(Linear) (Six-base) MAPSORT of: KIBB7230.seq  Check: 5893  from: 1  to: 1053



KIBB7230.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     997    1053 
   Size:        997      56
 
AflIII A'CryG_T

Cuts at:      0     925    1053 
   Size:        925     128
 
AlwNI CAG_nnn'CTG

Cuts at:      0     599    1053 
   Size:        599     454
 
ApoI r'AATT_y

Cuts at:      0      64     692     998    1053 
   Size:         64     628     306      55

  Fragments arranged by size: 

                 628     306      64      55
 
BamHI G'GATC_C

Cuts at:      0     154     852    1053 
   Size:        154     698     201

  Fragments arranged by size: 

                 698     201     154
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     528    1053 
   Size:        528     525
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     714    1053 
   Size:        714     339
 
BclI T'GATC_A

Cuts at:      0     302    1053 
   Size:        302     751
 
BglII A'GATC_T

Cuts at:      0      91     112    1053 
   Size:         91      21     941

  Fragments arranged by size: 

                 941      91      21
 
BlpI GC'TnA_GC

Cuts at:      0     376    1053 
   Size:        376     677
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     598    1053 
   Size:        598     455
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     533    1053 
   Size:        533     520
 
BsiHKAI G_wGCw'C

Cuts at:      0     975    1053 
   Size:        975      78
 
Bsp1286I G_dGCh'C

Cuts at:      0     975    1053 
   Size:        975      78
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     637    1053 
   Size:        637     416
 
BstYI r'GATC_y

Cuts at:      0      91     112     154     852    1053 
   Size:         91      21      42     698     201

  Fragments arranged by size: 

                 698     201      91      42      21
 
BtgI C'CryG_G

Cuts at:      0     251    1053 
   Size:        251     802
 
EaeI y'GGCC_r

Cuts at:      0     254     410    1053 
   Size:        254     156     643

  Fragments arranged by size: 

                 643     254     156
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     533     997    1053 
   Size:        533     464      56

  Fragments arranged by size: 

                 533     464      56
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     269     301    1053 
   Size:        269      32     752

  Fragments arranged by size: 

                 752     269      32
 
MfeI C'AATT_G

Cuts at:      0      69    1053 
   Size:         69     984
 
MscI TGG'CCA

Cuts at:      0     256     412    1053 
   Size:        256     156     641

  Fragments arranged by size: 

                 641     256     156
 
MslI CAynn'nnrTG

Cuts at:      0     286     719    1053 
   Size:        286     433     334

  Fragments arranged by size: 

                 433     334     286
 
NcoI C'CATG_G

Cuts at:      0     251    1053 
   Size:        251     802
 
NspI r_CATG'y

Cuts at:      0     718     929    1053 
   Size:        718     211     124

  Fragments arranged by size: 

                 718     211     124
 
PciI A'CATG_T

Cuts at:      0     925    1053 
   Size:        925     128
 
SfcI C'TryA_G

Cuts at:      0     878    1053 
   Size:        878     175
 
SspI AAT'ATT

Cuts at:      0     173    1053 
   Size:        173     880
 
StuI AGG'CCT

Cuts at:      0     547    1053 
   Size:        547     506
 
StyI C'CwwG_G

Cuts at:      0     251    1053 
   Size:        251     802
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     109     495    1053 
   Size:        109     386     558

  Fragments arranged by size: 

                 558     386     109
 
TatI w'GTAC_w

Cuts at:      0     980    1053 
   Size:        980      73
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     915     947    1053 
   Size:        915      32     106

  Fragments arranged by size: 

                 915     106      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     639    1053 
   Size:        639     414
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI    BamHI     BbsI    BciVI     BclI
    BglII     BlpI     BmrI     BpmI  BsiHKAI Bsp1286I    BspMI    BstYI
     BtgI     EaeI  Eco57MI    Hin4I     MfeI     MscI     MslI     NcoI
     NspI     PciI     SfcI     SspI     StuI     StyI    TaqII     TatI
     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI
     BcgI    BfrBI     BglI Bme1580I    BmgBI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SwaI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI