(Linear) (Six-base) MAPSORT of: KIBB7225.seq  Check: 9341  from: 1  to: 786



KIBB7225.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     191     566     786 
   Size:         34     157     375     220

  Fragments arranged by size: 

                 375     220     157      34
 
ApaI G_GGCC'C

Cuts at:      0     474     786 
   Size:        474     312
 
ApoI r'AATT_y

Cuts at:      0     742     786 
   Size:        742      44
 
AseI AT'TA_AT

Cuts at:      0     113     786 
   Size:        113     673
 
AvaI C'yCGr_G

Cuts at:      0     443     786 
   Size:        443     343
 
BanII G_rGCy'C

Cuts at:      0     474     786 
   Size:        474     312
 
Bme1580I G_kGCm'C

Cuts at:      0     474     786 
   Size:        474     312
 
BmgBI CAC'GTC

Cuts at:      0     634     786 
   Size:        634     152
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     113     786 
   Size:        113     673
 
BsaWI w'CCGG_w

Cuts at:      0     479     786 
   Size:        479     307
 
BsmI GAATG_Cn'

Cuts at:      0     601     786 
   Size:        601     185
 
Bsp1286I G_dGCh'C

Cuts at:      0     474     786 
   Size:        474     312
 
BsrDI GCAATG_nn'

Cuts at:      0     104     786 
   Size:        104     682
 
BsrGI T'GTAC_A

Cuts at:      0     667     786 
   Size:        667     119
 
BstYI r'GATC_y

Cuts at:      0     685     786 
   Size:        685     101
 
EarI CTCTTCn'nnn_

Cuts at:      0      31     786 
   Size:         31     755
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     191     566     786 
   Size:         34     157     375     220

  Fragments arranged by size: 

                 375     220     157      34
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     443     475     786 
   Size:        443      32     311

  Fragments arranged by size: 

                 443     311      32
 
HincII GTy'rAC

Cuts at:      0     416     786 
   Size:        416     370
 
MspA1I CmG'CkG

Cuts at:      0     611     786 
   Size:        611     175
 
NdeI CA'TA_TG

Cuts at:      0     291     786 
   Size:        291     495
 
PspOMI G'GGCC_C

Cuts at:      0     470     786 
   Size:        470     316
 
PspXI vC'TCGA_Gb

Cuts at:      0     443     786 
   Size:        443     343
 
PvuII CAG'CTG

Cuts at:      0     611     786 
   Size:        611     175
 
ScaI AGT'ACT

Cuts at:      0     268     786 
   Size:        268     518
 
SmlI C'TyrA_G

Cuts at:      0     443     786 
   Size:        443     343
 
StyI C'CwwG_G

Cuts at:      0     698     786 
   Size:        698      88
 
TatI w'GTAC_w

Cuts at:      0     266     667     748     786 
   Size:        266     401      81      38

  Fragments arranged by size: 

                 401     266      81      38
 
XhoI C'TCGA_G

Cuts at:      0     443     786 
   Size:        443     343
 

 Enzymes that do cut:

     AcuI     ApaI     ApoI     AseI     AvaI    BanII Bme1580I    BmgBI
     BmrI    BsaWI     BsmI Bsp1286I    BsrDI    BsrGI    BstYI     EarI
  Eco57MI    Hin4I   HincII   MspA1I     NdeI   PspOMI    PspXI    PvuII
     ScaI     SmlI     StyI     TatI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI