Restriction map for
pF1KB7572
(Linear) (Six-base) MAPSORT of: KIBB7572.seq Check: 786 from: 1 to: 840
KIBB7572.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 111 840
Size: 111 729
AccI GT'mk_AC
Cuts at: 0 338 840
Size: 338 502
AclI AA'CG_TT
Cuts at: 0 494 840
Size: 494 346
AfeI AGC'GCT
Cuts at: 0 478 840
Size: 478 362
AlwNI CAG_nnn'CTG
Cuts at: 0 82 840
Size: 82 758
ApaI G_GGCC'C
Cuts at: 0 34 116 840
Size: 34 82 724
Fragments arranged by size:
724 82 34
ApaLI G'TGCA_C
Cuts at: 0 679 840
Size: 679 161
AvaI C'yCGr_G
Cuts at: 0 658 840
Size: 658 182
BamHI G'GATC_C
Cuts at: 0 712 840
Size: 712 128
BanI G'GyrC_C
Cuts at: 0 428 502 700 840
Size: 428 74 198 140
Fragments arranged by size:
428 198 140 74
BanII G_rGCy'C
Cuts at: 0 34 116 829 840
Size: 34 82 713 11
Fragments arranged by size:
713 82 34 11
BbeI G_GCGC'C
Cuts at: 0 506 704 840
Size: 506 198 136
Fragments arranged by size:
506 198 136
BbvCI CC'TCA_GC
Cuts at: 0 422 517 840
Size: 422 95 323
Fragments arranged by size:
422 323 95
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 725 759 840
Size: 725 34 81
Fragments arranged by size:
725 81 34
BclI T'GATC_A
Cuts at: 0 185 840
Size: 185 655
BglI GCCn_nnn'nGGC
Cuts at: 0 162 733 840
Size: 162 571 107
Fragments arranged by size:
571 162 107
BlpI GC'TnA_GC
Cuts at: 0 556 840
Size: 556 284
Bme1580I G_kGCm'C
Cuts at: 0 7 34 69 116 244 431 683 840
Size: 7 27 35 47 128 187 252 157
Fragments arranged by size:
252 187 157 128 47 35 27 7
Bpu10I CC'TnA_GC
Cuts at: 0 95 422 517 840
Size: 95 327 95 323
Fragments arranged by size:
327 323 95 95
BsaI GGTCTCn'nnnn_
Cuts at: 0 387 840
Size: 387 453
BsaHI Gr'CG_yC
Cuts at: 0 503 701 840
Size: 503 198 139
Fragments arranged by size:
503 198 139
BsaWI w'CCGG_w
Cuts at: 0 548 715 840
Size: 548 167 125
Fragments arranged by size:
548 167 125
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 141 142 158 840
Size: 141 1 16 682
Fragments arranged by size:
682 141 16 1
BseYI C'CCAG_C
Cuts at: 0 70 75 237 840
Size: 70 5 162 603
Fragments arranged by size:
603 162 70 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 840
Size: 289 551
BsiEI CG_ry'CG
Cuts at: 0 549 676 764 840
Size: 549 127 88 76
Fragments arranged by size:
549 127 88 76
BsiHKAI G_wGCw'C
Cuts at: 0 575 683 840
Size: 575 108 157
Fragments arranged by size:
575 157 108
Bsp1286I G_dGCh'C
Cuts at: 0 7 34 69 116 244 431 575 683
Size: 7 27 35 47 128 187 144 108
Cuts at: 683 829 840
Size: 146 11
Fragments arranged by size:
187 146 144 128 108 47 35 27
11 7
BspEI T'CCGG_A
Cuts at: 0 715 840
Size: 715 125
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 111 840
Size: 111 729
BsrBI CCG'CTC
Cuts at: 0 790 840
Size: 790 50
BsrFI r'CCGG_y
Cuts at: 0 380 675 840
Size: 380 295 165
Fragments arranged by size:
380 295 165
BssHII G'CGCG_C
Cuts at: 0 739 840
Size: 739 101
BstXI CCAn_nnnn'nTGG
Cuts at: 0 237 807 840
Size: 237 570 33
Fragments arranged by size:
570 237 33
BstYI r'GATC_y
Cuts at: 0 712 840
Size: 712 128
Bsu36I CC'TnA_GG
Cuts at: 0 11 840
Size: 11 829
DraIII CAC_nnn'GTG
Cuts at: 0 175 840
Size: 175 665
EaeI y'GGCC_r
Cuts at: 0 638 673 761 840
Size: 638 35 88 79
Fragments arranged by size:
638 88 79 35
EagI C'GGCC_G
Cuts at: 0 673 761 840
Size: 673 88 79
Fragments arranged by size:
673 88 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 565 840
Size: 565 275
EcoNI CCTnn'n_nnAGG
Cuts at: 0 421 840
Size: 421 419
EcoO109I rG'GnC_Cy
Cuts at: 0 31 113 206 355 646 687 840
Size: 31 82 93 149 291 41 153
Fragments arranged by size:
291 153 149 93 82 41 31
HaeII r_GCGC'y
Cuts at: 0 82 480 506 704 840
Size: 82 398 26 198 136
Fragments arranged by size:
398 198 136 82 26
HincII GTy'rAC
Cuts at: 0 498 840
Size: 498 342
HpaI GTT'AAC
Cuts at: 0 498 840
Size: 498 342
KasI G'GCGC_C
Cuts at: 0 502 700 840
Size: 502 198 140
Fragments arranged by size:
502 198 140
MslI CAynn'nnrTG
Cuts at: 0 805 840
Size: 805 35
MspA1I CmG'CkG
Cuts at: 0 258 840
Size: 258 582
NarI GG'CG_CC
Cuts at: 0 503 701 840
Size: 503 198 139
Fragments arranged by size:
503 198 139
PasI CC'CwG_GG
Cuts at: 0 211 276 840
Size: 211 65 564
Fragments arranged by size:
564 211 65
PflMI CCAn_nnn'nTGG
Cuts at: 0 444 840
Size: 444 396
PpuMI rG'GwC_Cy
Cuts at: 0 355 687 840
Size: 355 332 153
Fragments arranged by size:
355 332 153
PspOMI G'GGCC_C
Cuts at: 0 30 112 840
Size: 30 82 728
Fragments arranged by size:
728 82 30
SanDI GG'GwC_CC
Cuts at: 0 687 840
Size: 687 153
SexAI A'CCwGG_T
Cuts at: 0 171 840
Size: 171 669
SfoI GGC'GCC
Cuts at: 0 504 702 840
Size: 504 198 138
Fragments arranged by size:
504 198 138
SmaI CCC'GGG
Cuts at: 0 660 840
Size: 660 180
TatI w'GTAC_w
Cuts at: 0 590 840
Size: 590 250
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 234 840
Size: 234 606
XmaI C'CCGG_G
Cuts at: 0 658 840
Size: 658 182
Enzymes that do cut:
AarI AccI AclI AfeI AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BclI BglI
BlpI Bme1580I Bpu10I BsaI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI
BssHII BstXI BstYI Bsu36I DraIII EaeI EagI EciI
EcoNI EcoO109I HaeII HincII HpaI KasI MslI MspA1I
NarI PasI PflMI PpuMI PspOMI SanDI SexAI SfoI
SmaI TatI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AcuI AflII AflIII AgeI AhdI AleI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BbsI BciVI BfrBI BglII BmgBI BmrI BmtI BplI
BpmI BpuEI BsaAI BsaBI BsaXI BsiWI BsmI BsmBI
BspHI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EarI
EcoICRI Eco57MI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HindIII KpnI MfeI MluI MmeI MscI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TstI Tth111I XbaI XhoI XmnI ZraI