(Linear) (Six-base) MAPSORT of: KIBB7237.seq  Check: 3155  from: 1  to: 1443



KIBB7237.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AccI GT'mk_AC

Cuts at:      0     205     395    1443 
   Size:        205     190    1048

  Fragments arranged by size: 

                1048     205     190
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     325    1443 
   Size:        144     181    1118

  Fragments arranged by size: 

                1118     181     144
 
AflIII A'CryG_T

Cuts at:      0    1423    1443 
   Size:       1423      20
 
ApaLI G'TGCA_C

Cuts at:      0     475    1443 
   Size:        475     968
 
ApoI r'AATT_y

Cuts at:      0     130     797    1443 
   Size:        130     667     646

  Fragments arranged by size: 

                 667     646     130
 
BanII G_rGCy'C

Cuts at:      0     549     725    1285    1443 
   Size:        549     176     560     158

  Fragments arranged by size: 

                 560     549     176     158
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1020    1443 
   Size:       1020     423
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     301    1035    1443 
   Size:        301     734     408

  Fragments arranged by size: 

                 734     408     301
 
BlpI GC'TnA_GC

Cuts at:      0     230    1443 
   Size:        230    1213
 
Bme1580I G_kGCm'C

Cuts at:      0     479    1443 
   Size:        479     964
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     420     615    1443 
   Size:        420     195     828

  Fragments arranged by size: 

                 828     420     195
 
Bpu10I CC'TnA_GC

Cuts at:      0     540    1443 
   Size:        540     903
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     181    1443 
   Size:        181    1262
 
BsaBI GATnn'nnATC

Cuts at:      0     743    1443 
   Size:        743     700
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     393     423    1443 
   Size:        393      30    1020

  Fragments arranged by size: 

                1020     393      30
 
BseYI C'CCAG_C

Cuts at:      0     616     960    1443 
   Size:        616     344     483

  Fragments arranged by size: 

                 616     483     344
 
BsiHKAI G_wGCw'C

Cuts at:      0      48     479    1285    1443 
   Size:         48     431     806     158

  Fragments arranged by size: 

                 806     431     158      48
 
BsmI GAATG_Cn'

Cuts at:      0     713    1443 
   Size:        713     730
 
Bsp1286I G_dGCh'C

Cuts at:      0      48     479     549     725    1285    1443 
   Size:         48     431      70     176     560     158

  Fragments arranged by size: 

                 560     431     176     158      70      48
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1361    1443 
   Size:       1361      82
 
BsrGI T'GTAC_A

Cuts at:      0    1426    1443 
   Size:       1426      17
 
BstYI r'GATC_y

Cuts at:      0    1316    1443 
   Size:       1316     127
 
BstZ17I GTA'TAC

Cuts at:      0     206    1443 
   Size:        206    1237
 
BtsI GCAGTG_nn'

Cuts at:      0    1300    1443 
   Size:       1300     143
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     659     694    1443 
   Size:        659      35     749

  Fragments arranged by size: 

                 749     659      35
 
DraI TTT'AAA

Cuts at:      0     984     995    1134    1443 
   Size:        984      11     139     309

  Fragments arranged by size: 

                 984     309     139      11
 
EarI CTCTTCn'nnn_

Cuts at:      0    1157    1443 
   Size:       1157     286
 
EcoICRI GAG'CTC

Cuts at:      0    1283    1443 
   Size:       1283     160
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     325     420     615    1443 
   Size:        144     181      95     195     828

  Fragments arranged by size: 

                 828     195     181     144      95
 
EcoRI G'AATT_C

Cuts at:      0     130     797    1443 
   Size:        130     667     646

  Fragments arranged by size: 

                 667     646     130
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     174     930    1426    1443 
   Size:        174     756     496      17

  Fragments arranged by size: 

                 756     496     174      17
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1209    1241    1272    1304    1443 
   Size:       1209      32      31      32     139

  Fragments arranged by size: 

                1209     139      32      32      31
 
HincII GTy'rAC

Cuts at:      0     586    1443 
   Size:        586     857
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     546    1443 
   Size:        546     897
 
MslI CAynn'nnrTG

Cuts at:      0     196     532     554    1443 
   Size:        196     336      22     889

  Fragments arranged by size: 

                 889     336     196      22
 
NspI r_CATG'y

Cuts at:      0    1307    1427    1443 
   Size:       1307     120      16

  Fragments arranged by size: 

                1307     120      16
 
PciI A'CATG_T

Cuts at:      0    1423    1443 
   Size:       1423      20
 
PsiI TTA'TAA

Cuts at:      0     428    1199    1235    1443 
   Size:        428     771      36     208

  Fragments arranged by size: 

                 771     428     208      36
 
PstI C_TGCA'G

Cuts at:      0    1295    1443 
   Size:       1295     148
 
SacI G_AGCT'C

Cuts at:      0    1285    1443 
   Size:       1285     158
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1157    1443 
   Size:       1157     286
 
SfcI C'TryA_G

Cuts at:      0    1291    1443 
   Size:       1291     152
 
SspI AAT'ATT

Cuts at:      0    1251    1380    1443 
   Size:       1251     129      63

  Fragments arranged by size: 

                1251     129      63
 
StyI C'CwwG_G

Cuts at:      0     622     725    1168    1443 
   Size:        622     103     443     275

  Fragments arranged by size: 

                 622     443     275     103
 
SwaI ATTT'AAAT

Cuts at:      0    1134    1443 
   Size:       1134     309
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      81    1443 
   Size:         81    1362
 
TatI w'GTAC_w

Cuts at:      0    1383    1426    1443 
   Size:       1383      43      17

  Fragments arranged by size: 

                1383      43      17
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     393     425    1443 
   Size:        393      32    1018

  Fragments arranged by size: 

                1018     393      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1035    1443 
   Size:       1035     408
 
XmnI GAAnn'nnTTC

Cuts at:      0    1406    1443 
   Size:       1406      37
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    ApaLI     ApoI    BanII     BbsI    BciVI
     BlpI Bme1580I     BpmI   Bpu10I     BsaI    BsaBI    BsaXI    BseYI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrGI    BstYI  BstZ17I     BtsI
    CspCI     DraI     EarI  EcoICRI  Eco57MI    EcoRI     FalI    Hin4I
   HincII     MmeI     MslI     NspI     PciI     PsiI     PstI     SacI
     SapI     SfcI     SspI     StyI     SwaI    TaqII     TatI     TstI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
     BclI    BfrBI     BglI    BglII    BmgBI     BmrI     BmtI     BplI
    BpuEI    BsaAI    BsaHI    BsaWI    BseRI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI   Bsu36I     BtgI    BtgZI     ClaI
   DraIII     DrdI     EaeI     EagI     EciI    EcoNI EcoO109I    EcoRV
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI  Tth111I     XbaI     XhoI
     XmaI     ZraI