 74 843
Size: 74 769
BbsI GAAGACnn'nnnn_
Cuts at: 0 656 843
Size: 656 187
BbvCI CC'TCA_GC
Cuts at: 0 809 843
Size: 809 34
BglII A'GATC_T
Cuts at: 0 238 843
Size: 238 605
BlpI GC'TnA_GC
Cuts at: 0 494 843
Size: 494 349
Bme1580I G_kGCm'C
Cuts at: 0 668 843
Size: 668 175
BmrI ACTGGGnnnn_n'
Cuts at: 0 26 295 843
Size: 26 269 548
Fragments arranged by size:
548 269 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 182 843
Size: 182 661
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 843
Size: 596 247
Bpu10I CC'TnA_GC
Cuts at: 0 809 843
Size: 809 34
BsaI GGTCTCn'nnnn_
Cuts at: 0 74 174 843
Size: 74 100 669
Fragments arranged by size:
669 100 74
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 20 50 843
Size: 20 30 793
Fragments arranged by size:
793 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 70 843
Size: 28 42 773
Fragments arranged by size:
773 42 28
BsiEI CG_ry'CG
Cuts at: 0 16 843
Size: 16 827
BsiHKAI G_wGCw'C
Cuts at: 0 495 668 679 775 843
Size: 495 173 11 96 68
Fragments arranged by size:
495 173 96 68 11
BsmI GAATG_Cn'
Cuts at: 0 569 843
Size: 569 274
BsmBI CGTCTCn'nnnn_
Cuts at: 0 52 843
Size: 52 791
Bsp1286I G_dGCh'C
Cuts at: 0 495 668 679 775 843
Size: 495 173 11 96 68
Fragments arranged by size:
495 173 96 68 11
BsrDI GCAATG_nn'
Cuts at: 0 461 596 843
Size: 461 135 247
Fragments arranged by size:
461 247 135
BsrFI r'CCGG_y
Cuts at: 0 15 843
Size: 15 828
BsrGI T'GTAC_A
Cuts at: 0 141 843
Size: 141 702
BstXI CCAn_nnnn'nTGG
Cuts at: 0 93 843
Size: 93 750
BstYI r'GATC_y
Cuts at: 0 228 238 382 843
Size: 228 10 144 461
Fragments arranged by size:
461 228 144 10
Bsu36I CC'TnA_GG
Cuts at: 0 791 843
Size: 791 52
ClaI AT'CG_AT
Cuts at: 0 692 843
Size: 692 151
EaeI y'GGCC_r
Cuts at: 0 13 843
Size: 13 830
EagI C'GGCC_G
Cuts at: 0 13 843
Size: 13 830
EarI CTCTTCn'nnn_
Cuts at: 0 172 765 843
Size: 172 593 78
Fragments arranged by size:
593 172 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 764 843
Size: 596 168 79
Fragments arranged by size:
596 168 79
EcoO109I rG'GnC_Cy
Cuts at: 0 99 755 787 843
Size: 99 656 32 56
Fragments arranged by size:
656 99 56 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 71 103 762 794 843
Size: 71 32 659 32 49
Fragments arranged by size:
659 71 49 32 32
HincII GTy'rAC
Cuts at: 0 512 843
Size: 512 331
HpaI GTT'AAC
Cuts at: 0 512 843
Size: 512 331
MslI CAynn'nnrTG
Cuts at: 0 91 843
Size: 91 752
MspA1I CmG'CkG
Cuts at: 0 12 501 843
Size: 12 489 342
Fragments arranged by size:
489 342 12
NaeI GCC'GGC
Cuts at: 0 17 843
Size: 17 826
NdeI CA'TA_TG
Cuts at: 0 543 843
Size: 543 300
NgoMIV G'CCGG_C
Cuts at: 0 15 843
Size: 15 828
PacI TTA_AT'TAA
Cuts at: 0 482 843
Size: 482 361
PfoI T'CCnGG_A
Cuts at: 0 41 843
Size: 41 802
PpuMI rG'GwC_Cy
Cuts at: 0 755 843
Size: 755 88
PvuII CAG'CTG
Cuts at: 0 501 843
Size: 501 342
SfcI C'TryA_G
Cuts at: 0 427 843
Size: 427 416
SmaI CCC'GGG
Cuts at: 0 249 843
Size: 249 594
StuI AGG'CCT
Cuts at: 0 577 843
Size: 577 266
TatI w'GTAC_w
Cuts at: 0 141 843
Size: 141 702
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 39 843
Size: 39 804
XmaI C'CCGG_G
Cuts at: 0 247 843
Size: 247 596
Enzymes that do cut:
AcuI AlwNI ApaLI ApoI AseI AvaI BanI BbsI
BbvCI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BsaI BsaXI BseRI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BsrDI BsrFI BsrGI BstXI BstYI Bsu36I ClaI EaeI
EagI EarI Eco57MI EcoO109I Hin4I HincII HpaI MslI
MspA1I NaeI NdeI NgoMIV PacI PfoI PpuMI PvuII
SfcI SmaI StuI TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI AscI AsiSI
AvrII BaeI BamHI BanII BbeI BcgI BciVI BclI
BfrBI BglI BmgBI BmtI BpuEI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiWI BspEI BspHI BspMI BsrBI
BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII
KasI KpnI MfeI MluI MmeI MscI NarI NcoI
NheI NotI NruI NsiI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII
TstI Tth111I XbaI XhoI XmnI ZraI