Restriction map for
pF1KB7242
(Linear) (Six-base) MAPSORT of: KIBB7242.seq Check: 3099 from: 1 to: 552
KIBB7242.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 552
Size: 271 281
AflIII A'CryG_T
Cuts at: 0 326 552
Size: 326 226
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 186 552
Size: 186 366
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 313 345 552
Size: 313 32 207
Fragments arranged by size:
313 207 32
AlwNI CAG_nnn'CTG
Cuts at: 0 318 552
Size: 318 234
ApoI r'AATT_y
Cuts at: 0 524 552
Size: 524 28
BanII G_rGCy'C
Cuts at: 0 509 552
Size: 509 43
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 221 255 552
Size: 221 34 297
Fragments arranged by size:
297 221 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 38 52 552
Size: 38 14 500
Fragments arranged by size:
500 38 14
BclI T'GATC_A
Cuts at: 0 377 552
Size: 377 175
BmgBI CAC'GTC
Cuts at: 0 387 552
Size: 387 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 388 506 552
Size: 318 70 118 46
Fragments arranged by size:
318 118 70 46
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 316 346 552
Size: 316 30 206
Fragments arranged by size:
316 206 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 266 552
Size: 266 286
BseYI C'CCAG_C
Cuts at: 0 502 552
Size: 502 50
BsiHKAI G_wGCw'C
Cuts at: 0 227 552
Size: 227 325
Bsp1286I G_dGCh'C
Cuts at: 0 227 509 552
Size: 227 282 43
Fragments arranged by size:
282 227 43
BsrDI GCAATG_nn'
Cuts at: 0 108 209 552
Size: 108 101 343
Fragments arranged by size:
343 108 101
BstEII G'GTnAC_C
Cuts at: 0 407 552
Size: 407 145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 78 113 171 206 552
Size: 78 35 58 35 346
Fragments arranged by size:
346 78 58 35 35
EarI CTCTTCn'nnn_
Cuts at: 0 180 228 552
Size: 180 48 324
Fragments arranged by size:
324 180 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 318 388 506 552
Size: 271 47 70 118 46
Fragments arranged by size:
271 118 70 47 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 36 68 246 278 552
Size: 36 32 178 32 274
Fragments arranged by size:
274 178 36 32 32
MfeI C'AATT_G
Cuts at: 0 122 552
Size: 122 430
NspI r_CATG'y
Cuts at: 0 330 552
Size: 330 222
PciI A'CATG_T
Cuts at: 0 326 552
Size: 326 226
SapI GCTCTTCn'nnn_
Cuts at: 0 180 228 552
Size: 180 48 324
Fragments arranged by size:
324 180 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 426 552
Size: 426 126
TatI w'GTAC_w
Cuts at: 0 356 552
Size: 356 196
Enzymes that do cut:
AcuI AflIII AlfI AloI AlwNI ApoI BanII BcgI
BciVI BclI BmgBI BpmI BsaXI BseRI BseYI BsiHKAI
Bsp1286I BsrDI BstEII CspCI EarI Eco57MI Hin4I MfeI
NspI PciI SapI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BfrBI
BglI BglII BlpI Bme1580I BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI