Restriction map for
pF1KB7341
(Linear) (Six-base) MAPSORT of: KIBB7341.seq Check: 2405 from: 1 to: 5532
KIBB7341.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 0 887 3492 3539 4346 5267 5532
Size: 887 2605 47 807 921 265
Fragments arranged by size:
2605 921 887 807 265 47
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 779 1184 1432 1763 2057 2183 2293 2840
Size: 779 405 248 331 294 126 110 547
Cuts at: 2840 3167 3304 3890 4436 5532
Size: 327 137 586 546 1096
Fragments arranged by size:
1096 779 586 547 546 405 331 327
294 248 137 126 110
AflII C'TTAA_G
Cuts at: 0 2181 4393 5532
Size: 2181 2212 1139
Fragments arranged by size:
2212 2181 1139
AflIII A'CryG_T
Cuts at: 0 2613 4341 4964 5532
Size: 2613 1728 623 568
Fragments arranged by size:
2613 1728 623 568
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4390 4422 5532
Size: 4390 32 1110
Fragments arranged by size:
4390 1110 32
AlwNI CAG_nnn'CTG
Cuts at: 0 271 967 3282 4370 4600 5532
Size: 271 696 2315 1088 230 932
Fragments arranged by size:
2315 1088 932 696 271 230
ApoI r'AATT_y
Cuts at: 0 558 678 805 935 1022 1344 1972 2683
Size: 558 120 127 130 87 322 628 711
Cuts at: 2683 2743 2757 2812 2869 3012 3497 3792 4119
Size: 60 14 55 57 143 485 295 327
Cuts at: 4119 4313 4471 4492 4938 5193 5477 5532
Size: 194 158 21 446 255 284 55
Fragments arranged by size:
711 628 558 485 446 327 322 295
284 255 194 158 143 130 127 120
87 60 57 55 55 21 14
AseI AT'TA_AT
Cuts at: 0 1930 5532
Size: 1930 3602
AvaI C'yCGr_G
Cuts at: 0 3318 5532
Size: 3318 2214
BanII G_rGCy'C
Cuts at: 0 1960 5532
Size: 1960 3572
BbsI GAAGACnn'nnnn_
Cuts at: 0 148 229 434 1256 3070 3155 4424 5532
Size: 148 81 205 822 1814 85 1269 1108
Fragments arranged by size:
1814 1269 1108 822 205 148 85 81
BciVI GTATCCnnnnn_n'
Cuts at: 0 1363 4198 5532
Size: 1363 2835 1334
Fragments arranged by size:
2835 1363 1334
BclI T'GATC_A
Cuts at: 0 4758 5532
Size: 4758 774
BglI GCCn_nnn'nGGC
Cuts at: 0 22 5532
Size: 22 5510
BglII A'GATC_T
Cuts at: 0 642 1786 2023 2712 5532
Size: 642 1144 237 689 2820
Fragments arranged by size:
2820 1144 689 642 237
Bme1580I G_kGCm'C
Cuts at: 0 3438 5532
Size: 3438 2094
BmrI ACTGGGnnnn_n'
Cuts at: 0 762 1472 5532
Size: 762 710 4060
Fragments arranged by size:
4060 762 710
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5363 5532
Size: 5363 169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2980 3079 5135 5532
Size: 2980 99 2056 397
Fragments arranged by size:
2980 2056 397 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2863 4552 5101 5532
Size: 2863 1689 549 431
Fragments arranged by size:
2863 1689 549 431
BsaI GGTCTCn'nnnn_
Cuts at: 0 4372 5532
Size: 4372 1160
BsaBI GATnn'nnATC
Cuts at: 0 4727 5532
Size: 4727 805
BsaWI w'CCGG_w
Cuts at: 0 4816 5532
Size: 4816 716
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4211 4241 5532
Size: 4211 30 1291
Fragments arranged by size:
4211 1291 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2181 3928 5532
Size: 2181 1747 1604
Fragments arranged by size:
2181 1747 1604
BseYI C'CCAG_C
Cuts at: 0 941 3479 3877 3943 5532
Size: 941 2538 398 66 1589
Fragments arranged by size:
2538 1589 941 398 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4634 5446 5532
Size: 4634 812 86
Fragments arranged by size:
4634 812 86
BsiHKAI G_wGCw'C
Cuts at: 0 2921 3693 5532
Size: 2921 772 1839
Fragments arranged by size:
2921 1839 772
BsmI GAATG_Cn'
Cuts at: 0 3474 3767 3960 5532
Size: 3474 293 193 1572
Fragments arranged by size:
3474 1572 293 193
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4814 5532
Size: 4814 718
Bsp1286I G_dGCh'C
Cuts at: 0 1960 2921 3438 3693 5532
Size: 1960 961 517 255 1839
Fragments arranged by size:
1960 1839 961 517 255
BspHI T'CATG_A
Cuts at: 0 2795 5532
Size: 2795 2737
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 112 936 5399 5532
Size: 112 824 4463 133
Fragments arranged by size:
4463 824 133 112
BsrGI T'GTAC_A
Cuts at: 0 1848 2576 4275 5532
Size: 1848 728 1699 1257
Fragments arranged by size:
1848 1699 1257 728
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1237 3427 5532
Size: 1237 2190 2105
Fragments arranged by size:
2190 2105 1237
BstBI TT'CG_AA
Cuts at: 0 5403 5532
Size: 5403 129
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3445 3487 3708 5532
Size: 3445 42 221 1824
Fragments arranged by size:
3445 1824 221 42
BstYI r'GATC_y
Cuts at: 0 642 1786 2023 2712 5217 5532
Size: 642 1144 237 689 2505 315
Fragments arranged by size:
2505 1144 689 642 315 237
BstZ17I GTA'TAC
Cuts at: 0 5268 5532
Size: 5268 264
BtgI C'CryG_G
Cuts at: 0 17 5532
Size: 17 5515
BtsI GCAGTG_nn'
Cuts at: 0 2591 3428 3492 5532
Size: 2591 837 64 2040
Fragments arranged by size:
2591 2040 837 64
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3386 3421 5532
Size: 3386 35 2111
Fragments arranged by size:
3386 2111 35
DraI TTT'AAA
Cuts at: 0 440 812 2279 2688 3336 3723 4436 4937
Size: 440 372 1467 409 648 387 713 501
Cuts at: 4937 5532
Size: 595
Fragments arranged by size:
1467 713 648 595 501 440 409 387
372
DrdI GACnn_nn'nnGTC
Cuts at: 0 895 5532
Size: 895 4637
EaeI y'GGCC_r
Cuts at: 0 14 5532
Size: 14 5518
EarI CTCTTCn'nnn_
Cuts at: 0 833 1308 3111 3249 3353 3480 5208 5302
Size: 833 475 1803 138 104 127 1728 94
Cuts at: 5302 5354 5532
Size: 52 178
Fragments arranged by size:
1803 1728 833 475 178 138 127 104
94 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 26 983 5532
Size: 26 957 4549
Fragments arranged by size:
4549 957 26
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 779 1184 1432 1763 2057 2183 2293 2840
Size: 779 405 248 331 294 126 110 547
Cuts at: 2840 2980 3079 3167 3304 3890 4436 5135 5532
Size: 140 99 88 137 586 546 699 397
Fragments arranged by size:
779 699 586 547 546 405 397 331
294 248 140 137 126 110 99 88
EcoNI CCTnn'n_nnAGG
Cuts at: 0 581 1648 5532
Size: 581 1067 3884
Fragments arranged by size:
3884 1067 581
EcoO109I rG'GnC_Cy
Cuts at: 0 9 5532
Size: 9 5523
EcoRI G'AATT_C
Cuts at: 0 2757 4492 5193 5477 5532
Size: 2757 1735 701 284 55
Fragments arranged by size:
2757 1735 701 284 55
EcoRV GAT'ATC
Cuts at: 0 4176 5315 5532
Size: 4176 1139 217
Fragments arranged by size:
4176 1139 217
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 482 1610 1929 2102 2523 3172 5532
Size: 482 1128 319 173 421 649 2360
Fragments arranged by size:
2360 1128 649 482 421 319 173
HaeII r_GCGC'y
Cuts at: 0 2190 5532
Size: 2190 3342
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 82 114 3980 4012 4951 4983 5336 5368
Size: 82 32 3866 32 939 32 353 32
Cuts at: 5368 5532
Size: 164
Fragments arranged by size:
3866 939 353 164 82 32 32 32
32
HincII GTy'rAC
Cuts at: 0 276 532 888 1778 1864 4157 5532
Size: 276 256 356 890 86 2293 1375
Fragments arranged by size:
2293 1375 890 356 276 256 86
HindIII A'AGCT_T
Cuts at: 0 654 1717 2132 5532
Size: 654 1063 415 3400
Fragments arranged by size:
3400 1063 654 415
HpaI GTT'AAC
Cuts at: 0 4157 5532
Size: 4157 1375
MfeI C'AATT_G
Cuts at: 0 398 2113 5532
Size: 398 1715 3419
Fragments arranged by size:
3419 1715 398
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3843 5014 5245 5532
Size: 3843 1171 231 287
Fragments arranged by size:
3843 1171 287 231
MscI TGG'CCA
Cuts at: 0 16 5532
Size: 16 5516
MspA1I CmG'CkG
Cuts at: 0 31 2419 5131 5521 5532
Size: 31 2388 2712 390 11
Fragments arranged by size:
2712 2388 390 31 11
NcoI C'CATG_G
Cuts at: 0 17 5532
Size: 17 5515
NdeI CA'TA_TG
Cuts at: 0 798 5532
Size: 798 4734
NspI r_CATG'y
Cuts at: 0 2617 3156 4968 5532
Size: 2617 539 1812 564
Fragments arranged by size:
2617 1812 564 539
PciI A'CATG_T
Cuts at: 0 2613 4964 5532
Size: 2613 2351 568
Fragments arranged by size:
2613 2351 568
PpuMI rG'GwC_Cy
Cuts at: 0 9 5532
Size: 9 5523
PsiI TTA'TAA
Cuts at: 0 2251 3388 5532
Size: 2251 1137 2144
Fragments arranged by size:
2251 2144 1137
PstI C_TGCA'G
Cuts at: 0 30 3423 4902 5136 5532
Size: 30 3393 1479 234 396
Fragments arranged by size:
3393 1479 396 234 30
PvuII CAG'CTG
Cuts at: 0 31 2419 5131 5521 5532
Size: 31 2388 2712 390 11
Fragments arranged by size:
2712 2388 390 31 11
SalI G'TCGA_C
Cuts at: 0 886 5532
Size: 886 4646
SapI GCTCTTCn'nnn_
Cuts at: 0 3249 5532
Size: 3249 2283
ScaI AGT'ACT
Cuts at: 0 465 1989 2927 4065 5532
Size: 465 1524 938 1138 1467
Fragments arranged by size:
1524 1467 1138 938 465
SfcI C'TryA_G
Cuts at: 0 26 432 1814 3419 4898 5132 5532
Size: 26 406 1382 1605 1479 234 400
Fragments arranged by size:
1605 1479 1382 406 400 234 26
SmlI C'TyrA_G
Cuts at: 0 2181 2842 4393 4531 5080 5532
Size: 2181 661 1551 138 549 452
Fragments arranged by size:
2181 1551 661 549 452 138
SpeI A'CTAG_T
Cuts at: 0 4281 5532
Size: 4281 1251
SspI AAT'ATT
Cuts at: 0 124 183 375 2887 4715 5532
Size: 124 59 192 2512 1828 817
Fragments arranged by size:
2512 1828 817 192 124 59
StyI C'CwwG_G
Cuts at: 0 17 662 3707 5532
Size: 17 645 3045 1825
Fragments arranged by size:
3045 1825 645 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 225 2624 5532
Size: 225 2399 2908
Fragments arranged by size:
2908 2399 225
TatI w'GTAC_w
Cuts at: 0 463 1848 1987 2576 2925 4063 4275 5532
Size: 463 1385 139 589 349 1138 212 1257
Fragments arranged by size:
1385 1257 1138 589 463 349 212 139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2620 2652 2839 2871 4200 4232 5532
Size: 2620 32 187 32 1329 32 1300
Fragments arranged by size:
2620 1329 1300 187 32 32 32
XbaI T'CTAG_A
Cuts at: 0 742 1188 2194 5532
Size: 742 446 1006 3338
Fragments arranged by size:
3338 1006 742 446
XmnI GAAnn'nnTTC
Cuts at: 0 204 512 5477 5532
Size: 204 308 4965 55
Fragments arranged by size:
4965 308 204 55
Enzymes that do cut:
AccI AcuI AflII AflIII AloI AlwNI ApoI AseI
AvaI BanII BbsI BciVI BclI BglI BglII Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrGI BstAPI BstBI BstXI BstYI BstZ17I BtgI
BtsI CspCI DraI DrdI EaeI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
HindIII HpaI MfeI MmeI MscI MspA1I NcoI NdeI
NspI PciI PpuMI PsiI PstI PvuII SalI SapI
ScaI SfcI SmlI SpeI SspI StyI TaqII TatI
TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BcgI BfrBI BlpI BmgBI BmtI
Bpu10I BsaAI BsaHI BsiEI BsiWI BspEI BsrBI BsrDI
BsrFI BssHII BssSI BstEII Bsu36I BtgZI ClaI DraIII
EagI EcoICRI FseI FspI FspAI KasI KpnI MluI
MslI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI StuI SwaI Tth111I XcmI XhoI XmaI
ZraI