Restriction map for
pF1KB7240
(Linear) (Six-base) MAPSORT of: KIBB7240.seq Check: 483 from: 1 to: 933
KIBB7240.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 170 933
Size: 170 763
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 922 933
Size: 696 226 11
Fragments arranged by size:
696 226 11
AflIII A'CryG_T
Cuts at: 0 338 731 933
Size: 338 393 202
Fragments arranged by size:
393 338 202
AleI CACnn'nnGTG
Cuts at: 0 115 933
Size: 115 818
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 259 291 933
Size: 259 32 642
Fragments arranged by size:
642 259 32
AlwNI CAG_nnn'CTG
Cuts at: 0 28 933
Size: 28 905
ApoI r'AATT_y
Cuts at: 0 508 933
Size: 508 425
BanII G_rGCy'C
Cuts at: 0 50 68 933
Size: 50 18 865
Fragments arranged by size:
865 50 18
BbvCI CC'TCA_GC
Cuts at: 0 187 933
Size: 187 746
BciVI GTATCCnnnnn_n'
Cuts at: 0 828 933
Size: 828 105
BfrBI ATG'CAT
Cuts at: 0 290 933
Size: 290 643
Bme1580I G_kGCm'C
Cuts at: 0 848 933
Size: 848 85
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 933
Size: 52 881
Bpu10I CC'TnA_GC
Cuts at: 0 187 933
Size: 187 746
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 259 289 670 700 933
Size: 259 30 381 30 233
Fragments arranged by size:
381 259 233 30 30
BseYI C'CCAG_C
Cuts at: 0 43 628 933
Size: 43 585 305
Fragments arranged by size:
585 305 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 933
Size: 148 785
BsiHKAI G_wGCw'C
Cuts at: 0 68 933
Size: 68 865
BsmI GAATG_Cn'
Cuts at: 0 292 766 933
Size: 292 474 167
Fragments arranged by size:
474 292 167
Bsp1286I G_dGCh'C
Cuts at: 0 50 68 848 933
Size: 50 18 780 85
Fragments arranged by size:
780 85 50 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 170 689 933
Size: 170 519 244
Fragments arranged by size:
519 244 170
BsrDI GCAATG_nn'
Cuts at: 0 356 933
Size: 356 577
BsrGI T'GTAC_A
Cuts at: 0 392 933
Size: 392 541
BstYI r'GATC_y
Cuts at: 0 757 933
Size: 757 176
Bsu36I CC'TnA_GG
Cuts at: 0 874 933
Size: 874 59
BtgI C'CryG_G
Cuts at: 0 800 933
Size: 800 133
EaeI y'GGCC_r
Cuts at: 0 371 933
Size: 371 562
EarI CTCTTCn'nnn_
Cuts at: 0 85 933
Size: 85 848
EcoICRI GAG'CTC
Cuts at: 0 66 933
Size: 66 867
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 696 922 933
Size: 52 644 226 11
Fragments arranged by size:
644 226 52 11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 438 933
Size: 438 495
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 685 717 933
Size: 685 32 216
Fragments arranged by size:
685 216 32
HincII GTy'rAC
Cuts at: 0 582 933
Size: 582 351
MscI TGG'CCA
Cuts at: 0 373 933
Size: 373 560
MslI CAynn'nnrTG
Cuts at: 0 115 172 933
Size: 115 57 761
Fragments arranged by size:
761 115 57
MspA1I CmG'CkG
Cuts at: 0 72 933
Size: 72 861
NcoI C'CATG_G
Cuts at: 0 800 933
Size: 800 133
NsiI A_TGCA'T
Cuts at: 0 292 933
Size: 292 641
NspI r_CATG'y
Cuts at: 0 342 735 933
Size: 342 393 198
Fragments arranged by size:
393 342 198
PciI A'CATG_T
Cuts at: 0 338 731 933
Size: 338 393 202
Fragments arranged by size:
393 338 202
PflMI CCAn_nnn'nTGG
Cuts at: 0 735 933
Size: 735 198
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 933
Size: 259 32 642
Fragments arranged by size:
642 259 32
PstI C_TGCA'G
Cuts at: 0 765 933
Size: 765 168
PvuII CAG'CTG
Cuts at: 0 72 933
Size: 72 861
SacI G_AGCT'C
Cuts at: 0 68 933
Size: 68 865
SfcI C'TryA_G
Cuts at: 0 378 761 913 933
Size: 378 383 152 20
Fragments arranged by size:
383 378 152 20
StuI AGG'CCT
Cuts at: 0 455 933
Size: 455 478
StyI C'CwwG_G
Cuts at: 0 800 933
Size: 800 133
TatI w'GTAC_w
Cuts at: 0 176 392 773 933
Size: 176 216 381 160
Fragments arranged by size:
381 216 176 160
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 89 121 670 702 933
Size: 89 32 549 32 231
Fragments arranged by size:
549 231 89 32 32
Enzymes that do cut:
AarI AcuI AflIII AleI AloI AlwNI ApoI BanII
BbvCI BciVI BfrBI Bme1580I BpmI Bpu10I BsaXI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrGI BstYI
Bsu36I BtgI EaeI EarI EcoICRI Eco57MI FalI Hin4I
HincII MscI MslI MspA1I NcoI NsiI NspI PciI
PflMI PpiI PstI PvuII SacI SfcI StuI StyI
TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbsI BcgI BclI BglI
BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoNI EcoO109I EcoRI EcoRV
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PasI PfoI PmeI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI