(Linear) (Six-base) MAPSORT of: KIBB7258.seq  Check: 3628  from: 1  to: 933



KIBB7258.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

Acc65I G'GTAC_C

Cuts at:      0     604     933 
   Size:        604     329
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     933 
   Size:        200     733
 
ApoI r'AATT_y

Cuts at:      0      25     933 
   Size:         25     908
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     588     621     933 
   Size:        588      33     312

  Fragments arranged by size: 

                 588     312      33
 
BanI G'GyrC_C

Cuts at:      0     604     933 
   Size:        604     329
 
BanII G_rGCy'C

Cuts at:      0      55     933 
   Size:         55     878
 
BglII A'GATC_T

Cuts at:      0     326     933 
   Size:        326     607
 
BmgBI CAC'GTC

Cuts at:      0     437     933 
   Size:        437     496
 
Bpu10I CC'TnA_GC

Cuts at:      0     145     933 
   Size:        145     788
 
BsaBI GATnn'nnATC

Cuts at:      0     331     933 
   Size:        331     602
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     112     933 
   Size:        112     821
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     543     933 
   Size:        543     390
 
BsmI GAATG_Cn'

Cuts at:      0     885     933 
   Size:        885      48
 
Bsp1286I G_dGCh'C

Cuts at:      0      55     933 
   Size:         55     878
 
BspHI T'CATG_A

Cuts at:      0     398     933 
   Size:        398     535
 
BsrDI GCAATG_nn'

Cuts at:      0     608     722     890     933 
   Size:        608     114     168      43

  Fragments arranged by size: 

                 608     168     114      43
 
BsrGI T'GTAC_A

Cuts at:      0     764     933 
   Size:        764     169
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     615     933 
   Size:        615     318
 
BstYI r'GATC_y

Cuts at:      0     326     933 
   Size:        326     607
 
BtgI C'CryG_G

Cuts at:      0     728     933 
   Size:        728     205
 
DraIII CAC_nnn'GTG

Cuts at:      0     543     933 
   Size:        543     390
 
EarI CTCTTCn'nnn_

Cuts at:      0     219     933 
   Size:        219     714
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     933 
   Size:        200     733
 
EcoRI G'AATT_C

Cuts at:      0      25     933 
   Size:         25     908
 
HincII GTy'rAC

Cuts at:      0     243     933 
   Size:        243     690
 
KpnI G_GTAC'C

Cuts at:      0     608     933 
   Size:        608     325
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     510     933 
   Size:        510     423
 
MslI CAynn'nnrTG

Cuts at:      0     727     832     933 
   Size:        727     105     101

  Fragments arranged by size: 

                 727     105     101
 
NcoI C'CATG_G

Cuts at:      0     728     933 
   Size:        728     205
 
NspI r_CATG'y

Cuts at:      0     288     416     771     933 
   Size:        288     128     355     162

  Fragments arranged by size: 

                 355     288     162     128
 
SfcI C'TryA_G

Cuts at:      0     645     933 
   Size:        645     288
 
StyI C'CwwG_G

Cuts at:      0     728     933 
   Size:        728     205
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     180     933 
   Size:        180     753
 
TatI w'GTAC_w

Cuts at:      0     303     754     764     933 
   Size:        303     451      10     169

  Fragments arranged by size: 

                 451     303     169      10
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     273     468     933 
   Size:        273     195     465

  Fragments arranged by size: 

                 465     273     195
 

 Enzymes that do cut:

   Acc65I     AcuI     ApoI     BaeI     BanI    BanII    BglII    BmgBI
   Bpu10I    BsaBI    BseRI     BsgI     BsmI Bsp1286I    BspHI    BsrDI
    BsrGI    BstXI    BstYI     BtgI   DraIII     EarI  Eco57MI    EcoRI
   HincII     KpnI     MmeI     MslI     NcoI     NspI     SfcI     StyI
    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspMI
    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     MfeI
     MluI     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI