(Linear) (Six-base) MAPSORT of: KIBB7266.seq  Check: 233  from: 1  to: 1032



KIBB7266.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AccI GT'mk_AC

Cuts at:      0     995    1032 
   Size:        995      37
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     797     822    1032 
   Size:        283     514      25     210

  Fragments arranged by size: 

                 514     283     210      25
 
AleI CACnn'nnGTG

Cuts at:      0     554    1032 
   Size:        554     478
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      52     785    1032 
   Size:         52     733     247

  Fragments arranged by size: 

                 733     247      52
 
AlwNI CAG_nnn'CTG

Cuts at:      0     669    1032 
   Size:        669     363
 
ApoI r'AATT_y

Cuts at:      0     142     289     955    1032 
   Size:        142     147     666      77

  Fragments arranged by size: 

                 666     147     142      77
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     277     393    1032 
   Size:        277     116     639

  Fragments arranged by size: 

                 639     277     116
 
BbvCI CC'TCA_GC

Cuts at:      0     813    1032 
   Size:        813     219
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     750    1032 
   Size:        750     282
 
Bme1580I G_kGCm'C

Cuts at:      0     481    1032 
   Size:        481     551
 
BmgBI CAC'GTC

Cuts at:      0     399    1032 
   Size:        399     633
 
Bpu10I CC'TnA_GC

Cuts at:      0     562     813    1032 
   Size:        562     251     219

  Fragments arranged by size: 

                 562     251     219
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     668    1032 
   Size:        668     364
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     170    1032 
   Size:        170     862
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     517     530     956    1032 
   Size:        517      13     426      76

  Fragments arranged by size: 

                 517     426      76      13
 
Bsp1286I G_dGCh'C

Cuts at:      0     481    1032 
   Size:        481     551
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     418     590    1032 
   Size:        418     172     442

  Fragments arranged by size: 

                 442     418     172
 
BsrDI GCAATG_nn'

Cuts at:      0     569     923    1032 
   Size:        569     354     109

  Fragments arranged by size: 

                 569     354     109
 
BsrFI r'CCGG_y

Cuts at:      0      46    1032 
   Size:         46     986
 
BssSI C'ACGA_G

Cuts at:      0     550    1032 
   Size:        550     482
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     934    1032 
   Size:        934      98
 
BstBI TT'CG_AA

Cuts at:      0     404    1032 
   Size:        404     628
 
BstYI r'GATC_y

Cuts at:      0     679     780    1032 
   Size:        679     101     252

  Fragments arranged by size: 

                 679     252     101
 
Bsu36I CC'TnA_GG

Cuts at:      0     876    1032 
   Size:        876     156
 
BtgI C'CryG_G

Cuts at:      0      63     533     750    1032 
   Size:         63     470     217     282

  Fragments arranged by size: 

                 470     282     217      63
 
EaeI y'GGCC_r

Cuts at:      0     162     512    1032 
   Size:        162     350     520

  Fragments arranged by size: 

                 520     350     162
 
EarI CTCTTCn'nnn_

Cuts at:      0     669    1032 
   Size:        669     363
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     797     822    1032 
   Size:        283     514      25     210

  Fragments arranged by size: 

                 514     283     210      25
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     640    1032 
   Size:        640     392
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      86     118     631     663    1032 
   Size:         86      32     513      32     369

  Fragments arranged by size: 

                 513     369      86      32      32
 
MscI TGG'CCA

Cuts at:      0     164     514    1032 
   Size:        164     350     518

  Fragments arranged by size: 

                 518     350     164
 
MslI CAynn'nnrTG

Cuts at:      0     554    1032 
   Size:        554     478
 
MspA1I CmG'CkG

Cuts at:      0     775    1032 
   Size:        775     257
 
NaeI GCC'GGC

Cuts at:      0      48    1032 
   Size:         48     984
 
NgoMIV G'CCGG_C

Cuts at:      0      46    1032 
   Size:         46     986
 
PfoI T'CCnGG_A

Cuts at:      0     662    1032 
   Size:        662     370
 
PsiI TTA'TAA

Cuts at:      0     628    1032 
   Size:        628     404
 
PstI C_TGCA'G

Cuts at:      0     587    1032 
   Size:        587     445
 
PvuII CAG'CTG

Cuts at:      0     775    1032 
   Size:        775     257
 
SbfI CC_TGCA'GG

Cuts at:      0     587    1032 
   Size:        587     445
 
SfcI C'TryA_G

Cuts at:      0     296     583    1032 
   Size:        296     287     449

  Fragments arranged by size: 

                 449     296     287
 
SmlI C'TyrA_G

Cuts at:      0     683    1032 
   Size:        683     349
 
SspI AAT'ATT

Cuts at:      0     635     696    1032 
   Size:        635      61     336

  Fragments arranged by size: 

                 635     336      61
 
StuI AGG'CCT

Cuts at:      0     428    1032 
   Size:        428     604
 
StyI C'CwwG_G

Cuts at:      0     971    1032 
   Size:        971      61
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     366     398    1032 
   Size:        366      32     634

  Fragments arranged by size: 

                 634     366      32
 
XbaI T'CTAG_A

Cuts at:      0     147    1008    1032 
   Size:        147     861      24

  Fragments arranged by size: 

                 861     147      24
 

 Enzymes that do cut:

     AccI     AcuI     AleI     AlfI    AlwNI     ApoI     BbsI    BbvCI
    BciVI Bme1580I    BmgBI   Bpu10I    BpuEI    BseRI     BsgI Bsp1286I
    BspMI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI   Bsu36I
     BtgI     EaeI     EarI  Eco57MI     FalI    Hin4I     MscI     MslI
   MspA1I     NaeI   NgoMIV     PfoI     PsiI     PstI    PvuII     SbfI
     SfcI     SmlI     SspI     StuI     StyI     TstI     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BcgI     BclI
    BfrBI     BglI    BglII     BlpI     BmrI     BmtI     BplI     BpmI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SwaI    TaqII     TatI  Tth111I
     XcmI     XhoI     XmaI     XmnI     ZraI