Restriction map for
pF1KB7348
(Linear) (Six-base) MAPSORT of: KIBB7348.seq Check: 4171 from: 1 to: 3357
KIBB7348.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2925 3357
Size: 2925 432
AccI GT'mk_AC
Cuts at: 0 681 1103 1263 2617 3357
Size: 681 422 160 1354 740
Fragments arranged by size:
1354 740 681 422 160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1040 2676 2709 3172 3357
Size: 1040 1636 33 463 185
Fragments arranged by size:
1636 1040 463 185 33
AflII C'TTAA_G
Cuts at: 0 106 1825 3357
Size: 106 1719 1532
Fragments arranged by size:
1719 1532 106
AleI CACnn'nnGTG
Cuts at: 0 502 3010 3357
Size: 502 2508 347
Fragments arranged by size:
2508 502 347
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 224 3357
Size: 224 3133
ApaI G_GGCC'C
Cuts at: 0 1276 3357
Size: 1276 2081
ApaLI G'TGCA_C
Cuts at: 0 516 785 816 1617 3357
Size: 516 269 31 801 1740
Fragments arranged by size:
1740 801 516 269 31
ApoI r'AATT_y
Cuts at: 0 1074 1183 2002 2163 2295 2372 2532 2570
Size: 1074 109 819 161 132 77 160 38
Cuts at: 2570 2676 2859 3357
Size: 106 183 498
Fragments arranged by size:
1074 819 498 183 161 160 132 109
106 77 38
AseI AT'TA_AT
Cuts at: 0 1004 3357
Size: 1004 2353
BamHI G'GATC_C
Cuts at: 0 2685 3357
Size: 2685 672
BanI G'GyrC_C
Cuts at: 0 26 1093 3357
Size: 26 1067 2264
Fragments arranged by size:
2264 1067 26
BanII G_rGCy'C
Cuts at: 0 50 1276 2154 3191 3357
Size: 50 1226 878 1037 166
Fragments arranged by size:
1226 1037 878 166 50
BbeI G_GCGC'C
Cuts at: 0 30 3357
Size: 30 3327
BbsI GAAGACnn'nnnn_
Cuts at: 0 1232 1643 1946 3357
Size: 1232 411 303 1411
Fragments arranged by size:
1411 1232 411 303
BbvCI CC'TCA_GC
Cuts at: 0 2894 3357
Size: 2894 463
BciVI GTATCCnnnnn_n'
Cuts at: 0 3026 3357
Size: 3026 331
BclI T'GATC_A
Cuts at: 0 506 761 957 2268 3200 3357
Size: 506 255 196 1311 932 157
Fragments arranged by size:
1311 932 506 255 196 157
BfrBI ATG'CAT
Cuts at: 0 638 1258 1898 2911 3357
Size: 638 620 640 1013 446
Fragments arranged by size:
1013 640 638 620 446
BglII A'GATC_T
Cuts at: 0 3031 3357
Size: 3031 326
BlpI GC'TnA_GC
Cuts at: 0 566 3357
Size: 566 2791
Bme1580I G_kGCm'C
Cuts at: 0 520 789 820 1098 1276 1621 3357
Size: 520 269 31 278 178 345 1736
Fragments arranged by size:
1736 520 345 278 269 178 31
BmrI ACTGGGnnnn_n'
Cuts at: 0 793 3086 3357
Size: 793 2293 271
Fragments arranged by size:
2293 793 271
BmtI G_CTAG'C
Cuts at: 0 973 3357
Size: 973 2384
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3240 3357
Size: 3240 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 1832 3357
Size: 53 1779 1525
Fragments arranged by size:
1779 1525 53
Bpu10I CC'TnA_GC
Cuts at: 0 2894 3357
Size: 2894 463
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 3357
Size: 472 2885
BsaI GGTCTCn'nnnn_
Cuts at: 0 922 2191 3357
Size: 922 1269 1166
Fragments arranged by size:
1269 1166 922
BsaBI GATnn'nnATC
Cuts at: 0 3217 3357
Size: 3217 140
BsaHI Gr'CG_yC
Cuts at: 0 27 3357
Size: 27 3330
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 374 389 1235 1442 3357
Size: 374 15 846 207 1915
Fragments arranged by size:
1915 846 374 207 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 1634 3357
Size: 436 1198 1723
Fragments arranged by size:
1723 1198 436
BsiHKAI G_wGCw'C
Cuts at: 0 520 789 820 1621 2154 3191 3357
Size: 520 269 31 801 533 1037 166
Fragments arranged by size:
1037 801 533 520 269 166 31
BsmI GAATG_Cn'
Cuts at: 0 640 1900 1929 3357
Size: 640 1260 29 1428
Fragments arranged by size:
1428 1260 640 29
Bsp1286I G_dGCh'C
Cuts at: 0 50 520 789 820 1098 1276 1621 2154
Size: 50 470 269 31 278 178 345 533
Cuts at: 2154 3191 3357
Size: 1037 166
Fragments arranged by size:
1037 533 470 345 278 269 178 166
50 31
BspHI T'CATG_A
Cuts at: 0 924 2123 2855 3357
Size: 924 1199 732 502
Fragments arranged by size:
1199 924 732 502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2925 3357
Size: 2925 432
BsrDI GCAATG_nn'
Cuts at: 0 509 538 2069 2418 2503 2701 2888 3357
Size: 509 29 1531 349 85 198 187 469
Fragments arranged by size:
1531 509 469 349 198 187 85 29
BsrFI r'CCGG_y
Cuts at: 0 3146 3357
Size: 3146 211
BsrGI T'GTAC_A
Cuts at: 0 1146 3357
Size: 1146 2211
BstAPI GCAn_nnn'nTGC
Cuts at: 0 549 3357
Size: 549 2808
BstEII G'GTnAC_C
Cuts at: 0 1432 3357
Size: 1432 1925
BstYI r'GATC_y
Cuts at: 0 2170 2685 3031 3156 3357
Size: 2170 515 346 125 201
Fragments arranged by size:
2170 515 346 201 125
Bsu36I CC'TnA_GG
Cuts at: 0 13 3357
Size: 13 3344
BtgI C'CryG_G
Cuts at: 0 1565 3357
Size: 1565 1792
BtsI GCAGTG_nn'
Cuts at: 0 1646 2497 3357
Size: 1646 851 860
Fragments arranged by size:
1646 860 851
ClaI AT'CG_AT
Cuts at: 0 1013 3357
Size: 1013 2344
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 840 875 3357
Size: 840 35 2482
Fragments arranged by size:
2482 840 35
EarI CTCTTCn'nnn_
Cuts at: 0 659 2914 3357
Size: 659 2255 443
Fragments arranged by size:
2255 659 443
EcoICRI GAG'CTC
Cuts at: 0 2152 3189 3357
Size: 2152 1037 168
Fragments arranged by size:
2152 1037 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 1040 1832 2676 2709 3172 3357
Size: 53 987 792 844 33 463 185
Fragments arranged by size:
987 844 792 463 185 53 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1386 3357
Size: 1386 1971
EcoO109I rG'GnC_Cy
Cuts at: 0 1272 1273 3357
Size: 1272 1 2084
Fragments arranged by size:
2084 1272 1
EcoRI G'AATT_C
Cuts at: 0 2002 2295 3357
Size: 2002 293 1062
Fragments arranged by size:
2002 1062 293
EcoRV GAT'ATC
Cuts at: 0 875 2745 3357
Size: 875 1870 612
Fragments arranged by size:
1870 875 612
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1453 3357
Size: 1453 1904
HaeII r_GCGC'y
Cuts at: 0 30 3357
Size: 30 3327
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 384 416 1324 1356 1821 1853 2986 3018
Size: 384 32 908 32 465 32 1133 32
Cuts at: 3018 3357
Size: 339
Fragments arranged by size:
1133 908 465 384 339 32 32 32
32
HincII GTy'rAC
Cuts at: 0 906 3357
Size: 906 2451
HpaI GTT'AAC
Cuts at: 0 906 3357
Size: 906 2451
KasI G'GCGC_C
Cuts at: 0 26 3357
Size: 26 3331
MfeI C'AATT_G
Cuts at: 0 2507 2846 3357
Size: 2507 339 511
Fragments arranged by size:
2507 511 339
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2403 2567 3357
Size: 2403 164 790
Fragments arranged by size:
2403 790 164
MslI CAynn'nnrTG
Cuts at: 0 412 502 2194 2452 3010 3357
Size: 412 90 1692 258 558 347
Fragments arranged by size:
1692 558 412 347 258 90
MspA1I CmG'CkG
Cuts at: 0 502 3357
Size: 502 2855
NarI GG'CG_CC
Cuts at: 0 27 3357
Size: 27 3330
NcoI C'CATG_G
Cuts at: 0 1565 3357
Size: 1565 1792
NdeI CA'TA_TG
Cuts at: 0 132 2156 3357
Size: 132 2024 1201
Fragments arranged by size:
2024 1201 132
NheI G'CTAG_C
Cuts at: 0 969 3357
Size: 969 2388
NsiI A_TGCA'T
Cuts at: 0 640 1260 1900 2913 3357
Size: 640 620 640 1013 444
Fragments arranged by size:
1013 640 640 620 444
NspI r_CATG'y
Cuts at: 0 1262 1926 2911 3357
Size: 1262 664 985 446
Fragments arranged by size:
1262 985 664 446
PasI CC'CwG_GG
Cuts at: 0 51 843 3357
Size: 51 792 2514
Fragments arranged by size:
2514 792 51
PfoI T'CCnGG_A
Cuts at: 0 3170 3357
Size: 3170 187
PspOMI G'GGCC_C
Cuts at: 0 1272 3357
Size: 1272 2085
PstI C_TGCA'G
Cuts at: 0 213 2492 3357
Size: 213 2279 865
Fragments arranged by size:
2279 865 213
PvuII CAG'CTG
Cuts at: 0 502 3357
Size: 502 2855
SacI G_AGCT'C
Cuts at: 0 2154 3191 3357
Size: 2154 1037 166
Fragments arranged by size:
2154 1037 166
SapI GCTCTTCn'nnn_
Cuts at: 0 659 3357
Size: 659 2698
SfcI C'TryA_G
Cuts at: 0 209 314 2488 2618 3357
Size: 209 105 2174 130 739
Fragments arranged by size:
2174 739 209 130 105
SfoI GGC'GCC
Cuts at: 0 28 3357
Size: 28 3329
SmlI C'TyrA_G
Cuts at: 0 106 451 1825 3349 3357
Size: 106 345 1374 1524 8
Fragments arranged by size:
1524 1374 345 106 8
SpeI A'CTAG_T
Cuts at: 0 2263 3357
Size: 2263 1094
SspI AAT'ATT
Cuts at: 0 1068 2116 2837 3131 3357
Size: 1068 1048 721 294 226
Fragments arranged by size:
1068 1048 721 294 226
StuI AGG'CCT
Cuts at: 0 1741 3357
Size: 1741 1616
StyI C'CwwG_G
Cuts at: 0 1565 3357
Size: 1565 1792
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 856 1226 3357
Size: 856 370 2131
Fragments arranged by size:
2131 856 370
TatI w'GTAC_w
Cuts at: 0 1146 1240 3357
Size: 1146 94 2117
Fragments arranged by size:
2117 1146 94
Tth111I GACn'n_nGTC
Cuts at: 0 1720 3357
Size: 1720 1637
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 636 3357
Size: 636 2721
Enzymes that do cut:
AarI AccI AcuI AflII AleI AlfI ApaI ApaLI
ApoI AseI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BseRI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI
BsrGI BstAPI BstEII BstYI Bsu36I BtgI BtsI ClaI
CspCI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII HpaI KasI MfeI MmeI
MslI MspA1I NarI NcoI NdeI NheI NsiI NspI
PasI PfoI PspOMI PstI PvuII SacI SapI SfcI
SfoI SmlI SpeI SspI StuI StyI TaqII TatI
Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AloI
AlwNI AscI AsiSI AvaI AvrII BaeI BcgI BglI
BmgBI BsaAI BsaWI BsaXI BseYI BsiEI BsiWI BsmBI
BspEI BsrBI BssHII BssSI BstBI BstXI BstZ17I BtgZI
DraI DraIII DrdI EaeI EagI EciI FseI FspI
FspAI HindIII KpnI MluI MscI NaeI NgoMIV NotI
NruI PacI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SphI SrfI SwaI TstI XbaI XhoI XmaI
XmnI ZraI