Restriction map for
pF1KB7344
(Linear) (Six-base) MAPSORT of: KIBB7344.seq Check: 8913 from: 1 to: 3147
KIBB7344.orf
With 182 enzymes: SgfI *
September 8, 2016 13:38 ..
AccI GT'mk_AC
Cuts at: 0 1245 2712 3147
Size: 1245 1467 435
Fragments arranged by size:
1467 1245 435
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 1970 2560 3147
Size: 593 1377 590 587
Fragments arranged by size:
1377 593 590 587
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1939 3147
Size: 1939 1208
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 877 909 3147
Size: 877 32 2238
Fragments arranged by size:
2238 877 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1032 3147
Size: 1032 2115
ApaLI G'TGCA_C
Cuts at: 0 2551 3147
Size: 2551 596
ApoI r'AATT_y
Cuts at: 0 70 215 504 650 830 849 855 986
Size: 70 145 289 146 180 19 6 131
Cuts at: 986 1066 2034 2073 2260 3147
Size: 80 968 39 187 887
Fragments arranged by size:
968 887 289 187 180 146 145 131
80 70 39 19 6
AseI AT'TA_AT
Cuts at: 0 305 326 1436 3147
Size: 305 21 1110 1711
Fragments arranged by size:
1711 1110 305 21
AvaI C'yCGr_G
Cuts at: 0 862 2445 2821 3147
Size: 862 1583 376 326
Fragments arranged by size:
1583 862 376 326
AvrII C'CTAG_G
Cuts at: 0 2286 3147
Size: 2286 861
BanI G'GyrC_C
Cuts at: 0 109 2896 2941 2965 3147
Size: 109 2787 45 24 182
Fragments arranged by size:
2787 182 109 45 24
BanII G_rGCy'C
Cuts at: 0 387 428 2372 3147
Size: 387 41 1944 775
Fragments arranged by size:
1944 775 387 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 191 806 2228 2741 3147
Size: 191 615 1422 513 406
Fragments arranged by size:
1422 615 513 406 191
BciVI GTATCCnnnnn_n'
Cuts at: 0 2238 3147
Size: 2238 909
BclI T'GATC_A
Cuts at: 0 276 3147
Size: 276 2871
BfrBI ATG'CAT
Cuts at: 0 1877 3147
Size: 1877 1270
BglI GCCn_nnn'nGGC
Cuts at: 0 2939 3147
Size: 2939 208
BglII A'GATC_T
Cuts at: 0 1929 3147
Size: 1929 1218
Bme1580I G_kGCm'C
Cuts at: 0 2555 2970 3147
Size: 2555 415 177
Fragments arranged by size:
2555 415 177
BmrI ACTGGGnnnn_n'
Cuts at: 0 1141 2912 3147
Size: 1141 1771 235
Fragments arranged by size:
1771 1141 235
BmtI G_CTAG'C
Cuts at: 0 1300 3147
Size: 1300 1847
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 284 446 665 3147
Size: 27 257 162 219 2482
Fragments arranged by size:
2482 257 219 162 27
Bpu10I CC'TnA_GC
Cuts at: 0 2468 3007 3147
Size: 2468 539 140
Fragments arranged by size:
2468 539 140
BsaBI GATnn'nnATC
Cuts at: 0 1406 3147
Size: 1406 1741
BsaHI Gr'CG_yC
Cuts at: 0 2133 3147
Size: 2133 1014
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1205 1235 3147
Size: 1205 30 1912
Fragments arranged by size:
1912 1205 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1648 1718 1952 1955 3060 3147
Size: 1648 70 234 3 1105 87
Fragments arranged by size:
1648 1105 234 87 70 3
BseYI C'CCAG_C
Cuts at: 0 2956 3147
Size: 2956 191
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1757 3147
Size: 1757 1390
BsiHKAI G_wGCw'C
Cuts at: 0 387 428 1728 2437 2555 3147
Size: 387 41 1300 709 118 592
Fragments arranged by size:
1300 709 592 387 118 41
BsmI GAATG_Cn'
Cuts at: 0 123 1879 1997 3147
Size: 123 1756 118 1150
Fragments arranged by size:
1756 1150 123 118
Bsp1286I G_dGCh'C
Cuts at: 0 387 428 1728 2372 2437 2555 2970 3147
Size: 387 41 1300 644 65 118 415 177
Fragments arranged by size:
1300 644 415 387 177 118 65 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1301 3147
Size: 1301 1846
BsrBI CCG'CTC
Cuts at: 0 2963 3147
Size: 2963 184
BsrDI GCAATG_nn'
Cuts at: 0 35 3147
Size: 35 3112
BsrGI T'GTAC_A
Cuts at: 0 1629 3147
Size: 1629 1518
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1032 3147
Size: 1032 2115
BstBI TT'CG_AA
Cuts at: 0 654 3147
Size: 654 2493
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3079 3147
Size: 3079 68
BstYI r'GATC_y
Cuts at: 0 293 1929 3147
Size: 293 1636 1218
Fragments arranged by size:
1636 1218 293
Bsu36I CC'TnA_GG
Cuts at: 0 2357 3147
Size: 2357 790
BtsI GCAGTG_nn'
Cuts at: 0 2934 3147
Size: 2934 213
DraI TTT'AAA
Cuts at: 0 992 3147
Size: 992 2155
EaeI y'GGCC_r
Cuts at: 0 690 2323 3036 3147
Size: 690 1633 713 111
Fragments arranged by size:
1633 713 690 111
EarI CTCTTCn'nnn_
Cuts at: 0 884 3147
Size: 884 2263
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3075 3147
Size: 3075 72
EcoICRI GAG'CTC
Cuts at: 0 385 426 3147
Size: 385 41 2721
Fragments arranged by size:
2721 385 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 284 446 593 665 1970 2560 3147
Size: 27 257 162 147 72 1305 590 587
Fragments arranged by size:
1305 590 587 257 162 147 72 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2356 3147
Size: 2356 791
EcoO109I rG'GnC_Cy
Cuts at: 0 2254 2449 3147
Size: 2254 195 698
Fragments arranged by size:
2254 698 195
EcoRI G'AATT_C
Cuts at: 0 70 3147
Size: 70 3077
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1547 3147
Size: 1547 1600
HaeII r_GCGC'y
Cuts at: 0 2624 3147
Size: 2624 523
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 730 762 1603 1635 1646 1661 1678 1693
Size: 730 32 841 32 11 15 17 15
Cuts at: 1693 2424 2456 2718 2750 2790 2822 3147
Size: 731 32 262 32 40 32 325
Fragments arranged by size:
841 731 730 325 262 40 32 32
32 32 32 17 15 15 11
HincII GTy'rAC
Cuts at: 0 63 3147
Size: 63 3084
HpaI GTT'AAC
Cuts at: 0 63 3147
Size: 63 3084
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2805 3147
Size: 2805 342
MscI TGG'CCA
Cuts at: 0 2325 3038 3147
Size: 2325 713 109
Fragments arranged by size:
2325 713 109
MslI CAynn'nnrTG
Cuts at: 0 2947 3147
Size: 2947 200
MspA1I CmG'CkG
Cuts at: 0 751 1288 1741 2912 3147
Size: 751 537 453 1171 235
Fragments arranged by size:
1171 751 537 453 235
NdeI CA'TA_TG
Cuts at: 0 288 1173 2106 3147
Size: 288 885 933 1041
Fragments arranged by size:
1041 933 885 288
NheI G'CTAG_C
Cuts at: 0 1296 3147
Size: 1296 1851
NsiI A_TGCA'T
Cuts at: 0 1879 3147
Size: 1879 1268
NspI r_CATG'y
Cuts at: 0 747 3147
Size: 747 2400
PasI CC'CwG_GG
Cuts at: 0 1817 2250 3147
Size: 1817 433 897
Fragments arranged by size:
1817 897 433
PflMI CCAn_nnn'nTGG
Cuts at: 0 119 289 2617 3147
Size: 119 170 2328 530
Fragments arranged by size:
2328 530 170 119
PpuMI rG'GwC_Cy
Cuts at: 0 2254 2449 3147
Size: 2254 195 698
Fragments arranged by size:
2254 698 195
PsiI TTA'TAA
Cuts at: 0 95 595 3147
Size: 95 500 2552
Fragments arranged by size:
2552 500 95
PvuII CAG'CTG
Cuts at: 0 1288 1741 3147
Size: 1288 453 1406
Fragments arranged by size:
1406 1288 453
SacI G_AGCT'C
Cuts at: 0 387 428 3147
Size: 387 41 2719
Fragments arranged by size:
2719 387 41
ScaI AGT'ACT
Cuts at: 0 1527 3147
Size: 1527 1620
SexAI A'CCwGG_T
Cuts at: 0 2451 3147
Size: 2451 696
SfcI C'TryA_G
Cuts at: 0 1397 3147
Size: 1397 1750
SmlI C'TyrA_G
Cuts at: 0 862 3147
Size: 862 2285
SspI AAT'ATT
Cuts at: 0 1440 3147
Size: 1440 1707
StuI AGG'CCT
Cuts at: 0 417 3147
Size: 417 2730
StyI C'CwwG_G
Cuts at: 0 1346 1380 2286 3147
Size: 1346 34 906 861
Fragments arranged by size:
1346 906 861 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 737 2402 2527 2941 3147
Size: 737 1665 125 414 206
Fragments arranged by size:
1665 737 414 206 125
TatI w'GTAC_w
Cuts at: 0 1355 1525 1629 2457 3147
Size: 1355 170 104 828 690
Fragments arranged by size:
1355 828 690 170 104
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 745 777 2898 2930 3147
Size: 745 32 2121 32 217
Fragments arranged by size:
2121 745 217 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2514 3147
Size: 2514 633
XbaI T'CTAG_A
Cuts at: 0 420 672 3147
Size: 420 252 2475
Fragments arranged by size:
2475 420 252
XhoI C'TCGA_G
Cuts at: 0 862 3147
Size: 862 2285
XmnI GAAnn'nnTTC
Cuts at: 0 2034 3147
Size: 2034 1113
Enzymes that do cut:
AccI AcuI AlfI AloI AlwNI ApaLI ApoI AseI
AvaI AvrII BanI BanII BbsI BciVI BclI BfrBI
BglI BglII Bme1580I BmrI BmtI BpmI Bpu10I BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrDI BsrGI BstAPI BstBI BstXI BstYI
Bsu36I BtsI DraI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HpaI
MmeI MscI MslI MspA1I NdeI NheI NsiI NspI
PasI PflMI PpuMI PsiI PvuII SacI ScaI SexAI
SfcI SmlI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI ApaI AscI AsiSI BaeI BamHI BbeI
BbvCI BcgI BlpI BmgBI BplI BpuEI BsaI BsaAI
BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrFI BssHII
BssSI BstEII BstZ17I BtgI BtgZI ClaI CspCI DraIII
DrdI EagI EcoRV FseI FspI FspAI HindIII KasI
KpnI MfeI MluI NaeI NarI NcoI NgoMIV NotI
NruI PacI PciI PfoI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SwaI XcmI XmaI ZraI