Restriction map for
pF1KB7255
(Linear) (Six-base) MAPSORT of: KIBB7255.seq Check: 6098 from: 1 to: 933
KIBB7255.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 727 933
Size: 727 206
AccI GT'mk_AC
Cuts at: 0 206 933
Size: 206 727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 922 933
Size: 697 225 11
Fragments arranged by size:
697 225 11
AleI CACnn'nnGTG
Cuts at: 0 22 838 933
Size: 22 816 95
Fragments arranged by size:
816 95 22
ApoI r'AATT_y
Cuts at: 0 98 933
Size: 98 835
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 50 83 933
Size: 50 33 850
Fragments arranged by size:
850 50 33
BanII G_rGCy'C
Cuts at: 0 900 933
Size: 900 33
BciVI GTATCCnnnnn_n'
Cuts at: 0 51 933
Size: 51 882
BclI T'GATC_A
Cuts at: 0 143 933
Size: 143 790
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 933
Size: 434 499
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 210 933
Size: 210 723
BseYI C'CCAG_C
Cuts at: 0 296 933
Size: 296 637
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 705 933
Size: 705 228
BsiEI CG_ry'CG
Cuts at: 0 329 933
Size: 329 604
BsmI GAATG_Cn'
Cuts at: 0 468 933
Size: 468 465
Bsp1286I G_dGCh'C
Cuts at: 0 900 933
Size: 900 33
BspHI T'CATG_A
Cuts at: 0 245 933
Size: 245 688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 409 727 933
Size: 409 318 206
Fragments arranged by size:
409 318 206
BsrGI T'GTAC_A
Cuts at: 0 176 933
Size: 176 757
BstYI r'GATC_y
Cuts at: 0 610 933
Size: 610 323
Bsu36I CC'TnA_GG
Cuts at: 0 256 874 933
Size: 256 618 59
Fragments arranged by size:
618 256 59
BtgI C'CryG_G
Cuts at: 0 350 836 933
Size: 350 486 97
Fragments arranged by size:
486 350 97
BtsI GCAGTG_nn'
Cuts at: 0 734 933
Size: 734 199
DraIII CAC_nnn'GTG
Cuts at: 0 632 933
Size: 632 301
EaeI y'GGCC_r
Cuts at: 0 326 371 623 933
Size: 326 45 252 310
Fragments arranged by size:
326 310 252 45
EagI C'GGCC_G
Cuts at: 0 326 933
Size: 326 607
EarI CTCTTCn'nnn_
Cuts at: 0 609 898 933
Size: 609 289 35
Fragments arranged by size:
609 289 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 697 922 933
Size: 434 263 225 11
Fragments arranged by size:
434 263 225 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 407 439 933
Size: 407 32 494
Fragments arranged by size:
494 407 32
HindIII A'AGCT_T
Cuts at: 0 556 933
Size: 556 377
MscI TGG'CCA
Cuts at: 0 373 625 933
Size: 373 252 308
Fragments arranged by size:
373 308 252
MslI CAynn'nnrTG
Cuts at: 0 22 115 244 430 775 838 933
Size: 22 93 129 186 345 63 95
Fragments arranged by size:
345 186 129 95 93 63 22
NcoI C'CATG_G
Cuts at: 0 350 933
Size: 350 583
NspI r_CATG'y
Cuts at: 0 408 441 933
Size: 408 33 492
Fragments arranged by size:
492 408 33
PstI C_TGCA'G
Cuts at: 0 741 933
Size: 741 192
SfcI C'TryA_G
Cuts at: 0 62 308 737 867 933
Size: 62 246 429 130 66
Fragments arranged by size:
429 246 130 66 62
SspI AAT'ATT
Cuts at: 0 926 933
Size: 926 7
StuI AGG'CCT
Cuts at: 0 709 933
Size: 709 224
StyI C'CwwG_G
Cuts at: 0 350 692 933
Size: 350 342 241
Fragments arranged by size:
350 342 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 186 439 933
Size: 186 253 494
Fragments arranged by size:
494 253 186
TatI w'GTAC_w
Cuts at: 0 176 933
Size: 176 757
XmnI GAAnn'nnTTC
Cuts at: 0 590 933
Size: 590 343
Enzymes that do cut:
AarI AccI AcuI AleI ApoI BaeI BanII BciVI
BclI BpmI BseRI BseYI BsgI BsiEI BsmI Bsp1286I
BspHI BspMI BsrGI BstYI Bsu36I BtgI BtsI DraIII
EaeI EagI EarI Eco57MI Hin4I HindIII MscI MslI
NcoI NspI PstI SfcI SspI StuI StyI TaqII
TatI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BamHI BanI BbeI BbsI BbvCI BcgI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BsiHKAI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI
ClaI CspCI DraI DrdI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI