Restriction map for
pF1KB7260
(Linear) (Six-base) MAPSORT of: KIBB7260.seq Check: 4374 from: 1 to: 936
KIBB7260.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AgeI A'CCGG_T
Cuts at: 0 362 936
Size: 362 574
AleI CACnn'nnGTG
Cuts at: 0 419 936
Size: 419 517
ApoI r'AATT_y
Cuts at: 0 518 936
Size: 518 418
BamHI G'GATC_C
Cuts at: 0 57 936
Size: 57 879
BanII G_rGCy'C
Cuts at: 0 900 936
Size: 900 36
BbvCI CC'TCA_GC
Cuts at: 0 913 936
Size: 913 23
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 210 244 936
Size: 210 34 692
Fragments arranged by size:
692 210 34
BclI T'GATC_A
Cuts at: 0 398 936
Size: 398 538
BfrBI ATG'CAT
Cuts at: 0 492 936
Size: 492 444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 936
Size: 538 398
Bpu10I CC'TnA_GC
Cuts at: 0 913 936
Size: 913 23
BsaI GGTCTCn'nnnn_
Cuts at: 0 731 936
Size: 731 205
BsaWI w'CCGG_w
Cuts at: 0 362 936
Size: 362 574
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 460 901 936
Size: 460 441 35
Fragments arranged by size:
460 441 35
BseYI C'CCAG_C
Cuts at: 0 117 615 936
Size: 117 498 321
Fragments arranged by size:
498 321 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 936
Size: 373 563
Bsp1286I G_dGCh'C
Cuts at: 0 900 936
Size: 900 36
BspHI T'CATG_A
Cuts at: 0 404 458 936
Size: 404 54 478
Fragments arranged by size:
478 404 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 936
Size: 134 802
BsrFI r'CCGG_y
Cuts at: 0 362 936
Size: 362 574
BsrGI T'GTAC_A
Cuts at: 0 830 936
Size: 830 106
BstEII G'GTnAC_C
Cuts at: 0 840 936
Size: 840 96
BstXI CCAn_nnnn'nTGG
Cuts at: 0 105 243 421 936
Size: 105 138 178 515
Fragments arranged by size:
515 178 138 105
BstYI r'GATC_y
Cuts at: 0 57 936
Size: 57 879
Bsu36I CC'TnA_GG
Cuts at: 0 874 936
Size: 874 62
BtgI C'CryG_G
Cuts at: 0 815 936
Size: 815 121
EaeI y'GGCC_r
Cuts at: 0 140 936
Size: 140 796
EarI CTCTTCn'nnn_
Cuts at: 0 804 936
Size: 804 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 936
Size: 538 398
EcoNI CCTnn'n_nnAGG
Cuts at: 0 295 936
Size: 295 641
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 477 509 936
Size: 477 32 427
Fragments arranged by size:
477 427 32
HincII GTy'rAC
Cuts at: 0 207 936
Size: 207 729
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 936
Size: 215 721
MscI TGG'CCA
Cuts at: 0 142 936
Size: 142 794
MslI CAynn'nnrTG
Cuts at: 0 172 419 847 936
Size: 172 247 428 89
Fragments arranged by size:
428 247 172 89
MspA1I CmG'CkG
Cuts at: 0 558 936
Size: 558 378
NsiI A_TGCA'T
Cuts at: 0 494 936
Size: 494 442
PasI CC'CwG_GG
Cuts at: 0 900 936
Size: 900 36
PvuII CAG'CTG
Cuts at: 0 558 936
Size: 558 378
SexAI A'CCwGG_T
Cuts at: 0 104 936
Size: 104 832
SfcI C'TryA_G
Cuts at: 0 67 867 936
Size: 67 800 69
Fragments arranged by size:
800 69 67
StyI C'CwwG_G
Cuts at: 0 633 936
Size: 633 303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 274 936
Size: 274 662
TatI w'GTAC_w
Cuts at: 0 176 830 936
Size: 176 654 106
Fragments arranged by size:
654 176 106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 279 936
Size: 279 657
XmnI GAAnn'nnTTC
Cuts at: 0 663 859 936
Size: 663 196 77
Fragments arranged by size:
663 196 77
Enzymes that do cut:
AgeI AleI ApoI BamHI BanII BbvCI BcgI BclI
BfrBI BpmI Bpu10I BsaI BsaWI BseRI BseYI BsgI
Bsp1286I BspHI BspMI BsrFI BsrGI BstEII BstXI BstYI
Bsu36I BtgI EaeI EarI Eco57MI EcoNI Hin4I HincII
MmeI MscI MslI MspA1I NsiI PasI PvuII SexAI
SfcI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BanI BbeI BbsI
BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpuEI BsaAI BsaBI BsaHI BsaXI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BsrBI BsrDI BssHII BssSI
BstAPI BstBI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI PacI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XhoI XmaI ZraI