Restriction map for
pF1KB7229
(Linear) (Six-base) MAPSORT of: KIBB7229.seq Check: 2038 from: 1 to: 1119
KIBB7229.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 697 1119
Size: 697 422
AccI GT'mk_AC
Cuts at: 0 569 1119
Size: 569 550
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 309 718 735 1119
Size: 309 409 17 384
Fragments arranged by size:
409 384 309 17
AleI CACnn'nnGTG
Cuts at: 0 140 544 1003 1119
Size: 140 404 459 116
Fragments arranged by size:
459 404 140 116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 169 208 484 1119
Size: 169 39 276 635
Fragments arranged by size:
635 276 169 39
ApaI G_GGCC'C
Cuts at: 0 70 1119
Size: 70 1049
AvaI C'yCGr_G
Cuts at: 0 613 1119
Size: 613 506
BanI G'GyrC_C
Cuts at: 0 28 1119
Size: 28 1091
BanII G_rGCy'C
Cuts at: 0 70 1119
Size: 70 1049
BbsI GAAGACnn'nnnn_
Cuts at: 0 639 1119
Size: 639 480
BlpI GC'TnA_GC
Cuts at: 0 713 1119
Size: 713 406
Bme1580I G_kGCm'C
Cuts at: 0 70 1119
Size: 70 1049
BmrI ACTGGGnnnn_n'
Cuts at: 0 772 1119
Size: 772 347
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 872 1119
Size: 872 247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 613 651 1119
Size: 424 189 38 468
Fragments arranged by size:
468 424 189 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 96 580 1119
Size: 96 484 539
Fragments arranged by size:
539 484 96
BsaAI yAC'GTr
Cuts at: 0 468 1119
Size: 468 651
BsaBI GATnn'nnATC
Cuts at: 0 256 682 1119
Size: 256 426 437
Fragments arranged by size:
437 426 256
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 361 394 1119
Size: 361 33 725
Fragments arranged by size:
725 361 33
BseYI C'CCAG_C
Cuts at: 0 1014 1119
Size: 1014 105
BsmI GAATG_Cn'
Cuts at: 0 528 595 1119
Size: 528 67 524
Fragments arranged by size:
528 524 67
Bsp1286I G_dGCh'C
Cuts at: 0 70 1119
Size: 70 1049
BspHI T'CATG_A
Cuts at: 0 863 1119
Size: 863 256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 147 697 1119
Size: 147 550 422
Fragments arranged by size:
550 422 147
BsrDI GCAATG_nn'
Cuts at: 0 291 1119
Size: 291 828
BsrGI T'GTAC_A
Cuts at: 0 179 1119
Size: 179 940
BssSI C'ACGA_G
Cuts at: 0 136 1119
Size: 136 983
BstEII G'GTnAC_C
Cuts at: 0 702 961 1119
Size: 702 259 158
Fragments arranged by size:
702 259 158
BstXI CCAn_nnnn'nTGG
Cuts at: 0 142 1119
Size: 142 977
BtgI C'CryG_G
Cuts at: 0 95 212 332 555 929 1119
Size: 95 117 120 223 374 190
Fragments arranged by size:
374 223 190 120 117 95
BtsI GCAGTG_nn'
Cuts at: 0 472 492 985 1088 1119
Size: 472 20 493 103 31
Fragments arranged by size:
493 472 103 31 20
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 461 496 1119
Size: 461 35 623
Fragments arranged by size:
623 461 35
DraIII CAC_nnn'GTG
Cuts at: 0 498 1104 1119
Size: 498 606 15
Fragments arranged by size:
606 498 15
EaeI y'GGCC_r
Cuts at: 0 500 1119
Size: 500 619
EarI CTCTTCn'nnn_
Cuts at: 0 394 594 1119
Size: 394 200 525
Fragments arranged by size:
525 394 200
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 309 424 613 651 718 735 1119
Size: 309 115 189 38 67 17 384
Fragments arranged by size:
384 309 189 115 67 38 17
EcoO109I rG'GnC_Cy
Cuts at: 0 404 720 1119
Size: 404 316 399
Fragments arranged by size:
404 399 316
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 348 380 388 420 1119
Size: 348 32 8 32 699
Fragments arranged by size:
699 348 32 32 8
HincII GTy'rAC
Cuts at: 0 570 1119
Size: 570 549
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 788 1119
Size: 108 680 331
Fragments arranged by size:
680 331 108
MscI TGG'CCA
Cuts at: 0 502 1119
Size: 502 617
MslI CAynn'nnrTG
Cuts at: 0 140 544 1003 1119
Size: 140 404 459 116
Fragments arranged by size:
459 404 140 116
MspA1I CmG'CkG
Cuts at: 0 770 1119
Size: 770 349
NcoI C'CATG_G
Cuts at: 0 95 555 1119
Size: 95 460 564
Fragments arranged by size:
564 460 95
NspI r_CATG'y
Cuts at: 0 156 907 1119
Size: 156 751 212
Fragments arranged by size:
751 212 156
PflMI CCAn_nnn'nTGG
Cuts at: 0 498 770 1119
Size: 498 272 349
Fragments arranged by size:
498 349 272
PfoI T'CCnGG_A
Cuts at: 0 844 947 1119
Size: 844 103 172
Fragments arranged by size:
844 172 103
PmlI CAC'GTG
Cuts at: 0 468 1119
Size: 468 651
PpuMI rG'GwC_Cy
Cuts at: 0 404 720 1119
Size: 404 316 399
Fragments arranged by size:
404 399 316
PshAI GACnn'nnGTC
Cuts at: 0 800 1119
Size: 800 319
PspOMI G'GGCC_C
Cuts at: 0 66 1119
Size: 66 1053
PvuII CAG'CTG
Cuts at: 0 770 1119
Size: 770 349
SalI G'TCGA_C
Cuts at: 0 568 1119
Size: 568 551
SexAI A'CCwGG_T
Cuts at: 0 313 1119
Size: 313 806
SmaI CCC'GGG
Cuts at: 0 615 1119
Size: 615 504
SphI G_CATG'C
Cuts at: 0 156 1119
Size: 156 963
StuI AGG'CCT
Cuts at: 0 76 742 1025 1119
Size: 76 666 283 94
Fragments arranged by size:
666 283 94 76
StyI C'CwwG_G
Cuts at: 0 62 95 555 1119
Size: 62 33 460 564
Fragments arranged by size:
564 460 62 33
TatI w'GTAC_w
Cuts at: 0 179 1119
Size: 179 940
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 929 961 1119
Size: 929 32 158
Fragments arranged by size:
929 158 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 417 1119
Size: 417 702
XmaI C'CCGG_G
Cuts at: 0 613 1119
Size: 613 506
Enzymes that do cut:
AarI AccI AcuI AleI AlfI ApaI AvaI BanI
BanII BbsI BlpI Bme1580I BmrI BplI BpmI BsaI
BsaAI BsaBI BseRI BseYI BsmI Bsp1286I BspHI BspMI
BsrDI BsrGI BssSI BstEII BstXI BtgI BtsI CspCI
DraIII EaeI EarI Eco57MI EcoO109I Hin4I HincII MmeI
MscI MslI MspA1I NcoI NspI PflMI PfoI PmlI
PpuMI PshAI PspOMI PvuII SalI SexAI SmaI SphI
StuI StyI TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmgBI BmtI Bpu10I BpuEI BsaHI BsaWI
BsaXI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI
BsrFI BssHII BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI
ClaI DraI DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PpiI PsiI PspXI PsrI PstI PvuI RsrII SacI
SacII SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SrfI SspI SwaI
TaqII Tth111I XbaI XhoI XmnI ZraI