(Linear) (Six-base) MAPSORT of: KIBB7264.seq  Check: 3431  from: 1  to: 747



KIBB7264.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     743     747 
   Size:        743       4
 
AflII C'TTAA_G

Cuts at:      0     138     196     747 
   Size:        138      58     551

  Fragments arranged by size: 

                 551     138      58
 
ApoI r'AATT_y

Cuts at:      0     118     133     279     747 
   Size:        118      15     146     468

  Fragments arranged by size: 

                 468     146     118      15
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     566     747 
   Size:        566     181
 
BbvCI CC'TCA_GC

Cuts at:      0     307     479     747 
   Size:        307     172     268

  Fragments arranged by size: 

                 307     268     172
 
BmgBI CAC'GTC

Cuts at:      0     606     747 
   Size:        606     141
 
Bpu10I CC'TnA_GC

Cuts at:      0     307     479     548     747 
   Size:        307     172      69     199

  Fragments arranged by size: 

                 307     199     172      69
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     747 
   Size:        328     419
 
BsaAI yAC'GTr

Cuts at:      0     489     747 
   Size:        489     258
 
BsaHI Gr'CG_yC

Cuts at:      0      43     747 
   Size:         43     704
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     403     523     526     541     544     580     595     598 
   Size:        403     120       3      15       3      36      15       3

Cuts at:    598     613     631     634     637     747 
   Size:         15      18       3       3     110

  Fragments arranged by size: 

                 403     120     110      36      18      15      15      15
                   3       3       3       3       3
 
BsiEI CG_ry'CG

Cuts at:      0      85     747 
   Size:         85     662
 
BstEII G'GTnAC_C

Cuts at:      0     372     747 
   Size:        372     375
 
EarI CTCTTCn'nnn_

Cuts at:      0     590     671     747 
   Size:        590      81      76

  Fragments arranged by size: 

                 590      81      76
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     743     747 
   Size:        743       4
 
EcoO109I rG'GnC_Cy

Cuts at:      0     234     462     495     747 
   Size:        234     228      33     252

  Fragments arranged by size: 

                 252     234     228      33
 
EcoRI G'AATT_C

Cuts at:      0     133     747 
   Size:        133     614
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     697     747 
   Size:        697      50
 
HindIII A'AGCT_T

Cuts at:      0     202     747 
   Size:        202     545
 
MfeI C'AATT_G

Cuts at:      0     363     747 
   Size:        363     384
 
PfoI T'CCnGG_A

Cuts at:      0      68     747 
   Size:         68     679
 
PshAI GACnn'nnGTC

Cuts at:      0      77     747 
   Size:         77     670
 
PsiI TTA'TAA

Cuts at:      0     641     747 
   Size:        641     106
 
SapI GCTCTTCn'nnn_

Cuts at:      0     671     747 
   Size:        671      76
 
SmlI C'TyrA_G

Cuts at:      0     138     196     343     747 
   Size:        138      58     147     404

  Fragments arranged by size: 

                 404     147     138      58
 
StuI AGG'CCT

Cuts at:      0     107     158     747 
   Size:        107      51     589

  Fragments arranged by size: 

                 589     107      51
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      71     747 
   Size:         71     676
 
TatI w'GTAC_w

Cuts at:      0     142     747 
   Size:        142     605
 

 Enzymes that do cut:

     AcuI    AflII     ApoI     BbsI    BbvCI    BmgBI   Bpu10I    BpuEI
    BsaAI    BsaHI    BseRI    BsiEI   BstEII     EarI  Eco57MI EcoO109I
    EcoRI     FalI  HindIII     MfeI     PfoI    PshAI     PsiI     SapI
     SmlI     StuI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI     BpmI     BsaI    BsaBI    BsaWI
    BsaXI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI    EcoRV     FseI     FspI    FspAI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI