(Linear) (Six-base) MAPSORT of: KIBB7238.seq  Check: 8199  from: 1  to: 354



KIBB7238.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AleI CACnn'nnGTG

Cuts at:      0      81     354 
   Size:         81     273
 
AlwNI CAG_nnn'CTG

Cuts at:      0     314     354 
   Size:        314      40
 
ApaLI G'TGCA_C

Cuts at:      0      45     354 
   Size:         45     309
 
BanII G_rGCy'C

Cuts at:      0     277     307     354 
   Size:        277      30      47

  Fragments arranged by size: 

                 277      47      30
 
BbvCI CC'TCA_GC

Cuts at:      0     297     354 
   Size:        297      57
 
Bme1580I G_kGCm'C

Cuts at:      0      49      61      73      88     112     354 
   Size:         49      12      12      15      24     242

  Fragments arranged by size: 

                 242      49      24      15      12      12
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     291     354 
   Size:        144     147      63

  Fragments arranged by size: 

                 147     144      63
 
Bpu10I CC'TnA_GC

Cuts at:      0     229     297     354 
   Size:        229      68      57

  Fragments arranged by size: 

                 229      68      57
 
BsaWI w'CCGG_w

Cuts at:      0     142     354 
   Size:        142     212
 
BsiHKAI G_wGCw'C

Cuts at:      0      49     277     354 
   Size:         49     228      77

  Fragments arranged by size: 

                 228      77      49
 
Bsp1286I G_dGCh'C

Cuts at:      0      49      61      73      88     112     277     307     354 
   Size:         49      12      12      15      24     165      30      47

  Fragments arranged by size: 

                 165      49      47      30      24      15      12      12
            
 
BspEI T'CCGG_A

Cuts at:      0     142     354 
   Size:        142     212
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     340     354 
   Size:        340      14
 
BtsI GCAGTG_nn'

Cuts at:      0      31     333     354 
   Size:         31     302      21

  Fragments arranged by size: 

                 302      31      21
 
EaeI y'GGCC_r

Cuts at:      0     330     354 
   Size:        330      24
 
EarI CTCTTCn'nnn_

Cuts at:      0     126     354 
   Size:        126     228
 
EcoICRI GAG'CTC

Cuts at:      0     275     354 
   Size:        275      79
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     291     354 
   Size:        144     147      63

  Fragments arranged by size: 

                 147     144      63
 
EcoO109I rG'GnC_Cy

Cuts at:      0     251     354 
   Size:        251     103
 
MscI TGG'CCA

Cuts at:      0     332     354 
   Size:        332      22
 
MslI CAynn'nnrTG

Cuts at:      0      81     354 
   Size:         81     273
 
MspA1I CmG'CkG

Cuts at:      0     301     311     354 
   Size:        301      10      43

  Fragments arranged by size: 

                 301      43      10
 
PpuMI rG'GwC_Cy

Cuts at:      0     251     354 
   Size:        251     103
 
PstI C_TGCA'G

Cuts at:      0     133     181     205     289     354 
   Size:        133      48      24      84      65

  Fragments arranged by size: 

                 133      84      65      48      24
 
PvuII CAG'CTG

Cuts at:      0     311     354 
   Size:        311      43
 
SacI G_AGCT'C

Cuts at:      0     277     354 
   Size:        277      77
 
SfcI C'TryA_G

Cuts at:      0     129     177     201     285     354 
   Size:        129      48      24      84      69

  Fragments arranged by size: 

                 129      84      69      48      24
 

 Enzymes that do cut:

     AleI    AlwNI    ApaLI    BanII    BbvCI Bme1580I     BpmI   Bpu10I
    BsaWI  BsiHKAI Bsp1286I    BspEI    BstXI     BtsI     EaeI     EarI
  EcoICRI  Eco57MI EcoO109I     MscI     MslI   MspA1I    PpuMI     PstI
    PvuII     SacI     SfcI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI     ApaI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI