Restriction map for
pF1KB7256
(Linear) (Six-base) MAPSORT of: KIBB7256.seq Check: 3931 from: 1 to: 1323
KIBB7256.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AccI GT'mk_AC
Cuts at: 0 176 548 1323
Size: 176 372 775
Fragments arranged by size:
775 372 176
Acc65I G'GTAC_C
Cuts at: 0 248 1323
Size: 248 1075
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 834 1153 1323
Size: 834 319 170
Fragments arranged by size:
834 319 170
AfeI AGC'GCT
Cuts at: 0 666 1323
Size: 666 657
AflIII A'CryG_T
Cuts at: 0 860 1323
Size: 860 463
ApaI G_GGCC'C
Cuts at: 0 345 1071 1323
Size: 345 726 252
Fragments arranged by size:
726 345 252
ApoI r'AATT_y
Cuts at: 0 53 271 973 1323
Size: 53 218 702 350
Fragments arranged by size:
702 350 218 53
BanI G'GyrC_C
Cuts at: 0 248 710 873 1323
Size: 248 462 163 450
Fragments arranged by size:
462 450 248 163
BanII G_rGCy'C
Cuts at: 0 345 571 1071 1323
Size: 345 226 500 252
Fragments arranged by size:
500 345 252 226
BbsI GAAGACnn'nnnn_
Cuts at: 0 548 581 840 1323
Size: 548 33 259 483
Fragments arranged by size:
548 483 259 33
BglI GCCn_nnn'nGGC
Cuts at: 0 340 1323
Size: 340 983
BglII A'GATC_T
Cuts at: 0 314 1323
Size: 314 1009
BlpI GC'TnA_GC
Cuts at: 0 948 1323
Size: 948 375
Bme1580I G_kGCm'C
Cuts at: 0 345 911 1071 1308 1323
Size: 345 566 160 237 15
Fragments arranged by size:
566 345 237 160 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 914 1323
Size: 914 409
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 689 1323
Size: 273 416 634
Fragments arranged by size:
634 416 273
BsaI GGTCTCn'nnnn_
Cuts at: 0 135 459 1323
Size: 135 324 864
Fragments arranged by size:
864 324 135
BsaBI GATnn'nnATC
Cuts at: 0 71 1323
Size: 71 1252
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1195 1225 1323
Size: 1195 30 98
Fragments arranged by size:
1195 98 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 579 1323
Size: 579 744
BseYI C'CCAG_C
Cuts at: 0 1118 1269 1323
Size: 1118 151 54
Fragments arranged by size:
1118 151 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 1323
Size: 483 840
BsiHKAI G_wGCw'C
Cuts at: 0 895 1112 1297 1323
Size: 895 217 185 26
Fragments arranged by size:
895 217 185 26
BsmI GAATG_Cn'
Cuts at: 0 524 1323
Size: 524 799
Bsp1286I G_dGCh'C
Cuts at: 0 345 571 895 911 1071 1112 1297 1308
Size: 345 226 324 16 160 41 185 11
Cuts at: 1308 1323
Size: 15
Fragments arranged by size:
345 324 226 185 160 41 16 15
11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 794 1323
Size: 794 529
BstXI CCAn_nnnn'nTGG
Cuts at: 0 450 831 927 966 1323
Size: 450 381 96 39 357
Fragments arranged by size:
450 381 357 96 39
BstYI r'GATC_y
Cuts at: 0 314 1274 1323
Size: 314 960 49
Fragments arranged by size:
960 314 49
BtgI C'CryG_G
Cuts at: 0 877 1323
Size: 877 446
DraIII CAC_nnn'GTG
Cuts at: 0 883 1292 1323
Size: 883 409 31
Fragments arranged by size:
883 409 31
DrdI GACnn_nn'nnGTC
Cuts at: 0 190 1323
Size: 190 1133
EarI CTCTTCn'nnn_
Cuts at: 0 1252 1323
Size: 1252 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 689 834 1153 1323
Size: 273 416 145 319 170
Fragments arranged by size:
416 319 273 170 145
EcoNI CCTnn'n_nnAGG
Cuts at: 0 561 1323
Size: 561 762
EcoO109I rG'GnC_Cy
Cuts at: 0 342 1067 1323
Size: 342 725 256
Fragments arranged by size:
725 342 256
EcoRI G'AATT_C
Cuts at: 0 53 1323
Size: 53 1270
EcoRV GAT'ATC
Cuts at: 0 351 1323
Size: 351 972
FspI TGC'GCA
Cuts at: 0 706 1323
Size: 706 617
HaeII r_GCGC'y
Cuts at: 0 668 1323
Size: 668 655
HincII GTy'rAC
Cuts at: 0 858 1323
Size: 858 465
KpnI G_GTAC'C
Cuts at: 0 252 1323
Size: 252 1071
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 890 1323
Size: 890 433
MslI CAynn'nnrTG
Cuts at: 0 40 448 763 925 1323
Size: 40 408 315 162 398
Fragments arranged by size:
408 398 315 162 40
NdeI CA'TA_TG
Cuts at: 0 477 1323
Size: 477 846
NspI r_CATG'y
Cuts at: 0 297 864 1323
Size: 297 567 459
Fragments arranged by size:
567 459 297
PasI CC'CwG_GG
Cuts at: 0 1064 1323
Size: 1064 259
PciI A'CATG_T
Cuts at: 0 860 1323
Size: 860 463
PfoI T'CCnGG_A
Cuts at: 0 280 1323
Size: 280 1043
PspOMI G'GGCC_C
Cuts at: 0 341 1067 1323
Size: 341 726 256
Fragments arranged by size:
726 341 256
PstI C_TGCA'G
Cuts at: 0 51 659 1323
Size: 51 608 664
Fragments arranged by size:
664 608 51
SapI GCTCTTCn'nnn_
Cuts at: 0 1252 1323
Size: 1252 71
SexAI A'CCwGG_T
Cuts at: 0 869 1323
Size: 869 454
SfcI C'TryA_G
Cuts at: 0 47 655 1323
Size: 47 608 668
Fragments arranged by size:
668 608 47
SspI AAT'ATT
Cuts at: 0 230 1323
Size: 230 1093
StuI AGG'CCT
Cuts at: 0 1050 1323
Size: 1050 273
StyI C'CwwG_G
Cuts at: 0 161 1323
Size: 161 1162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 807 1253 1323
Size: 807 446 70
Fragments arranged by size:
807 446 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1195 1227 1323
Size: 1195 32 96
Fragments arranged by size:
1195 96 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 177 831 1323
Size: 177 654 492
Fragments arranged by size:
654 492 177
XmnI GAAnn'nnTTC
Cuts at: 0 578 1323
Size: 578 745
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII ApaI ApoI BanI
BanII BbsI BglI BglII BlpI Bme1580I BplI BpmI
BsaI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspMI BstXI BstYI BtgI DraIII DrdI EarI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII HincII
KpnI MmeI MslI NdeI NspI PasI PciI PfoI
PspOMI PstI SapI SexAI SfcI SspI StuI StyI
TaqII TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AhdI AleI AlfI
AloI AlwNI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BmgBI BmrI BmtI Bpu10I BpuEI BsaAI BsaHI BsaWI
BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI EaeI EagI EciI
EcoICRI FalI FseI FspAI Hin4I HindIII HpaI KasI
MfeI MluI MscI MspA1I NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SwaI TatI Tth111I XbaI XhoI XmaI ZraI