Restriction map for
pF1KB7362
(Linear) (Six-base) MAPSORT of: KIBB7362.seq Check: 438 from: 1 to: 756
KIBB7362.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AflII C'TTAA_G
Cuts at: 0 543 756
Size: 543 213
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 70 102 756
Size: 70 32 654
Fragments arranged by size:
654 70 32
ApaI G_GGCC'C
Cuts at: 0 107 138 756
Size: 107 31 618
Fragments arranged by size:
618 107 31
AvaI C'yCGr_G
Cuts at: 0 58 91 756
Size: 58 33 665
Fragments arranged by size:
665 58 33
BanI G'GyrC_C
Cuts at: 0 97 499 756
Size: 97 402 257
Fragments arranged by size:
402 257 97
BanII G_rGCy'C
Cuts at: 0 107 138 176 756
Size: 107 31 38 580
Fragments arranged by size:
580 107 38 31
BbeI G_GCGC'C
Cuts at: 0 101 756
Size: 101 655
BbsI GAAGACnn'nnnn_
Cuts at: 0 641 733 756
Size: 641 92 23
Fragments arranged by size:
641 92 23
BfrBI ATG'CAT
Cuts at: 0 743 756
Size: 743 13
BglII A'GATC_T
Cuts at: 0 237 756
Size: 237 519
Bme1580I G_kGCm'C
Cuts at: 0 107 138 535 756
Size: 107 31 397 221
Fragments arranged by size:
397 221 107 31
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 684 756
Size: 684 72
Bpu10I CC'TnA_GC
Cuts at: 0 267 756
Size: 267 489
BsaAI yAC'GTr
Cuts at: 0 111 756
Size: 111 645
BsaHI Gr'CG_yC
Cuts at: 0 98 756
Size: 98 658
BseYI C'CCAG_C
Cuts at: 0 579 756
Size: 579 177
BsiHKAI G_wGCw'C
Cuts at: 0 335 436 602 756
Size: 335 101 166 154
Fragments arranged by size:
335 166 154 101
Bsp1286I G_dGCh'C
Cuts at: 0 107 138 176 335 436 535 602 756
Size: 107 31 38 159 101 99 67 154
Fragments arranged by size:
159 154 107 101 99 67 38 31
BsrBI CCG'CTC
Cuts at: 0 360 756
Size: 360 396
BsrFI r'CCGG_y
Cuts at: 0 69 756
Size: 69 687
BssHII G'CGCG_C
Cuts at: 0 6 8 756
Size: 6 2 748
Fragments arranged by size:
748 6 2
BstXI CCAn_nnnn'nTGG
Cuts at: 0 579 756
Size: 579 177
BstYI r'GATC_y
Cuts at: 0 237 756
Size: 237 519
BtgI C'CryG_G
Cuts at: 0 51 299 342 441 756
Size: 51 248 43 99 315
Fragments arranged by size:
315 248 99 51 43
BtsI GCAGTG_nn'
Cuts at: 0 310 756
Size: 310 446
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 556 591 756
Size: 556 35 165
Fragments arranged by size:
556 165 35
EarI CTCTTCn'nnn_
Cuts at: 0 371 756
Size: 371 385
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 469 756
Size: 469 287
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 684 756
Size: 684 72
EcoNI CCTnn'n_nnAGG
Cuts at: 0 121 756
Size: 121 635
EcoO109I rG'GnC_Cy
Cuts at: 0 104 756
Size: 104 652
HaeII r_GCGC'y
Cuts at: 0 51 101 463 756
Size: 51 50 362 293
Fragments arranged by size:
362 293 51 50
KasI G'GCGC_C
Cuts at: 0 97 756
Size: 97 659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 353 756
Size: 308 45 403
Fragments arranged by size:
403 308 45
MslI CAynn'nnrTG
Cuts at: 0 738 748 756
Size: 738 10 8
Fragments arranged by size:
738 10 8
MspA1I CmG'CkG
Cuts at: 0 53 492 756
Size: 53 439 264
Fragments arranged by size:
439 264 53
NaeI GCC'GGC
Cuts at: 0 71 756
Size: 71 685
NarI GG'CG_CC
Cuts at: 0 98 756
Size: 98 658
NcoI C'CATG_G
Cuts at: 0 299 441 756
Size: 299 142 315
Fragments arranged by size:
315 299 142
NgoMIV G'CCGG_C
Cuts at: 0 69 756
Size: 69 687
NsiI A_TGCA'T
Cuts at: 0 745 756
Size: 745 11
PspOMI G'GGCC_C
Cuts at: 0 103 134 756
Size: 103 31 622
Fragments arranged by size:
622 103 31
PstI C_TGCA'G
Cuts at: 0 317 756
Size: 317 439
SacII CC_GC'GG
Cuts at: 0 54 756
Size: 54 702
SfcI C'TryA_G
Cuts at: 0 313 756
Size: 313 443
SfoI GGC'GCC
Cuts at: 0 99 756
Size: 99 657
SmlI C'TyrA_G
Cuts at: 0 543 756
Size: 543 213
StyI C'CwwG_G
Cuts at: 0 299 441 756
Size: 299 142 315
Fragments arranged by size:
315 299 142
XbaI T'CTAG_A
Cuts at: 0 591 756
Size: 591 165
Enzymes that do cut:
AflII AloI ApaI AvaI BanI BanII BbeI BbsI
BfrBI BglII Bme1580I BpmI Bpu10I BsaAI BsaHI BseYI
BsiHKAI Bsp1286I BsrBI BsrFI BssHII BstXI BstYI BtgI
BtsI CspCI EarI EciI Eco57MI EcoNI EcoO109I HaeII
KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI PspOMI PstI SacII SfcI SfoI SmlI StyI
XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BglI BlpI BmgBI BmrI BmtI BplI BpuEI
BsaI BsaBI BsaWI BsaXI BseRI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI
DraIII DrdI EaeI EagI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MscI NdeI NheI NotI NruI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XcmI XhoI
XmaI XmnI ZraI