Restriction map for
pF1KB7250
(Linear) (Six-base) MAPSORT of: KIBB7250.seq Check: 2704 from: 1 to: 444
KIBB7250.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 349 444
Size: 349 95
AhdI GACnn_n'nnGTC
Cuts at: 0 172 444
Size: 172 272
AlwNI CAG_nnn'CTG
Cuts at: 0 315 412 444
Size: 315 97 32
Fragments arranged by size:
315 97 32
ApaI G_GGCC'C
Cuts at: 0 195 274 292 444
Size: 195 79 18 152
Fragments arranged by size:
195 152 79 18
AvaI C'yCGr_G
Cuts at: 0 267 285 444
Size: 267 18 159
Fragments arranged by size:
267 159 18
BamHI G'GATC_C
Cuts at: 0 8 444
Size: 8 436
BanI G'GyrC_C
Cuts at: 0 152 211 337 444
Size: 152 59 126 107
Fragments arranged by size:
152 126 107 59
BanII G_rGCy'C
Cuts at: 0 116 130 195 274 292 444
Size: 116 14 65 79 18 152
Fragments arranged by size:
152 116 79 65 18 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 285 444
Size: 285 159
BglI GCCn_nnn'nGGC
Cuts at: 0 199 220 444
Size: 199 21 224
Fragments arranged by size:
224 199 21
Bme1580I G_kGCm'C
Cuts at: 0 195 274 292 340 444
Size: 195 79 18 48 104
Fragments arranged by size:
195 104 79 48 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 444
Size: 181 263
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 444
Size: 31 413
BseYI C'CCAG_C
Cuts at: 0 117 308 333 421 444
Size: 117 191 25 88 23
Fragments arranged by size:
191 117 88 25 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 444
Size: 370 74
BsiEI CG_ry'CG
Cuts at: 0 20 392 396 444
Size: 20 372 4 48
Fragments arranged by size:
372 48 20 4
Bsp1286I G_dGCh'C
Cuts at: 0 116 130 195 274 292 340 444
Size: 116 14 65 79 18 48 104
Fragments arranged by size:
116 104 79 65 48 18 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 349 444
Size: 349 95
BsrFI r'CCGG_y
Cuts at: 0 214 387 391 395 444
Size: 214 173 4 4 49
Fragments arranged by size:
214 173 49 4 4
BstXI CCAn_nnnn'nTGG
Cuts at: 0 439 444
Size: 439 5
BstYI r'GATC_y
Cuts at: 0 8 444
Size: 8 436
EaeI y'GGCC_r
Cuts at: 0 17 255 389 393 444
Size: 17 238 134 4 51
Fragments arranged by size:
238 134 51 17 4
EagI C'GGCC_G
Cuts at: 0 17 389 393 444
Size: 17 372 4 51
Fragments arranged by size:
372 51 17 4
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 444
Size: 181 263
EcoO109I rG'GnC_Cy
Cuts at: 0 87 192 271 289 299 444
Size: 87 105 79 18 10 145
Fragments arranged by size:
145 105 87 79 18 10
FseI GG_CCGG'CC
Cuts at: 0 395 444
Size: 395 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 158 190 444
Size: 158 32 254
Fragments arranged by size:
254 158 32
HindIII A'AGCT_T
Cuts at: 0 181 444
Size: 181 263
MslI CAynn'nnrTG
Cuts at: 0 437 444
Size: 437 7
NaeI GCC'GGC
Cuts at: 0 216 393 397 444
Size: 216 177 4 47
Fragments arranged by size:
216 177 47 4
NgoMIV G'CCGG_C
Cuts at: 0 214 391 395 444
Size: 214 177 4 49
Fragments arranged by size:
214 177 49 4
PasI CC'CwG_GG
Cuts at: 0 24 107 444
Size: 24 83 337
Fragments arranged by size:
337 83 24
PfoI T'CCnGG_A
Cuts at: 0 245 444
Size: 245 199
PpuMI rG'GwC_Cy
Cuts at: 0 87 299 444
Size: 87 212 145
Fragments arranged by size:
212 145 87
PspOMI G'GGCC_C
Cuts at: 0 191 270 288 444
Size: 191 79 18 156
Fragments arranged by size:
191 156 79 18
PstI C_TGCA'G
Cuts at: 0 53 146 444
Size: 53 93 298
Fragments arranged by size:
298 93 53
SanDI GG'GwC_CC
Cuts at: 0 87 444
Size: 87 357
SfcI C'TryA_G
Cuts at: 0 49 142 444
Size: 49 93 302
Fragments arranged by size:
302 93 49
Enzymes that do cut:
AarI AhdI AlwNI ApaI AvaI BamHI BanI BanII
BbsI BglI Bme1580I BpmI BseRI BseYI BsgI BsiEI
Bsp1286I BspMI BsrFI BstXI BstYI EaeI EagI Eco57MI
EcoO109I FseI Hin4I HindIII MslI NaeI NgoMIV PasI
PfoI PpuMI PspOMI PstI SanDI SfcI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MspA1I NarI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI