Restriction map for
pF1KB7342
(Linear) (Six-base) MAPSORT of: KIBB7342.seq Check: 945 from: 1 to: 2682
KIBB7342.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 0 1172 2682
Size: 1172 1510
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 189 761 2234 2682
Size: 106 83 572 1473 448
Fragments arranged by size:
1473 572 448 106 83
AflII C'TTAA_G
Cuts at: 0 1515 2682
Size: 1515 1167
AflIII A'CryG_T
Cuts at: 0 459 2128 2682
Size: 459 1669 554
Fragments arranged by size:
1669 554 459
AgeI A'CCGG_T
Cuts at: 0 898 2682
Size: 898 1784
AhdI GACnn_n'nnGTC
Cuts at: 0 1748 2682
Size: 1748 934
ApaI G_GGCC'C
Cuts at: 0 1405 2682
Size: 1405 1277
ApaLI G'TGCA_C
Cuts at: 0 1359 2682
Size: 1359 1323
ApoI r'AATT_y
Cuts at: 0 209 1612 2193 2647 2682
Size: 209 1403 581 454 35
Fragments arranged by size:
1403 581 454 209 35
AseI AT'TA_AT
Cuts at: 0 1559 2682
Size: 1559 1123
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 887 920 2682
Size: 887 33 1762
Fragments arranged by size:
1762 887 33
BanII G_rGCy'C
Cuts at: 0 411 1342 1405 2682
Size: 411 931 63 1277
Fragments arranged by size:
1277 931 411 63
BbsI GAAGACnn'nnnn_
Cuts at: 0 248 2183 2537 2682
Size: 248 1935 354 145
Fragments arranged by size:
1935 354 248 145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 604 638 2682
Size: 604 34 2044
Fragments arranged by size:
2044 604 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1468 2682
Size: 1468 1214
BclI T'GATC_A
Cuts at: 0 683 2682
Size: 683 1999
BfrBI ATG'CAT
Cuts at: 0 2672 2682
Size: 2672 10
Bme1580I G_kGCm'C
Cuts at: 0 1363 1405 2682
Size: 1363 42 1277
Fragments arranged by size:
1363 1277 42
BmgBI CAC'GTC
Cuts at: 0 1107 2682
Size: 1107 1575
BmrI ACTGGGnnnn_n'
Cuts at: 0 581 2682
Size: 581 2101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 1403 2243 2641 2682
Size: 175 1228 840 398 41
Fragments arranged by size:
1228 840 398 175 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 370 1051 1599 2682
Size: 151 219 681 548 1083
Fragments arranged by size:
1083 681 548 219 151
BsaWI w'CCGG_w
Cuts at: 0 185 769 898 2682
Size: 185 584 129 1784
Fragments arranged by size:
1784 584 185 129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 424 454 2216 2246 2682
Size: 424 30 1762 30 436
Fragments arranged by size:
1762 436 424 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 1145 2527 2682
Size: 172 973 1382 155
Fragments arranged by size:
1382 973 172 155
BseYI C'CCAG_C
Cuts at: 0 1452 2186 2682
Size: 1452 734 496
Fragments arranged by size:
1452 734 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 2682
Size: 470 2212
BsiEI CG_ry'CG
Cuts at: 0 2601 2682
Size: 2601 81
BsiHKAI G_wGCw'C
Cuts at: 0 1342 1363 2682
Size: 1342 21 1319
Fragments arranged by size:
1342 1319 21
BsmI GAATG_Cn'
Cuts at: 0 1414 2682
Size: 1414 1268
BsmBI CGTCTCn'nnnn_
Cuts at: 0 294 393 2036 2682
Size: 294 99 1643 646
Fragments arranged by size:
1643 646 294 99
Bsp1286I G_dGCh'C
Cuts at: 0 411 1342 1363 1405 2682
Size: 411 931 21 42 1277
Fragments arranged by size:
1277 931 411 42 21
BspEI T'CCGG_A
Cuts at: 0 769 2682
Size: 769 1913
BsrFI r'CCGG_y
Cuts at: 0 898 2575 2682
Size: 898 1677 107
Fragments arranged by size:
1677 898 107
BsrGI T'GTAC_A
Cuts at: 0 586 2682
Size: 586 2096
BstAPI GCAn_nnn'nTGC
Cuts at: 0 646 1657 2682
Size: 646 1011 1025
Fragments arranged by size:
1025 1011 646
BstYI r'GATC_y
Cuts at: 0 470 1221 1788 2682
Size: 470 751 567 894
Fragments arranged by size:
894 751 567 470
BstZ17I GTA'TAC
Cuts at: 0 1173 2682
Size: 1173 1509
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 95 2682
Size: 95 2587
BtsI GCAGTG_nn'
Cuts at: 0 1658 2141 2682
Size: 1658 483 541
Fragments arranged by size:
1658 541 483
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 662 697 786 821 2364 2399 2682
Size: 662 35 89 35 1543 35 283
Fragments arranged by size:
1543 662 283 89 35 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 735 2682
Size: 735 1947
DrdI GACnn_nn'nnGTC
Cuts at: 0 2400 2682
Size: 2400 282
EaeI y'GGCC_r
Cuts at: 0 173 1458 1665 2310 2573 2682
Size: 173 1285 207 645 263 109
Fragments arranged by size:
1285 645 263 207 173 109
EarI CTCTTCn'nnn_
Cuts at: 0 355 2606 2682
Size: 355 2251 76
Fragments arranged by size:
2251 355 76
EcoICRI GAG'CTC
Cuts at: 0 1340 2682
Size: 1340 1342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 175 189 761 1403 2234 2243 2641
Size: 106 69 14 572 642 831 9 398
Cuts at: 2641 2682
Size: 41
Fragments arranged by size:
831 642 572 398 106 69 41 14
9
EcoO109I rG'GnC_Cy
Cuts at: 0 1401 1402 2218 2682
Size: 1401 1 816 464
Fragments arranged by size:
1401 816 464 1
EcoRI G'AATT_C
Cuts at: 0 209 2682
Size: 209 2473
EcoRV GAT'ATC
Cuts at: 0 579 2682
Size: 579 2103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 118 1486 2682
Size: 118 1368 1196
Fragments arranged by size:
1368 1196 118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 725 757 1591 1623 1734 1766 2682
Size: 725 32 834 32 111 32 916
Fragments arranged by size:
916 834 725 111 32 32 32
HincII GTy'rAC
Cuts at: 0 1897 1979 2682
Size: 1897 82 703
Fragments arranged by size:
1897 703 82
HpaI GTT'AAC
Cuts at: 0 1979 2682
Size: 1979 703
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2294 2396 2682
Size: 2294 102 286
Fragments arranged by size:
2294 286 102
MscI TGG'CCA
Cuts at: 0 1460 2312 2682
Size: 1460 852 370
Fragments arranged by size:
1460 852 370
MslI CAynn'nnrTG
Cuts at: 0 1700 2013 2287 2682
Size: 1700 313 274 395
Fragments arranged by size:
1700 395 313 274
MspA1I CmG'CkG
Cuts at: 0 1456 2682
Size: 1456 1226
NaeI GCC'GGC
Cuts at: 0 2577 2682
Size: 2577 105
NdeI CA'TA_TG
Cuts at: 0 1973 2682
Size: 1973 709
NgoMIV G'CCGG_C
Cuts at: 0 2575 2682
Size: 2575 107
NsiI A_TGCA'T
Cuts at: 0 2674 2682
Size: 2674 8
NspI r_CATG'y
Cuts at: 0 2132 2292 2672 2682
Size: 2132 160 380 10
Fragments arranged by size:
2132 380 160 10
PasI CC'CwG_GG
Cuts at: 0 22 2682
Size: 22 2660
PciI A'CATG_T
Cuts at: 0 2128 2682
Size: 2128 554
PpuMI rG'GwC_Cy
Cuts at: 0 2218 2682
Size: 2218 464
PsiI TTA'TAA
Cuts at: 0 1916 2682
Size: 1916 766
PspOMI G'GGCC_C
Cuts at: 0 1401 2682
Size: 1401 1281
PstI C_TGCA'G
Cuts at: 0 651 2596 2682
Size: 651 1945 86
Fragments arranged by size:
1945 651 86
PvuI CG_AT'CG
Cuts at: 0 2601 2682
Size: 2601 81
PvuII CAG'CTG
Cuts at: 0 1456 2682
Size: 1456 1226
SacI G_AGCT'C
Cuts at: 0 1342 2682
Size: 1342 1340
ScaI AGT'ACT
Cuts at: 0 1595 2682
Size: 1595 1087
SfcI C'TryA_G
Cuts at: 0 647 1784 2592 2682
Size: 647 1137 808 90
Fragments arranged by size:
1137 808 647 90
SmlI C'TyrA_G
Cuts at: 0 166 385 1030 1515 1578 2682
Size: 166 219 645 485 63 1104
Fragments arranged by size:
1104 645 485 219 166 63
SspI AAT'ATT
Cuts at: 0 1638 2247 2682
Size: 1638 609 435
Fragments arranged by size:
1638 609 435
StyI C'CwwG_G
Cuts at: 0 1470 2396 2682
Size: 1470 926 286
Fragments arranged by size:
1470 926 286
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 905 970 2482 2682
Size: 905 65 1512 200
Fragments arranged by size:
1512 905 200 65
TatI w'GTAC_w
Cuts at: 0 327 586 1593 2035 2682
Size: 327 259 1007 442 647
Fragments arranged by size:
1007 647 442 327 259
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 764 796 2682
Size: 764 32 1886
Fragments arranged by size:
1886 764 32
XbaI T'CTAG_A
Cuts at: 0 213 2628 2682
Size: 213 2415 54
Fragments arranged by size:
2415 213 54
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1468 2476 2682
Size: 1468 1008 206
Fragments arranged by size:
1468 1008 206
Enzymes that do cut:
AccI AcuI AflII AflIII AgeI AhdI ApaI ApaLI
ApoI AseI BaeI BanII BbsI BcgI BciVI BclI
BfrBI Bme1580I BmgBI BmrI BpmI BpuEI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BsrFI BsrGI BstAPI BstYI BstZ17I BtgZI BtsI
CspCI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI Hin4I HincII HpaI MmeI MscI
MslI MspA1I NaeI NdeI NgoMIV NsiI NspI PasI
PciI PpuMI PsiI PspOMI PstI PvuI PvuII SacI
ScaI SfcI SmlI SspI StyI TaqII TatI TstI
XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AleI AlfI AloI
AlwNI AscI AsiSI AvaI AvrII BamHI BanI BbeI
BbvCI BglI BglII BlpI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaHI BsiWI BspHI BspMI BsrBI BsrDI
BssHII BssSI BstBI BstEII BstXI Bsu36I BtgI ClaI
DraI EagI EciI EcoNI FseI FspI FspAI HaeII
HindIII KasI KpnI MfeI MluI NarI NcoI NheI
NotI NruI PacI PflMI PfoI PmeI PmlI PpiI
PshAI PspXI PsrI RsrII SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI Tth111I XhoI XmaI
XmnI ZraI