Restriction map for
pF1KB7252
(Linear) (Six-base) MAPSORT of: KIBB7252.seq Check: 9854 from: 1 to: 1443
KIBB7252.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1223 1314 1443
Size: 1223 91 129
Fragments arranged by size:
1223 129 91
AleI CACnn'nnGTG
Cuts at: 0 629 1443
Size: 629 814
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 470 502 773 805 1443
Size: 470 32 271 32 638
Fragments arranged by size:
638 470 271 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 109 537 720 1443
Size: 109 428 183 723
Fragments arranged by size:
723 428 183 109
ApaI G_GGCC'C
Cuts at: 0 49 74 553 697 853 997 1443
Size: 49 25 479 144 156 144 446
Fragments arranged by size:
479 446 156 144 144 49 25
ApaLI G'TGCA_C
Cuts at: 0 893 1443
Size: 893 550
ApoI r'AATT_y
Cuts at: 0 1138 1443
Size: 1138 305
AvaI C'yCGr_G
Cuts at: 0 611 1381 1443
Size: 611 770 62
Fragments arranged by size:
770 611 62
BanII G_rGCy'C
Cuts at: 0 49 74 130 203 326 553 697 827
Size: 49 25 56 73 123 227 144 130
Cuts at: 827 853 862 997 1122 1238 1443
Size: 26 9 135 125 116 205
Fragments arranged by size:
227 205 144 135 130 125 123 116
73 56 49 26 25 9
BbvCI CC'TCA_GC
Cuts at: 0 1161 1443
Size: 1161 282
BclI T'GATC_A
Cuts at: 0 113 1443
Size: 113 1330
BglII A'GATC_T
Cuts at: 0 959 1443
Size: 959 484
BlpI GC'TnA_GC
Cuts at: 0 98 1403 1443
Size: 98 1305 40
Fragments arranged by size:
1305 98 40
Bme1580I G_kGCm'C
Cuts at: 0 49 74 553 697 853 897 997 1443
Size: 49 25 479 144 156 44 100 446
Fragments arranged by size:
479 446 156 144 100 49 44 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 623 1223 1443
Size: 623 600 220
Fragments arranged by size:
623 600 220
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 123 1443
Size: 123 1320
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 358 661 712 778 1257 1286 1443
Size: 178 180 303 51 66 479 29 157
Fragments arranged by size:
479 303 180 178 157 66 51 29
Bpu10I CC'TnA_GC
Cuts at: 0 1161 1443
Size: 1161 282
BsaI GGTCTCn'nnnn_
Cuts at: 0 1257 1443
Size: 1257 186
BsaHI Gr'CG_yC
Cuts at: 0 4 1443
Size: 4 1439
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 470 497 500 527 889 892 919 922
Size: 470 27 3 27 362 3 27 3
Cuts at: 922 958 988 1004 1034 1443
Size: 36 30 16 30 409
Fragments arranged by size:
470 409 362 36 30 30 27 27
27 16 3 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 897 1443
Size: 897 546
BseYI C'CCAG_C
Cuts at: 0 119 325 388 562 820 925 1443
Size: 119 206 63 174 258 105 518
Fragments arranged by size:
518 258 206 174 119 105 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 1443
Size: 82 1361
BsiHKAI G_wGCw'C
Cuts at: 0 897 1122 1443
Size: 897 225 321
Fragments arranged by size:
897 321 225
BsmBI CGTCTCn'nnnn_
Cuts at: 0 978 1443
Size: 978 465
Bsp1286I G_dGCh'C
Cuts at: 0 49 74 130 203 326 553 697 827
Size: 49 25 56 73 123 227 144 130
Cuts at: 827 853 862 897 997 1122 1238 1443
Size: 26 9 35 100 125 116 205
Fragments arranged by size:
227 205 144 130 125 123 116 100
73 56 49 35 26 25 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1223 1314 1443
Size: 1223 91 129
Fragments arranged by size:
1223 129 91
BstXI CCAn_nnnn'nTGG
Cuts at: 0 396 802 1443
Size: 396 406 641
Fragments arranged by size:
641 406 396
BstYI r'GATC_y
Cuts at: 0 959 1443
Size: 959 484
Bsu36I CC'TnA_GG
Cuts at: 0 405 654 1008 1443
Size: 405 249 354 435
Fragments arranged by size:
435 405 354 249
BtgI C'CryG_G
Cuts at: 0 867 1443
Size: 867 576
BtsI GCAGTG_nn'
Cuts at: 0 1093 1443
Size: 1093 350
EaeI y'GGCC_r
Cuts at: 0 804 1443
Size: 804 639
EcoICRI GAG'CTC
Cuts at: 0 1120 1443
Size: 1120 323
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 358 661 712 778 1257 1286 1443
Size: 178 180 303 51 66 479 29 157
Fragments arranged by size:
479 303 180 178 157 66 51 29
EcoO109I rG'GnC_Cy
Cuts at: 0 46 53 70 71 534 550 693 694
Size: 46 7 17 1 463 16 143 1
Cuts at: 694 850 994 1175 1443
Size: 156 144 181 268
Fragments arranged by size:
463 268 181 156 144 143 46 17
16 7 1 1
EcoRI G'AATT_C
Cuts at: 0 1138 1443
Size: 1138 305
EcoRV GAT'ATC
Cuts at: 0 448 1443
Size: 448 995
HaeII r_GCGC'y
Cuts at: 0 801 1443
Size: 801 642
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 236 268 958 990 1443
Size: 236 32 690 32 453
Fragments arranged by size:
690 453 236 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1229 1443
Size: 1229 214
MscI TGG'CCA
Cuts at: 0 806 1443
Size: 806 637
MslI CAynn'nnrTG
Cuts at: 0 629 946 1348 1354 1443
Size: 629 317 402 6 89
Fragments arranged by size:
629 402 317 89 6
MspA1I CmG'CkG
Cuts at: 0 119 290 1443
Size: 119 171 1153
Fragments arranged by size:
1153 171 119
NspI r_CATG'y
Cuts at: 0 587 1443
Size: 587 856
PasI CC'CwG_GG
Cuts at: 0 709 846 1443
Size: 709 137 597
Fragments arranged by size:
709 597 137
PflMI CCAn_nnn'nTGG
Cuts at: 0 92 1443
Size: 92 1351
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 470 502 1443
Size: 470 32 941
Fragments arranged by size:
941 470 32
PpuMI rG'GwC_Cy
Cuts at: 0 53 1175 1443
Size: 53 1122 268
Fragments arranged by size:
1122 268 53
PspOMI G'GGCC_C
Cuts at: 0 45 70 549 693 849 993 1443
Size: 45 25 479 144 156 144 450
Fragments arranged by size:
479 450 156 144 144 45 25
PstI C_TGCA'G
Cuts at: 0 312 692 1443
Size: 312 380 751
Fragments arranged by size:
751 380 312
PvuII CAG'CTG
Cuts at: 0 119 1443
Size: 119 1324
SacI G_AGCT'C
Cuts at: 0 1122 1443
Size: 1122 321
SbfI CC_TGCA'GG
Cuts at: 0 692 1443
Size: 692 751
SfcI C'TryA_G
Cuts at: 0 308 688 1179 1443
Size: 308 380 491 264
Fragments arranged by size:
491 380 308 264
SmaI CCC'GGG
Cuts at: 0 613 1443
Size: 613 830
StyI C'CwwG_G
Cuts at: 0 30 203 1319 1443
Size: 30 173 1116 124
Fragments arranged by size:
1116 173 124 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1299 1443
Size: 1299 144
TatI w'GTAC_w
Cuts at: 0 878 1443
Size: 878 565
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 497 529 889 921 1004 1036 1443
Size: 497 32 360 32 83 32 407
Fragments arranged by size:
497 407 360 83 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 970 1443
Size: 970 473
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 333 396 636 843 1236 1443
Size: 333 63 240 207 393 207
Fragments arranged by size:
393 333 240 207 207 63
XmaI C'CCGG_G
Cuts at: 0 611 1443
Size: 611 832
Enzymes that do cut:
AarI AleI AloI AlwNI ApaI ApaLI ApoI AvaI
BanII BbvCI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspMI BstXI BstYI Bsu36I BtgI
BtsI EaeI EcoICRI Eco57MI EcoO109I EcoRI EcoRV HaeII
Hin4I MmeI MscI MslI MspA1I NspI PasI PflMI
PpiI PpuMI PspOMI PstI PvuII SacI SbfI SfcI
SmaI StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbsI BcgI BciVI BfrBI BglI
BmgBI BmtI BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI
BsmI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoNI FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PfoI PmeI PmlI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
XbaI XhoI XmnI ZraI