Restriction map for
pF1KB7224
(Linear) (Six-base) MAPSORT of: KIBB7224.seq Check: 7343 from: 1 to: 1068
KIBB7224.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 37 174 1068
Size: 37 137 894
Fragments arranged by size:
894 137 37
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 893 1068
Size: 353 540 175
Fragments arranged by size:
540 353 175
AleI CACnn'nnGTG
Cuts at: 0 451 1068
Size: 451 617
ApaI G_GGCC'C
Cuts at: 0 351 579 1068
Size: 351 228 489
Fragments arranged by size:
489 351 228
ApaLI G'TGCA_C
Cuts at: 0 308 1068
Size: 308 760
AvaI C'yCGr_G
Cuts at: 0 566 1068
Size: 566 502
BanII G_rGCy'C
Cuts at: 0 351 437 579 1068
Size: 351 86 142 489
Fragments arranged by size:
489 351 142 86
BbvCI CC'TCA_GC
Cuts at: 0 193 517 715 865 1068
Size: 193 324 198 150 203
Fragments arranged by size:
324 203 198 193 150
BclI T'GATC_A
Cuts at: 0 737 1068
Size: 737 331
BglI GCCn_nnn'nGGC
Cuts at: 0 511 1068
Size: 511 557
Bme1580I G_kGCm'C
Cuts at: 0 312 351 579 899 1068
Size: 312 39 228 320 169
Fragments arranged by size:
320 312 228 169 39
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 645 1068
Size: 645 423
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 615 1068
Size: 615 453
Bpu10I CC'TnA_GC
Cuts at: 0 193 517 715 865 1068
Size: 193 324 198 150 203
Fragments arranged by size:
324 203 198 193 150
BsaI GGTCTCn'nnnn_
Cuts at: 0 10 1068
Size: 10 1058
BsaHI Gr'CG_yC
Cuts at: 0 137 230 1068
Size: 137 93 838
Fragments arranged by size:
838 137 93
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 84 114 399 429 1068
Size: 84 30 285 30 639
Fragments arranged by size:
639 285 84 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 431 775 947 1068
Size: 431 344 172 121
Fragments arranged by size:
431 344 172 121
BseYI C'CCAG_C
Cuts at: 0 51 530 1068
Size: 51 479 538
Fragments arranged by size:
538 479 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 1068
Size: 373 695
BsiHKAI G_wGCw'C
Cuts at: 0 312 462 1068
Size: 312 150 606
Fragments arranged by size:
606 312 150
Bsp1286I G_dGCh'C
Cuts at: 0 312 351 437 462 579 899 1068
Size: 312 39 86 25 117 320 169
Fragments arranged by size:
320 312 169 117 86 39 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 23 37 174 1068
Size: 23 14 137 894
Fragments arranged by size:
894 137 23 14
BssSI C'ACGA_G
Cuts at: 0 727 1068
Size: 727 341
BstAPI GCAn_nnn'nTGC
Cuts at: 0 272 1035 1068
Size: 272 763 33
Fragments arranged by size:
763 272 33
BstEII G'GTnAC_C
Cuts at: 0 24 1068
Size: 24 1044
BstXI CCAn_nnnn'nTGG
Cuts at: 0 453 1068
Size: 453 615
BstYI r'GATC_y
Cuts at: 0 1005 1068
Size: 1005 63
Bsu36I CC'TnA_GG
Cuts at: 0 754 1068
Size: 754 314
BtgI C'CryG_G
Cuts at: 0 332 671 1068
Size: 332 339 397
Fragments arranged by size:
397 339 332
BtsI GCAGTG_nn'
Cuts at: 0 498 1068
Size: 498 570
DrdI GACnn_nn'nnGTC
Cuts at: 0 255 1068
Size: 255 813
EaeI y'GGCC_r
Cuts at: 0 162 1068
Size: 162 906
EarI CTCTTCn'nnn_
Cuts at: 0 590 1068
Size: 590 478
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 787 940 1068
Size: 787 153 128
Fragments arranged by size:
787 153 128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 615 893 1068
Size: 353 262 278 175
Fragments arranged by size:
353 278 262 175
EcoNI CCTnn'n_nnAGG
Cuts at: 0 368 757 1068
Size: 368 389 311
Fragments arranged by size:
389 368 311
EcoO109I rG'GnC_Cy
Cuts at: 0 35 347 348 467 1068
Size: 35 312 1 119 601
Fragments arranged by size:
601 312 119 35 1
FspI TGC'GCA
Cuts at: 0 276 1068
Size: 276 792
HaeII r_GCGC'y
Cuts at: 0 517 736 1068
Size: 517 219 332
Fragments arranged by size:
517 332 219
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 399 431 809 841 1068
Size: 181 32 186 32 378 32 227
Fragments arranged by size:
378 227 186 181 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1022 1068
Size: 1022 46
MscI TGG'CCA
Cuts at: 0 164 1068
Size: 164 904
MslI CAynn'nnrTG
Cuts at: 0 397 451 1068
Size: 397 54 617
Fragments arranged by size:
617 397 54
MspA1I CmG'CkG
Cuts at: 0 653 690 719 1068
Size: 653 37 29 349
Fragments arranged by size:
653 349 37 29
NcoI C'CATG_G
Cuts at: 0 332 671 1068
Size: 332 339 397
Fragments arranged by size:
397 339 332
PasI CC'CwG_GG
Cuts at: 0 382 1068
Size: 382 686
PflMI CCAn_nnn'nTGG
Cuts at: 0 672 1068
Size: 672 396
PfoI T'CCnGG_A
Cuts at: 0 224 1068
Size: 224 844
PpuMI rG'GwC_Cy
Cuts at: 0 35 467 1068
Size: 35 432 601
Fragments arranged by size:
601 432 35
PspOMI G'GGCC_C
Cuts at: 0 347 575 1068
Size: 347 228 493
Fragments arranged by size:
493 347 228
PstI C_TGCA'G
Cuts at: 0 34 1068
Size: 34 1034
PvuII CAG'CTG
Cuts at: 0 719 1068
Size: 719 349
SbfI CC_TGCA'GG
Cuts at: 0 34 1068
Size: 34 1034
SfcI C'TryA_G
Cuts at: 0 30 472 1068
Size: 30 442 596
Fragments arranged by size:
596 442 30
SmaI CCC'GGG
Cuts at: 0 568 1068
Size: 568 500
StyI C'CwwG_G
Cuts at: 0 233 332 671 978 1009 1068
Size: 233 99 339 307 31 59
Fragments arranged by size:
339 307 233 99 59 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 445 1068
Size: 445 623
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 330 1068
Size: 330 738
XmaI C'CCGG_G
Cuts at: 0 566 1068
Size: 566 502
Enzymes that do cut:
AarI AcuI AleI ApaI ApaLI AvaI BanII BbvCI
BclI BglI Bme1580I BplI BpmI Bpu10I BsaI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DrdI
EaeI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I MmeI MscI MslI MspA1I NcoI PasI PflMI
PfoI PpuMI PspOMI PstI PvuII SbfI SfcI SmaI
StyI TaqII XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BcgI BciVI
BfrBI BglII BlpI BmgBI BmrI BmtI BpuEI BsaAI
BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BstBI BstZ17I BtgZI
ClaI CspCI DraI DraIII EagI EcoICRI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI