Restriction map for
pF1KB7239
(Linear) (Six-base) MAPSORT of: KIBB7239.seq Check: 5135 from: 1 to: 672
KIBB7239.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 145 253 672
Size: 145 108 419
Fragments arranged by size:
419 145 108
AccI GT'mk_AC
Cuts at: 0 70 177 672
Size: 70 107 495
Fragments arranged by size:
495 107 70
Acc65I G'GTAC_C
Cuts at: 0 98 672
Size: 98 574
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 672
Size: 400 272
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 132 672
Size: 132 540
AlwNI CAG_nnn'CTG
Cuts at: 0 564 672
Size: 564 108
BamHI G'GATC_C
Cuts at: 0 404 672
Size: 404 268
BanI G'GyrC_C
Cuts at: 0 98 157 672
Size: 98 59 515
Fragments arranged by size:
515 98 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 516 672
Size: 516 156
BglII A'GATC_T
Cuts at: 0 375 672
Size: 375 297
BmgBI CAC'GTC
Cuts at: 0 621 672
Size: 621 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 672
Size: 670 2
BsaWI w'CCGG_w
Cuts at: 0 231 635 672
Size: 231 404 37
Fragments arranged by size:
404 231 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 532 562 672
Size: 532 30 110
Fragments arranged by size:
532 110 30
BseYI C'CCAG_C
Cuts at: 0 609 672
Size: 609 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 672
Size: 478 194
BsmI GAATG_Cn'
Cuts at: 0 190 672
Size: 190 482
BspEI T'CCGG_A
Cuts at: 0 635 672
Size: 635 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 145 253 572 672
Size: 145 108 319 100
Fragments arranged by size:
319 145 108 100
BsrDI GCAATG_nn'
Cuts at: 0 626 672
Size: 626 46
BstXI CCAn_nnnn'nTGG
Cuts at: 0 168 639 672
Size: 168 471 33
Fragments arranged by size:
471 168 33
BstYI r'GATC_y
Cuts at: 0 375 404 672
Size: 375 29 268
Fragments arranged by size:
375 268 29
BstZ17I GTA'TAC
Cuts at: 0 178 672
Size: 178 494
BtgI C'CryG_G
Cuts at: 0 551 672
Size: 551 121
ClaI AT'CG_AT
Cuts at: 0 431 672
Size: 431 241
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 586 672
Size: 586 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 670 672
Size: 400 270 2
Fragments arranged by size:
400 270 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 412 672
Size: 412 260
EcoO109I rG'GnC_Cy
Cuts at: 0 90 133 672
Size: 90 43 539
Fragments arranged by size:
539 90 43
HaeII r_GCGC'y
Cuts at: 0 423 480 672
Size: 423 57 192
Fragments arranged by size:
423 192 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 532 564 672
Size: 532 32 108
Fragments arranged by size:
532 108 32
KpnI G_GTAC'C
Cuts at: 0 102 672
Size: 102 570
MslI CAynn'nnrTG
Cuts at: 0 114 637 672
Size: 114 523 35
Fragments arranged by size:
523 114 35
MspA1I CmG'CkG
Cuts at: 0 609 672
Size: 609 63
NspI r_CATG'y
Cuts at: 0 113 672
Size: 113 559
PasI CC'CwG_GG
Cuts at: 0 414 672
Size: 414 258
PfoI T'CCnGG_A
Cuts at: 0 347 672
Size: 347 325
PpuMI rG'GwC_Cy
Cuts at: 0 90 133 672
Size: 90 43 539
Fragments arranged by size:
539 90 43
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 475 507 672
Size: 475 32 165
Fragments arranged by size:
475 165 32
PstI C_TGCA'G
Cuts at: 0 156 672
Size: 156 516
RsrII CG'GwC_CG
Cuts at: 0 540 672
Size: 540 132
SfcI C'TryA_G
Cuts at: 0 152 672
Size: 152 520
SphI G_CATG'C
Cuts at: 0 113 672
Size: 113 559
StyI C'CwwG_G
Cuts at: 0 126 167 672
Size: 126 41 505
Fragments arranged by size:
505 126 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 314 427 484 672
Size: 314 113 57 188
Fragments arranged by size:
314 188 113 57
TatI w'GTAC_w
Cuts at: 0 449 461 672
Size: 449 12 211
Fragments arranged by size:
449 211 12
Enzymes that do cut:
AarI AccI Acc65I AcuI AlfI AlwNI BamHI BanI
BbsI BglII BmgBI BpmI BsaWI BsaXI BseYI BsgI
BsmI BspEI BspMI BsrDI BstXI BstYI BstZ17I BtgI
ClaI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I KpnI
MslI MspA1I NspI PasI PfoI PpuMI PsrI PstI
RsrII SfcI SphI StyI TaqII TatI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BanII BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BlpI Bme1580I BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BseRI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII Bsu36I BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI MfeI MluI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI