(Linear) (Six-base) MAPSORT of: KIBB7249.seq  Check: 8851  from: 1  to: 369



KIBB7249.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     266     369 
   Size:        137     129     103

  Fragments arranged by size: 

                 137     129     103
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      54     151     369 
   Size:         54      97     218

  Fragments arranged by size: 

                 218      97      54
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     330     369 
   Size:        330      39
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     369 
   Size:         31     338
 
BstYI r'GATC_y

Cuts at:      0     192     369 
   Size:        192     177
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     137     266     369 
   Size:         31     106     129     103

  Fragments arranged by size: 

                 129     106     103      31
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      61     369 
   Size:         61     308
 
EcoRV GAT'ATC

Cuts at:      0     222     369 
   Size:        222     147
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     288     369 
   Size:        288      81
 
HindIII A'AGCT_T

Cuts at:      0     230     369 
   Size:        230     139
 
MspA1I CmG'CkG

Cuts at:      0     351     369 
   Size:        351      18
 
PfoI T'CCnGG_A

Cuts at:      0       8     369 
   Size:          8     361
 
PstI C_TGCA'G

Cuts at:      0     360     369 
   Size:        360       9
 
PvuII CAG'CTG

Cuts at:      0     351     369 
   Size:        351      18
 
ScaI AGT'ACT

Cuts at:      0     161     369 
   Size:        161     208
 
SfcI C'TryA_G

Cuts at:      0     356     369 
   Size:        356      13
 
StyI C'CwwG_G

Cuts at:      0      62     369 
   Size:         62     307
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      26     369 
   Size:         26     343
 
TatI w'GTAC_w

Cuts at:      0     159     369 
   Size:        159     210
 
XmnI GAAnn'nnTTC

Cuts at:      0     268     369 
   Size:        268     101
 

 Enzymes that do cut:

     AcuI     BmrI     BplI     BpmI    BstYI  Eco57MI    EcoNI    EcoRV
     FalI  HindIII   MspA1I     PfoI     PstI    PvuII     ScaI     SfcI
     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI EcoO109I    EcoRI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI