(Linear) (Six-base) MAPSORT of: KIBB7270.seq  Check: 9165  from: 1  to: 639



KIBB7270.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     639 
   Size:         97     542
 
AlwNI CAG_nnn'CTG

Cuts at:      0     187     580     639 
   Size:        187     393      59

  Fragments arranged by size: 

                 393     187      59
 
ApaI G_GGCC'C

Cuts at:      0      32     639 
   Size:         32     607
 
ApoI r'AATT_y

Cuts at:      0     513     626     639 
   Size:        513     113      13

  Fragments arranged by size: 

                 513     113      13
 
AvaI C'yCGr_G

Cuts at:      0      24     639 
   Size:         24     615
 
AvrII C'CTAG_G

Cuts at:      0      70     639 
   Size:         70     569
 
BanII G_rGCy'C

Cuts at:      0      32     145     639 
   Size:         32     113     494

  Fragments arranged by size: 

                 494     113      32
 
Bme1580I G_kGCm'C

Cuts at:      0      32     639 
   Size:         32     607
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     374     395     639 
   Size:        374      21     244

  Fragments arranged by size: 

                 374     244      21
 
Bpu10I CC'TnA_GC

Cuts at:      0       5     639 
   Size:          5     634
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     489     639 
   Size:        489     150
 
Bsp1286I G_dGCh'C

Cuts at:      0      32     145     639 
   Size:         32     113     494

  Fragments arranged by size: 

                 494     113      32
 
BsrGI T'GTAC_A

Cuts at:      0     195     639 
   Size:        195     444
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     580     639 
   Size:        580      59
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     639 
   Size:         97     542
 
EcoO109I rG'GnC_Cy

Cuts at:      0      28     639 
   Size:         28     611
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     127     502     639 
   Size:        127     375     137

  Fragments arranged by size: 

                 375     137     127
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     175     207     639 
   Size:        175      32     432

  Fragments arranged by size: 

                 432     175      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     191     639 
   Size:        191     448
 
PspOMI G'GGCC_C

Cuts at:      0      28     639 
   Size:         28     611
 
PstI C_TGCA'G

Cuts at:      0     585     639 
   Size:        585      54
 
ScaI AGT'ACT

Cuts at:      0     405     639 
   Size:        405     234
 
SfcI C'TryA_G

Cuts at:      0     581     639 
   Size:        581      58
 
SmaI CCC'GGG

Cuts at:      0      26     639 
   Size:         26     613
 
StyI C'CwwG_G

Cuts at:      0      70     639 
   Size:         70     569
 
TatI w'GTAC_w

Cuts at:      0     195     403     639 
   Size:        195     208     236

  Fragments arranged by size: 

                 236     208     195
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     144     176     639 
   Size:        144      32     463

  Fragments arranged by size: 

                 463     144      32
 
XmaI C'CCGG_G

Cuts at:      0      24     639 
   Size:         24     615
 
XmnI GAAnn'nnTTC

Cuts at:      0      87     110     626     639 
   Size:         87      23     516      13

  Fragments arranged by size: 

                 516      87      23      13
 

 Enzymes that do cut:

     AcuI    AlwNI     ApaI     ApoI     AvaI    AvrII    BanII Bme1580I
     BmrI   Bpu10I     BsgI Bsp1286I    BsrGI   BstAPI  Eco57MI EcoO109I
     FalI    Hin4I     MmeI   PspOMI     PstI     ScaI     SfcI     SmaI
     StyI     TatI     TstI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI
    AsiSI     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     ZraI