Restriction map for
pF1KB7232
(Linear) (Six-base) MAPSORT of: KIBB7232.seq Check: 1782 from: 1 to: 1188
KIBB7232.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 596 1188
Size: 596 592
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 897 1188
Size: 433 464 291
Fragments arranged by size:
464 433 291
AflII C'TTAA_G
Cuts at: 0 874 1188
Size: 874 314
AflIII A'CryG_T
Cuts at: 0 726 1188
Size: 726 462
AgeI A'CCGG_T
Cuts at: 0 290 1188
Size: 290 898
AhdI GACnn_n'nnGTC
Cuts at: 0 17 1188
Size: 17 1171
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 763 1188
Size: 763 425
ApoI r'AATT_y
Cuts at: 0 553 1188
Size: 553 635
AseI AT'TA_AT
Cuts at: 0 512 1188
Size: 512 676
AvaI C'yCGr_G
Cuts at: 0 951 1188
Size: 951 237
BanI G'GyrC_C
Cuts at: 0 222 1188
Size: 222 966
BanII G_rGCy'C
Cuts at: 0 274 1188
Size: 274 914
BbsI GAAGACnn'nnnn_
Cuts at: 0 13 1188
Size: 13 1175
BbvCI CC'TCA_GC
Cuts at: 0 438 1188
Size: 438 750
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 78 112 1188
Size: 78 34 1076
Fragments arranged by size:
1076 78 34
BclI T'GATC_A
Cuts at: 0 1002 1188
Size: 1002 186
BlpI GC'TnA_GC
Cuts at: 0 172 1188
Size: 172 1016
Bme1580I G_kGCm'C
Cuts at: 0 227 1188
Size: 227 961
BmgBI CAC'GTC
Cuts at: 0 921 1188
Size: 921 267
Bpu10I CC'TnA_GC
Cuts at: 0 148 438 1188
Size: 148 290 750
Fragments arranged by size:
750 290 148
BsaI GGTCTCn'nnnn_
Cuts at: 0 189 1188
Size: 189 999
BsaAI yAC'GTr
Cuts at: 0 906 1188
Size: 906 282
BsaHI Gr'CG_yC
Cuts at: 0 106 215 1188
Size: 106 109 973
Fragments arranged by size:
973 109 106
BsaWI w'CCGG_w
Cuts at: 0 75 290 1188
Size: 75 215 898
Fragments arranged by size:
898 215 75
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 439 715 1188
Size: 439 276 473
Fragments arranged by size:
473 439 276
BseYI C'CCAG_C
Cuts at: 0 81 128 205 1188
Size: 81 47 77 983
Fragments arranged by size:
983 81 77 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 635 1188
Size: 635 553
BsmI GAATG_Cn'
Cuts at: 0 603 988 1188
Size: 603 385 200
Fragments arranged by size:
603 385 200
Bsp1286I G_dGCh'C
Cuts at: 0 227 274 1188
Size: 227 47 914
Fragments arranged by size:
914 227 47
BspEI T'CCGG_A
Cuts at: 0 75 1188
Size: 75 1113
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 543 596 731 1188
Size: 543 53 135 457
Fragments arranged by size:
543 457 135 53
BsrDI GCAATG_nn'
Cuts at: 0 784 1188
Size: 784 404
BsrFI r'CCGG_y
Cuts at: 0 290 1188
Size: 290 898
BstXI CCAn_nnnn'nTGG
Cuts at: 0 573 1188
Size: 573 615
Bsu36I CC'TnA_GG
Cuts at: 0 1070 1188
Size: 1070 118
BtgI C'CryG_G
Cuts at: 0 218 419 669 975 1188
Size: 218 201 250 306 213
Fragments arranged by size:
306 250 218 213 201
EaeI y'GGCC_r
Cuts at: 0 5 801 1188
Size: 5 796 387
Fragments arranged by size:
796 387 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 897 1188
Size: 433 464 291
