(Linear) (Six-base) MAPSORT of: KIBB7227.seq  Check: 4801  from: 1  to: 660



KIBB7227.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AfeI AGC'GCT

Cuts at:      0     355     660 
   Size:        355     305
 
ApoI r'AATT_y

Cuts at:      0     124     660 
   Size:        124     536
 
AvaI C'yCGr_G

Cuts at:      0     323     660 
   Size:        323     337
 
BanII G_rGCy'C

Cuts at:      0     313     660 
   Size:        313     347
 
BmgBI CAC'GTC

Cuts at:      0     634     660 
   Size:        634      26
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     660 
   Size:        224     436
 
BseYI C'CCAG_C

Cuts at:      0     483     660 
   Size:        483     177
 
Bsp1286I G_dGCh'C

Cuts at:      0     313     660 
   Size:        313     347
 
BsrDI GCAATG_nn'

Cuts at:      0     229     660 
   Size:        229     431
 
BsrGI T'GTAC_A

Cuts at:      0     344     660 
   Size:        344     316
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     451     660 
   Size:        451     209
 
BtgI C'CryG_G

Cuts at:      0     129     305     432     660 
   Size:        129     176     127     228

  Fragments arranged by size: 

                 228     176     129     127
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     660 
   Size:        224     436
 
HaeII r_GCGC'y

Cuts at:      0     357     660 
   Size:        357     303
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     317     349     660 
   Size:        317      32     311

  Fragments arranged by size: 

                 317     311      32
 
MfeI C'AATT_G

Cuts at:      0      14     660 
   Size:         14     646
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     593     660 
   Size:        593      67
 
MslI CAynn'nnrTG

Cuts at:      0     276     449     660 
   Size:        276     173     211

  Fragments arranged by size: 

                 276     211     173
 
MspA1I CmG'CkG

Cuts at:      0     434     660 
   Size:        434     226
 
NcoI C'CATG_G

Cuts at:      0     129     660 
   Size:        129     531
 
NdeI CA'TA_TG

Cuts at:      0     277     660 
   Size:        277     383
 
PsiI TTA'TAA

Cuts at:      0      50     440     660 
   Size:         50     390     220

  Fragments arranged by size: 

                 390     220      50
 
SacII CC_GC'GG

Cuts at:      0     435     660 
   Size:        435     225
 
SmlI C'TyrA_G

Cuts at:      0     323     660 
   Size:        323     337
 
SpeI A'CTAG_T

Cuts at:      0     498     660 
   Size:        498     162
 
StyI C'CwwG_G

Cuts at:      0     129     660 
   Size:        129     531
 
TatI w'GTAC_w

Cuts at:      0     344     660 
   Size:        344     316
 
XbaI T'CTAG_A

Cuts at:      0     118     660 
   Size:        118     542
 
XhoI C'TCGA_G

Cuts at:      0     323     660 
   Size:        323     337
 

 Enzymes that do cut:

     AfeI     ApoI     AvaI    BanII    BmgBI     BpmI    BseYI Bsp1286I
    BsrDI    BsrGI    BstXI     BtgI  Eco57MI    HaeII    Hin4I     MfeI
     MmeI     MslI   MspA1I     NcoI     NdeI     PsiI    SacII     SmlI
     SpeI     StyI     TatI     XbaI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MluI     MscI     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI  Tth111I     XcmI     XmaI     XmnI     ZraI