Fragments arranged by size:
464 433 291
EcoO109I rG'GnC_Cy
Cuts at: 0 11 45 144 1188
Size: 11 34 99 1044
Fragments arranged by size:
1044 99 34 11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 761 1188
Size: 761 427
FspI TGC'GCA
Cuts at: 0 328 1188
Size: 328 860
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3 35 930 962 1188
Size: 3 32 895 32 226
Fragments arranged by size:
895 226 32 32 3
HincII GTy'rAC
Cuts at: 0 1047 1056 1188
Size: 1047 9 132
Fragments arranged by size:
1047 132 9
HpaI GTT'AAC
Cuts at: 0 1056 1188
Size: 1056 132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 1188
Size: 275 913
MscI TGG'CCA
Cuts at: 0 803 1188
Size: 803 385
MslI CAynn'nnrTG
Cuts at: 0 755 1165 1188
Size: 755 410 23
Fragments arranged by size:
755 410 23
MspA1I CmG'CkG
Cuts at: 0 17 634 767 864 884 1188
Size: 17 617 133 97 20 304
Fragments arranged by size:
617 304 133 97 20 17
NcoI C'CATG_G
Cuts at: 0 669 1188
Size: 669 519
NspI r_CATG'y
Cuts at: 0 180 237 730 1079 1188
Size: 180 57 493 349 109
Fragments arranged by size:
493 349 180 109 57
PciI A'CATG_T
Cuts at: 0 726 1188
Size: 726 462
PflMI CCAn_nnn'nTGG
Cuts at: 0 294 1188
Size: 294 894
PpuMI rG'GwC_Cy
Cuts at: 0 11 45 144 1188
Size: 11 34 99 1044
Fragments arranged by size:
1044 99 34 11
PshAI GACnn'nnGTC
Cuts at: 0 1042 1188
Size: 1042 146
PvuII CAG'CTG
Cuts at: 0 767 884 1188
Size: 767 117 304
Fragments arranged by size:
767 304 117
SanDI GG'GwC_CC
Cuts at: 0 11 1188
Size: 11 1177
SexAI A'CCwGG_T
Cuts at: 0 580 1188
Size: 580 608
SmaI CCC'GGG
Cuts at: 0 953 1188
Size: 953 235
SmlI C'TyrA_G
Cuts at: 0 874 1188
Size: 874 314
SphI G_CATG'C
Cuts at: 0 1079 1188
Size: 1079 109
SspI AAT'ATT
Cuts at: 0 516 1188
Size: 516 672
StyI C'CwwG_G
Cuts at: 0 669 1188
Size: 669 519
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1169 1188
Size: 1169 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 665 697 1188
Size: 665 32 491
Fragments arranged by size:
665 491 32
Tth111I GACn'n_nGTC
Cuts at: 0 142 1188
Size: 142 1046
XmaI C'CCGG_G
Cuts at: 0 951 1188
Size: 951 237
Enzymes that do cut:
AarI AcuI AflII AflIII AgeI AhdI AlfI ApoI
AseI AvaI BanI BanII BbsI BbvCI BcgI BclI
BlpI Bme1580I BmgBI Bpu10I BsaI BsaAI BsaHI BsaWI
BseRI BseYI BsgI BsmI Bsp1286I BspEI BspMI BsrDI
BsrFI BstXI Bsu36I BtgI EaeI Eco57MI EcoO109I FalI
FspI Hin4I HincII HpaI MmeI MscI MslI MspA1I
NcoI NspI PciI PflMI PpuMI PshAI PvuII SanDI
SexAI SmaI SmlI SphI SspI StyI TaqII TstI
Tth111I XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AleI AloI AlwNI
ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BbeI
BciVI BfrBI BglI BglII BmrI BmtI BplI BpmI
BpuEI BsaBI BsaXI BsiEI BsiHKAI BsiWI BsmBI BspHI
BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI
FspAI HaeII HindIII KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI StuI SwaI TatI XbaI XcmI XhoI
XmnI ZraI